Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L2Y96_RS06585 Genome accession   NZ_CP095741
Coordinates   1472856..1474583 (-) Length   575 a.a.
NCBI ID   WP_247334303.1    Uniprot ID   -
Organism   Luteibacter aegosomaticola strain 335     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1467856..1479583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y96_RS06565 (L2Y96_06565) - 1468618..1469865 (+) 1248 WP_247334287.1 GGDEF domain-containing phosphodiesterase -
  L2Y96_RS06570 (L2Y96_06570) coaE 1469899..1470501 (-) 603 WP_247334289.1 dephospho-CoA kinase -
  L2Y96_RS06575 (L2Y96_06575) pilD 1470498..1471367 (-) 870 WP_247334291.1 A24 family peptidase Machinery gene
  L2Y96_RS06580 (L2Y96_06580) pilC 1471447..1472712 (-) 1266 WP_247334293.1 type II secretion system F family protein Machinery gene
  L2Y96_RS06585 (L2Y96_06585) pilB 1472856..1474583 (-) 1728 WP_247334303.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L2Y96_RS06590 (L2Y96_06590) - 1474733..1475587 (+) 855 WP_247334304.1 glycosyltransferase family 2 protein -
  L2Y96_RS06595 (L2Y96_06595) - 1475584..1476549 (+) 966 WP_247334306.1 glycosyltransferase -
  L2Y96_RS06600 (L2Y96_06600) - 1476709..1477332 (+) 624 WP_247334308.1 class I SAM-dependent methyltransferase -
  L2Y96_RS06605 (L2Y96_06605) - 1477325..1478335 (-) 1011 WP_247334310.1 glycosyltransferase -
  L2Y96_RS06610 (L2Y96_06610) - 1478332..1479237 (-) 906 WP_247334312.1 NAD-dependent epimerase/dehydratase family protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62504.19 Da        Isoelectric Point: 6.6450

>NTDB_id=677976 L2Y96_RS06585 WP_247334303.1 1472856..1474583(-) (pilB) [Luteibacter aegosomaticola strain 335]
MATQMHQPTLAGLTGMARRLVTEGVLGEADVRKAVQDAADKRTSLSAWLVDHHLVDSAKLSQVASAEFGMPLMDIGNMVP
GNMPLDLVTEALITKHQALPLFKRGKRLFVGIADPMQSHALDEIKFHSNHMVEPVLVERGQLRRIIDSALSAANAGVPAF
DDSALDELALDAGPDDGDGTTGIDANANDDAPIVKFVNKILVDAIKRGASDIHFEPFETVYRVRLRMDGILRIVASAPIK
LGTRIASRLKVMSGLDIAERRVPQDGRIKLNLTKTRAIDFRVSTLPTLFGEKIVLRVLDGSSAKLGIDKLGYEEVQKNLY
LDAIEKPYGMVLVTGPTGSGKTVSLYTALNILNTEGRNISTVEDPVEIRVEGINQVQQNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNIISSVTLIIAQRLARRLHDCKREIHLPPAA
LLAEGYTQEEIDAGITIYEAVGCDGCNEGYKGRVGLYQVMPMLEPIQKIVLEGGNAMQIAEVSRKAGINDLRASALLKVR
NGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=677976 L2Y96_RS06585 WP_247334303.1 1472856..1474583(-) (pilB) [Luteibacter aegosomaticola strain 335]
ATGGCGACACAAATGCACCAGCCTACGCTTGCGGGCCTGACTGGCATGGCCAGGCGCCTCGTCACGGAAGGCGTCCTTGG
TGAGGCCGATGTCCGCAAGGCCGTCCAGGACGCCGCCGATAAGCGAACCTCGCTGTCCGCATGGCTGGTGGACCACCACC
TGGTCGACAGCGCCAAGCTCTCCCAGGTGGCCTCGGCCGAGTTCGGCATGCCGCTTATGGATATCGGCAACATGGTCCCC
GGCAACATGCCGCTGGACCTGGTCACCGAAGCCCTGATCACGAAGCACCAGGCCCTGCCCCTGTTCAAGCGCGGCAAGCG
CCTGTTCGTGGGCATCGCCGACCCGATGCAGTCGCACGCGCTGGACGAGATCAAGTTCCACTCCAACCACATGGTGGAGC
CGGTGCTGGTCGAACGTGGCCAGTTGCGCCGGATCATCGATAGCGCCCTGTCGGCCGCCAATGCCGGCGTCCCGGCCTTC
GACGACTCCGCCCTCGACGAACTGGCGCTCGACGCCGGCCCGGATGACGGCGACGGCACCACCGGCATCGACGCCAATGC
GAACGATGACGCTCCGATCGTCAAGTTCGTGAACAAGATCCTGGTGGATGCCATCAAGCGCGGCGCCTCGGATATCCACT
TCGAGCCGTTTGAAACGGTTTACCGGGTCCGCCTGCGCATGGACGGCATCCTGCGAATCGTGGCCTCGGCCCCGATCAAA
CTGGGCACCCGTATCGCCTCGCGCCTGAAGGTCATGAGCGGCCTGGACATCGCCGAACGCCGTGTCCCGCAGGACGGCCG
TATCAAGCTCAACCTCACCAAGACCCGCGCCATCGACTTCCGCGTCAGCACCCTGCCCACCCTGTTCGGTGAAAAGATCG
TGCTCCGCGTGCTCGATGGCTCCTCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGGAGGTCCAGAAGAACCTCTAC
CTCGACGCCATCGAGAAGCCGTACGGCATGGTGCTGGTCACCGGTCCCACCGGTTCGGGTAAGACGGTGTCGTTGTACAC
GGCGCTGAACATCCTCAACACCGAGGGCAGAAACATCTCCACGGTGGAAGACCCGGTCGAAATCCGCGTCGAAGGCATCA
ACCAGGTCCAGCAGAACGTGAAGCGCGGCATGACCTTCGCCGCCGCCCTGCGCTCCTTCCTGCGCCAGGATCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTCGAGACCGCCGAAATCGCCGTGAAGGCCGCCCAGACCGGCCACATGGTGCTCTC
CACCCTGCATACCAACGATGCGCCGCAAACCATTGCGCGCCTGATGAATATGGGCATCGCGCCCTACAACATCATTTCGT
CGGTCACCCTGATCATCGCCCAGCGCCTGGCCCGCCGCCTGCACGACTGCAAGCGCGAGATCCACCTGCCCCCCGCGGCC
CTGCTGGCCGAGGGGTACACCCAGGAGGAGATCGACGCCGGTATCACGATTTACGAGGCGGTAGGTTGCGATGGCTGCAA
CGAGGGCTACAAGGGGCGCGTCGGCCTCTACCAGGTCATGCCCATGCTGGAGCCGATCCAGAAGATCGTCCTGGAGGGTG
GCAACGCCATGCAGATCGCCGAGGTGTCACGGAAAGCCGGTATCAACGACCTGCGAGCCTCGGCCCTGCTGAAGGTCAGG
AACGGCGTGACCAGCCTGGCCGAAATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.386

99.13

0.539
  pilB Acinetobacter baylyi ADP1

53.982

98.261

0.53
  pilB Legionella pneumophila strain ERS1305867

50.617

98.609

0.499
  pilB Vibrio cholerae strain A1552

48.845

97.913

0.478
  pilB Vibrio campbellii strain DS40M4

46.127

98.783

0.456
  pilB Vibrio parahaemolyticus RIMD 2210633

46.359

97.913

0.454
  pilF Neisseria gonorrhoeae MS11

48.859

91.478

0.447
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.088

100

0.372