Detailed information NTG0676767

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MWH18_RS15365
• Genome accession: NZ_CP095407
• Coordinates: 3136025..3137143 (+)
• Length: 372 a.a.
• NCBI ID: WP_032057670.1
• Uniprot ID: -
• Organism: Acinetobacter pittii strain TCM
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3131025..3142143

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MWH18_RS15355 (MWH18_15355)	yggS	3134149..3134841 (-)	693	WP_032057669.1	YggS family pyridoxal phosphate-dependent enzyme	-
MWH18_RS15360 (MWH18_15360)	pilT	3134968..3136005 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
MWH18_RS15365 (MWH18_15365)	pilU	3136025..3137143 (+)	1119	WP_032057670.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MWH18_RS15370 (MWH18_15370)	fur	3137213..3137650 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
MWH18_RS15375 (MWH18_15375)	omlA	3137762..3138160 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
MWH18_RS15380 (MWH18_15380)	-	3138225..3138539 (-)	315	WP_017481335.1	RnfH family protein	-
MWH18_RS15385 (MWH18_15385)	-	3138539..3139606 (-)	1068	WP_031944146.1	membrane protein	-
MWH18_RS15390 (MWH18_15390)	-	3139760..3140215 (-)	456	WP_002121698.1	bacteriohemerythrin	-
MWH18_RS15395 (MWH18_15395)	-	3140467..3141315 (-)	849	WP_014205980.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41975.16 Da
• Isoelectric Point: 6.5062

>NTDB_id=676767 MWH18_RS15365 WP_032057670.1 3136025..3137143(+) (pilU) [Acinetobacter pittii strain TCM]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEDPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=676767 MWH18_RS15365 WP_032057670.1 3136025..3137143(+) (pilU) [Acinetobacter pittii strain TCM]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAATTTTGCGATTATGAACCGCGATAAAAGTGCG
CGCTTTCGTGTCAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTATTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAATTACCACCTGTGCTTAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCTTTGGCTTCAATGATTAGCTATCGGAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGTTGCATCATTACCCAACGTGAAGTCGGGATTGATACAGACTCATTCGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCTCGCCGTGCAGCGATTGAGATTCTCATTAACTCCCCATTAATTTCAGACT
ATATTCGTAAAGGTGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTCGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGATCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.003	99.731	0.688
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363