Detailed information NTG0677964

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: L2Y94_RS16055
• Genome accession: NZ_CP095740
• Coordinates: 3570068..3571804 (+)
• Length: 578 a.a.
• NCBI ID: WP_247368919.1
• Uniprot ID: -
• Organism: Luteibacter aegosomatis strain 321
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3550877..3569888	3570068..3571804	flank	180

Gene organization within MGE regions

Location: 3550877..3571804

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L2Y94_RS15975 (L2Y94_15975)	-	3550877..3552802 (+)	1926	WP_247368831.1	hypothetical protein	-
L2Y94_RS15980 (L2Y94_15980)	-	3552885..3554966 (-)	2082	WP_247368863.1	S9 family peptidase	-
L2Y94_RS15985 (L2Y94_15985)	pilA2	3555384..3555800 (+)	417	WP_247368866.1	pilin	Machinery gene
L2Y94_RS15990 (L2Y94_15990)	-	3555873..3557819 (+)	1947	WP_247368869.1	hypothetical protein	-
L2Y94_RS16000 (L2Y94_16000)	-	3557939..3558379 (+)	441	WP_283248495.1	pilin	-
L2Y94_RS16005 (L2Y94_16005)	-	3558547..3560454 (+)	1908	WP_247368871.1	hypothetical protein	-
L2Y94_RS16010 (L2Y94_16010)	-	3560454..3561701 (+)	1248	WP_247368889.1	oligosaccharide flippase family protein	-
L2Y94_RS16015 (L2Y94_16015)	-	3561657..3562547 (-)	891	WP_247368892.1	glycosyltransferase family 2 protein	-
L2Y94_RS16020 (L2Y94_16020)	-	3562544..3563347 (-)	804	WP_247368894.1	class I SAM-dependent methyltransferase	-
L2Y94_RS16025 (L2Y94_16025)	-	3563572..3564564 (-)	993	WP_247368897.1	glycosyltransferase family 4 protein	-
L2Y94_RS16030 (L2Y94_16030)	-	3564591..3565748 (-)	1158	WP_247368900.1	glycosyltransferase family 4 protein	-
L2Y94_RS16035 (L2Y94_16035)	-	3565759..3566481 (-)	723	WP_247368911.1	class I SAM-dependent methyltransferase	-
L2Y94_RS16040 (L2Y94_16040)	-	3566478..3567350 (-)	873	WP_247368914.1	glycosyltransferase family 2 protein	-
L2Y94_RS16045 (L2Y94_16045)	-	3567347..3568516 (-)	1170	WP_247368916.1	hypothetical protein	-
L2Y94_RS16050 (L2Y94_16050)	-	3568491..3569888 (-)	1398	WP_247375289.1	MBOAT family O-acyltransferase	-
L2Y94_RS16055 (L2Y94_16055)	pilB	3570068..3571804 (+)	1737	WP_247368919.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 578 a.a.
• Molecular weight: 62799.42 Da
• Isoelectric Point: 6.2101

>NTDB_id=677964 L2Y94_RS16055 WP_247368919.1 3570068..3571804(+) (pilB) [Luteibacter aegosomatis strain 321]
MVSNMQQPMLAGLTGMARRLVTEGVLPEADVRKAVQDAAEKRVSLSAWLVDHNLVDSGRLSQVASAEFGMPLMDLSAMAP
AGMPLDLVTEALISKHQALPLFKRGKRLFVGIADPMQSHALDEIKFHSNHMVEPVLVERAQLRRVIDSALSTMSSTVPGF
GDDGGLEELSLESGDDDADGGASTGIDANANDDAPVVKFVNKILVDAIKRGASDIHFEPFESVYRVRLRMDGILRIVANA
PIKLGTRIASRLKVMSGLDIAERRVPQDGRIKLNLTKTRAIDFRVSTLPTLFGEKIVLRILDGSSAKLGIDKLGYEEDQK
KLYLDAIEKPYGMVLVTGPTGSGKTVSLYTALNILNVEGRNISTVEDPVEIRVEGINQVQQNTKRGMTFAAALRSFLRQD
PDVIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLIIAQRLARRLHDCKRQVHLP
PAALLAEGFTQEEIDAGMTVYEPVGCDGCNEGYKGRVGIYQVMPMVEEIQKIVLEGGNALQIAEVATRAGINDLRASALL
KVRNGVTSLAEINRVTKD

Nucleotide

• Length: 1737 bp

>NTDB_id=677964 L2Y94_RS16055 WP_247368919.1 3570068..3571804(+) (pilB) [Luteibacter aegosomatis strain 321]
ATGGTTTCGAACATGCAGCAACCCATGCTCGCCGGCCTGACGGGCATGGCACGGCGATTGGTCACCGAGGGCGTGCTGCC
CGAGGCCGACGTGCGCAAGGCGGTGCAGGACGCCGCCGAGAAGCGCGTTTCCCTCTCGGCCTGGCTGGTCGACCACAACC
TCGTGGACAGCGGCCGCCTCTCCCAGGTGGCCTCGGCCGAATTCGGCATGCCGCTGATGGACCTCAGCGCCATGGCGCCT
GCCGGCATGCCACTGGACCTGGTTACCGAGGCCCTGATCTCCAAGCACCAGGCCCTGCCCCTGTTCAAGCGCGGCAAGCG
CCTGTTCGTGGGCATCGCCGATCCCATGCAGTCCCACGCGCTGGACGAGATCAAGTTCCACTCCAACCACATGGTGGAGC
CGGTGCTGGTGGAGCGCGCGCAACTGCGCCGAGTGATCGATTCGGCCCTGTCCACCATGAGCAGCACGGTGCCGGGCTTC
GGCGACGACGGCGGCCTGGAAGAACTCTCGCTGGAAAGCGGCGACGACGATGCCGACGGCGGCGCCAGCACGGGCATCGA
CGCCAACGCCAACGACGACGCCCCGGTCGTCAAGTTCGTCAACAAGATCCTGGTCGACGCCATCAAGCGCGGCGCCTCCG
ACATCCATTTCGAGCCGTTCGAGTCGGTCTACCGCGTGCGCCTGCGCATGGACGGCATCCTGCGCATCGTGGCCAACGCC
CCCATCAAGCTCGGCACGCGCATCGCCTCGCGCCTGAAGGTGATGAGCGGCCTGGACATCGCCGAACGCCGCGTGCCGCA
GGACGGCCGCATCAAGCTCAACCTCACCAAGACCCGCGCCATCGACTTCCGCGTGAGCACCCTGCCCACGCTGTTCGGCG
AGAAGATCGTGCTGCGTATCCTCGACGGCTCGTCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGGAAGACCAGAAG
AAGCTCTACCTCGACGCCATCGAAAAACCCTACGGCATGGTGCTGGTCACCGGCCCCACGGGCTCGGGTAAAACCGTCTC
GCTCTACACCGCCCTTAACATCCTCAACGTCGAGGGACGCAACATCTCCACCGTGGAAGACCCCGTGGAAATCCGCGTCG
AGGGCATCAACCAGGTGCAGCAGAACACCAAGCGCGGCATGACCTTCGCGGCTGCCCTCCGCTCCTTCCTGCGCCAGGAT
CCGGACGTGATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCCATCAAGGCCGCGCAGACCGGCCATAT
GGTGCTGTCCACCCTGCACACCAACGACGCGCCGCAGACCATCGCCCGCCTGATGAACATGGGCATCGCGCCCTACAACA
TCACCTCGTCGGTCACCCTGATCATCGCCCAGCGCCTGGCCCGCCGCCTGCACGACTGCAAGCGCCAGGTGCACCTGCCG
CCCGCCGCCCTGCTCGCCGAGGGGTTCACCCAGGAGGAGATCGACGCCGGCATGACGGTCTACGAGCCGGTGGGTTGCGA
CGGCTGCAACGAGGGCTACAAGGGCCGCGTGGGCATCTACCAGGTGATGCCGATGGTCGAGGAAATCCAGAAGATCGTGC
TGGAGGGCGGCAACGCCCTGCAGATCGCCGAGGTGGCCACCCGGGCGGGCATCAACGACCTGCGCGCCTCGGCCCTGCTC
AAGGTCCGCAACGGCGTGACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (20-153)

• Type II/IV secretion system protein (T2SSE) (202-469)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	56.14	98.616	0.554
pilB	Acinetobacter baylyi ADP1	54.754	98.27	0.538
pilB	Legionella pneumophila strain ERS1305867	50.175	98.962	0.497
pilB	Vibrio parahaemolyticus RIMD 2210633	47.872	97.578	0.467
pilB	Vibrio cholerae strain A1552	47.957	97.405	0.467
pilB	Vibrio campbellii strain DS40M4	46.41	98.789	0.458
pilF	Neisseria gonorrhoeae MS11	49.064	92.388	0.453
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	36.471	100	0.375
pilF	Thermus thermophilus HB27	37.611	97.059	0.365