Detailed information NTG0678182

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MXH98_RS12170
• Genome accession: NZ_CP095760
• Coordinates: 2506532..2507146 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus velezensis strain ZK-3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2501532..2512146

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MXH98_RS12140 (MXH98_12140)	rpsT	2501835..2502101 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
MXH98_RS12145 (MXH98_12145)	holA	2502118..2503159 (-)	1042	Protein_2349	DNA polymerase III subunit delta	-
MXH98_RS12150 (MXH98_12150)	-	2503199..2503350 (-)	152	Protein_2350	hypothetical protein	-
MXH98_RS12155 (MXH98_12155)	-	2503391..2503525 (+)	135	WP_003152870.1	YqzM family protein	-
MXH98_RS12160 (MXH98_12160)	comEC	2503544..2505895 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MXH98_RS12165 (MXH98_12165)	-	2505896..2506465 (-)	570	WP_003152868.1	ComE operon protein 2	-
MXH98_RS12170 (MXH98_12170)	comEA	2506532..2507146 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
MXH98_RS12175 (MXH98_12175)	comER	2507205..2508026 (+)	822	WP_012118027.1	late competence protein ComER	-
MXH98_RS12180 (MXH98_12180)	-	2508095..2508832 (-)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
MXH98_RS12185 (MXH98_12185)	rsfS	2508829..2509185 (-)	357	WP_007408260.1	ribosome silencing factor	-
MXH98_RS12190 (MXH98_12190)	yqeK	2509202..2509762 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MXH98_RS12195 (MXH98_12195)	-	2509752..2510321 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
MXH98_RS12200 (MXH98_12200)	yhbY	2510332..2510622 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
MXH98_RS12205 (MXH98_12205)	aroE	2510616..2511458 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=678182 MXH98_RS12170 WP_012118026.1 2506532..2507146(-) (comEA) [Bacillus velezensis strain ZK-3]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=678182 MXH98_RS12170 WP_012118026.1 2506532..2507146(-) (comEA) [Bacillus velezensis strain ZK-3]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363