Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LEN_RS18510 Genome accession   NZ_AP014940
Coordinates   4618720..4619886 (-) Length   388 a.a.
NCBI ID   WP_096379613.1    Uniprot ID   A0AAU9AQM2
Organism   Lysobacter enzymogenes strain M497-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4613720..4624886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEN_RS18510 (LEN_3748) pilU 4618720..4619886 (-) 1167 WP_096379613.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LEN_RS18515 (LEN_3749) pilT 4620002..4621039 (-) 1038 WP_074864167.1 type IV pilus twitching motility protein PilT Machinery gene
  LEN_RS18520 (LEN_3750) - 4621274..4621936 (+) 663 WP_232518547.1 YggS family pyridoxal phosphate-dependent enzyme -
  LEN_RS18525 (LEN_3751) - 4621933..4622490 (+) 558 WP_096379616.1 hypothetical protein -
  LEN_RS18530 (LEN_3752) proC 4622514..4623356 (+) 843 WP_096379618.1 pyrroline-5-carboxylate reductase -
  LEN_RS18535 (LEN_3753) - 4623353..4623835 (+) 483 WP_096379620.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43087.54 Da        Isoelectric Point: 6.8285

>NTDB_id=67458 LEN_RS18510 WP_096379613.1 4618720..4619886(-) (pilU) [Lysobacter enzymogenes strain M497-1]
MNTPAPTNPTTASGAIDFTSFLKLMAHQRASDLFITAGMPPSMKVHGKIAPITQNPLTPQQSRDLVLNVMNPQQREEFEK
THECNFAIGVTGVGRFRVSCFYQRNQVGMVLRRIETKIPTVEELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIG
YRNQNSTGHIITIEDPIEFVHRHEGCIITQREVGIDTDSWDAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVL
CTLHANNANQAMDRIINFFPEDRRLQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLAQDYIRDGEIHKLKEL
MKDSVQLGMKTFDQALFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLSQGLDGVEVAEVR

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=67458 LEN_RS18510 WP_096379613.1 4618720..4619886(-) (pilU) [Lysobacter enzymogenes strain M497-1]
ATGAACACGCCCGCTCCGACCAATCCGACCACCGCCTCCGGCGCGATCGATTTCACCTCCTTCCTCAAGCTGATGGCGCA
CCAGCGCGCCTCCGACTTGTTCATCACCGCCGGCATGCCGCCGTCGATGAAGGTGCACGGCAAGATCGCGCCGATCACCC
AGAACCCGCTGACGCCGCAGCAAAGCCGCGACCTCGTGCTGAACGTGATGAACCCGCAGCAGCGCGAAGAGTTCGAAAAG
ACCCACGAGTGCAACTTCGCCATCGGCGTCACCGGCGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGT
CGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCCGACCGTCGAAGAGCTGAACCTGCCGCCGATCATCAAGACGCTGG
CGATGACCAAGCGCGGCATCATCATCTTCGTCGGCGCCACCGGCACCGGCAAATCGACCTCGCTGGCGGCGATGATCGGC
TACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAGGACCCGATCGAGTTCGTGCACCGCCACGAGGGCTGCAT
CATCACCCAGCGCGAAGTCGGCATCGACACCGACAGCTGGGACGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACG
TGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTG
TGCACGCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCCTGCAGCT
GCTGATGGACTTGTCGCTGAACCTCAAGGGCGTGGTCGCCCAGCAGCTGATCCCGACTCCCGACGGCAAGGGCCGCCGCG
TAGCGATGGAGATCCTGCTCGGCACCCCGCTGGCGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAAGAGCTG
ATGAAGGACTCCGTGCAGCTCGGCATGAAGACCTTCGACCAGGCCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTA
TGAAGACGCGCTGCGCTACGCCGACTCGCAGAACGAGGTGCGCCTGCGGATCAAGCTGGCGCAGGGCGGGGATGCGCGGA
CCTTGTCGCAGGGGTTGGATGGGGTGGAGGTGGCCGAGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.21

94.588

0.655
  pilU Acinetobacter baylyi ADP1

64.426

92.01

0.593
  pilU Vibrio cholerae strain A1552

53.371

91.753

0.49
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.45

90.464

0.384

Multiple sequence alignment