Detailed information NTG0674632

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MTZ49_RS09680
• Genome accession: NZ_CP094972
• Coordinates: 2014218..2015351 (+)
• Length: 377 a.a.
• NCBI ID: WP_264745350.1
• Uniprot ID: -
• Organism: Entomomonas sp. E2T0
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2009218..2020351

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MTZ49_RS09650 (MTZ49_09640)	-	2009366..2010811 (+)	1446	WP_264745344.1	hypothetical protein	-
MTZ49_RS09655 (MTZ49_09645)	-	2011335..2012003 (+)	669	WP_264745345.1	hypothetical protein	-
MTZ49_RS09660 (MTZ49_09650)	-	2012012..2012533 (+)	522	WP_264745346.1	GTP-binding protein	-
MTZ49_RS09665 (MTZ49_09655)	-	2012565..2012993 (+)	429	WP_264745347.1	roadblock/LC7 domain-containing protein	-
MTZ49_RS09670 (MTZ49_09660)	-	2013020..2013382 (+)	363	WP_264745348.1	hypothetical protein	-
MTZ49_RS09675 (MTZ49_09665)	-	2013539..2013988 (+)	450	WP_264745349.1	CZB domain-containing protein	-
MTZ49_RS09680 (MTZ49_09670)	pilU	2014218..2015351 (+)	1134	WP_264745350.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MTZ49_RS09685 (MTZ49_09675)	-	2015797..2016678 (+)	882	WP_264745351.1	DNA adenine methylase	-
MTZ49_RS09690 (MTZ49_09680)	aceK	2016843..2018558 (-)	1716	WP_264745352.1	bifunctional isocitrate dehydrogenase kinase/phosphatase	-
MTZ49_RS09695 (MTZ49_09685)	aceA	2018649..2019956 (-)	1308	WP_264745353.1	isocitrate lyase	-

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 42071.86 Da
• Isoelectric Point: 6.6124

>NTDB_id=674632 MTZ49_RS09680 WP_264745350.1 2014218..2015351(+) (pilU) [Entomomonas sp. E2T0]
MEVEKLLKFMVDKKASDMFLTVGIPPTIKVDGRLMPLPVNKLTNDVLKKLVRSIMTERLQKEFAATQECNFAVSYPNVGR
FRVSAYIQRSMVGMVLRRIVTDIPSIDNLQLPKVVKELAMVKRGLVLFVGATGAGKSTSLAAMVDYRNTHSTGHIISIED
PVEYLFANKNCIVTQREVGLDTESFEVALKNTLRQAPDVIMIGEVRTRQTMEYALAFAETGHLCLATLHANNANQALDRI
ISFFATERHAQVMLDLSLNLRAIVAQQLVADKEEKGRHAAIEVLLNTPIVSDMIRTGQVTELKGIMSRSVELGMQTFDQS
LFDLFERGLISYESALACADSANDLRLMIKLKSEQNIAGTKQLVGMQLEDYEEEHYG

Nucleotide

• Length: 1134 bp

>NTDB_id=674632 MTZ49_RS09680 WP_264745350.1 2014218..2015351(+) (pilU) [Entomomonas sp. E2T0]
ATGGAAGTTGAGAAATTATTAAAGTTTATGGTGGATAAAAAAGCGTCTGATATGTTTTTAACCGTTGGAATTCCACCTAC
TATAAAGGTTGATGGGAGATTAATGCCATTACCTGTAAACAAATTAACAAATGATGTATTAAAAAAACTTGTGCGCTCAA
TCATGACAGAGCGTTTACAAAAAGAATTTGCTGCAACACAAGAATGTAATTTTGCAGTGAGCTATCCAAATGTAGGGCGA
TTTCGTGTAAGTGCTTATATACAAAGAAGTATGGTGGGTATGGTATTAAGGCGCATTGTTACGGATATACCTAGTATTGA
TAATTTGCAGTTACCTAAAGTTGTTAAAGAGTTGGCTATGGTTAAACGTGGCCTAGTACTGTTTGTGGGGGCAACAGGTG
CAGGTAAGTCAACTTCTTTAGCAGCTATGGTGGATTATCGTAATACTCACTCAACAGGGCATATTATTTCTATTGAAGAT
CCTGTGGAGTACTTATTTGCTAATAAAAATTGTATTGTTACTCAACGTGAGGTGGGGCTAGATACTGAGTCATTTGAAGT
AGCATTAAAAAACACGCTTCGGCAGGCTCCCGACGTTATTATGATCGGTGAGGTGCGTACGCGGCAAACAATGGAATATG
CATTAGCATTTGCTGAAACAGGGCATCTCTGTTTGGCTACACTCCATGCTAATAATGCTAATCAGGCATTGGATAGAATT
ATTAGTTTCTTTGCAACGGAACGTCATGCACAAGTTATGTTAGACCTTTCTTTAAATCTACGAGCCATTGTTGCACAACA
ATTAGTTGCAGATAAAGAAGAAAAGGGCAGACATGCGGCTATAGAAGTATTACTTAATACACCTATTGTTTCAGATATGA
TTCGTACTGGGCAAGTAACAGAGCTTAAAGGTATTATGTCTCGTTCAGTTGAGTTGGGTATGCAAACATTTGATCAGTCA
CTGTTTGATTTATTTGAGCGAGGACTAATTAGCTATGAGTCTGCATTGGCTTGTGCCGATTCGGCCAATGATTTGAGGTT
AATGATTAAGTTAAAGTCAGAACAAAATATTGCTGGCACAAAACAATTGGTTGGTATGCAACTTGAAGATTATGAAGAAG
AACATTATGGATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	62.198	98.939	0.615
pilU	Vibrio cholerae strain A1552	55.946	98.143	0.549
pilU	Acinetobacter baylyi ADP1	57.983	94.695	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.888	89.92	0.377
pilT	Legionella pneumophila strain Lp02	40.118	89.92	0.361
pilT	Legionella pneumophila strain ERS1305867	40.118	89.92	0.361