Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MUU73_RS04950 Genome accession   NZ_CP095184
Coordinates   1034315..1036057 (+) Length   580 a.a.
NCBI ID   WP_245125214.1    Uniprot ID   -
Organism   Pseudoxanthomonas mexicana strain F12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1029315..1041057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUU73_RS04930 (MUU73_04930) - 1029711..1031804 (+) 2094 WP_245125203.1 tetratricopeptide repeat protein -
  MUU73_RS04935 (MUU73_04935) - 1031686..1032660 (+) 975 WP_245125206.1 glycosyltransferase family 2 protein -
  MUU73_RS04940 (MUU73_04940) - 1032657..1033265 (+) 609 WP_245125209.1 methyltransferase domain-containing protein -
  MUU73_RS04945 (MUU73_04945) - 1033262..1034164 (+) 903 WP_245125212.1 lysylphosphatidylglycerol synthase domain-containing protein -
  MUU73_RS04950 (MUU73_04950) pilB 1034315..1036057 (+) 1743 WP_245125214.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MUU73_RS04955 (MUU73_04955) pilC 1036279..1037535 (+) 1257 WP_245125217.1 type II secretion system F family protein Machinery gene
  MUU73_RS04960 (MUU73_04960) pilD 1037545..1038408 (+) 864 WP_245125220.1 A24 family peptidase Machinery gene
  MUU73_RS04965 (MUU73_04965) coaE 1038442..1039059 (+) 618 WP_245125223.1 dephospho-CoA kinase -
  MUU73_RS04970 (MUU73_04970) - 1039759..1040184 (-) 426 WP_245125225.1 transposase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62824.27 Da        Isoelectric Point: 5.9546

>NTDB_id=675980 MUU73_RS04950 WP_245125214.1 1034315..1036057(+) (pilB) [Pseudoxanthomonas mexicana strain F12]
MNAVASPTANLVGITGIARRLVLDGAMDEAAARSAMANATEAKMPLAQWVAEKRLVSAAHLAAANAIEFGMPLIDVTTFD
PAHHAVKLVSEELIQKHQVLPLFKRGGKLFVGTSNPTQTAALTEIQFHTNLVVEPILVDEDQLRRTLDQWLNAANNLSGS
FGGDDEEGLDNLEVGTDEDMGGGADSAVDAKGDDTPVVKFVNKVLVDAIKRGASDIHFEPYETDYRVRLRIDGILKQVAK
APVKLSSRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPDQ
QKLFEEAIQKPYGMVLVTGPTGSGKTVSLYTALGILNDEVRNISTVEDPVEIRLPGVNQVQQNTKRGMTFAAALRSFLRQ
DPDIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCPKCKKPVE
LPKPALLAEGFTEAEIDAGFTLYDAVGCDECTEGYKGRTGIYQVMPMSDDIQAIVLEGGNAMQIAAVAQKAGIRDLRQSA
LMKVRNGVTSLAEINRVTKD

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=675980 MUU73_RS04950 WP_245125214.1 1034315..1036057(+) (pilB) [Pseudoxanthomonas mexicana strain F12]
ATGAACGCAGTCGCCAGCCCCACCGCCAATCTCGTCGGCATCACCGGCATTGCCCGCCGCCTGGTCTTGGACGGGGCGAT
GGACGAAGCGGCCGCGCGCAGTGCGATGGCGAATGCCACGGAAGCGAAGATGCCGTTGGCCCAATGGGTGGCGGAAAAGC
GTCTGGTCAGCGCGGCACACCTCGCCGCCGCCAACGCCATCGAGTTCGGCATGCCGCTGATCGATGTCACCACCTTCGAC
CCCGCCCACCACGCCGTCAAGCTGGTCAGCGAAGAGTTGATCCAGAAGCACCAGGTACTGCCGCTTTTCAAGCGCGGCGG
CAAGCTGTTCGTCGGCACCAGCAATCCCACCCAGACCGCGGCCCTGACCGAGATCCAGTTCCACACCAACCTGGTGGTGG
AGCCCATTCTGGTCGATGAGGACCAGCTACGCCGTACCCTAGATCAGTGGCTCAACGCCGCCAACAACCTGTCTGGCAGC
TTCGGCGGCGACGACGAAGAGGGGCTGGACAACCTGGAGGTGGGTACCGACGAGGACATGGGCGGTGGCGCCGACAGCGC
CGTCGACGCCAAGGGCGACGACACCCCGGTGGTCAAGTTCGTCAACAAGGTGCTGGTGGACGCCATCAAGCGCGGCGCCT
CGGACATCCACTTCGAGCCCTACGAAACCGACTACCGCGTGCGCCTGCGCATCGACGGCATCCTCAAGCAGGTCGCCAAG
GCACCGGTGAAGCTGAGCTCGCGCATCGCCGCACGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAACGCGTCCC
GCAGGACGGCCGCATCAAGCTGAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCCACGCTGTTCG
GCGAAAAGATCGTGCTGCGCATCCTGGACGGCAGCGCCGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAG
CAGAAGCTGTTCGAAGAAGCCATCCAGAAGCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGT
GTCGCTGTACACCGCACTGGGCATCCTGAACGACGAGGTGCGCAACATCTCCACCGTCGAGGACCCGGTGGAAATCCGCC
TGCCCGGCGTGAACCAGGTGCAGCAGAACACCAAGCGCGGCATGACCTTCGCCGCCGCCCTGCGCAGCTTCCTGCGCCAG
GACCCGGACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCA
CATGGTGCTGTCCACCCTGCATACCAACGACGCTCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCCCCGTACA
ACATCACCAGCTCGGTAACCCTGGTGATCGCCCAGCGCCTGGCGCGGCGGCTGTGCCCCAAGTGCAAGAAGCCGGTCGAA
CTGCCGAAGCCCGCCCTGTTGGCCGAAGGCTTCACCGAGGCCGAGATCGACGCCGGCTTCACCCTGTACGACGCGGTCGG
CTGCGACGAATGCACCGAGGGCTACAAGGGCCGGACCGGCATCTACCAGGTCATGCCGATGTCCGACGACATCCAGGCCA
TCGTGCTGGAAGGCGGCAACGCCATGCAGATCGCCGCTGTCGCCCAGAAGGCCGGCATCCGCGACCTGCGCCAGTCCGCG
CTGATGAAGGTCCGCAACGGCGTCACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.316

98.276

0.553
  pilB Acinetobacter baylyi ADP1

55.077

100

0.552
  pilB Legionella pneumophila strain ERS1305867

52.817

97.931

0.517
  pilB Vibrio cholerae strain A1552

48.793

100

0.488
  pilF Neisseria gonorrhoeae MS11

51.224

91.552

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

49.057

91.379

0.448
  pilB Vibrio campbellii strain DS40M4

48.473

90.345

0.438