Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MON37_RS09065 Genome accession   NZ_CP094242
Coordinates   1919065..1920744 (-) Length   559 a.a.
NCBI ID   WP_039405482.1    Uniprot ID   A0AAW6ZCC1
Organism   Morococcus cerebrosus strain CIP 81.93     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1914065..1925744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MON37_RS09035 (MON37_09035) ribF 1914554..1915483 (+) 930 WP_039405471.1 bifunctional riboflavin kinase/FAD synthetase -
  MON37_RS09040 (MON37_09040) yacG 1915585..1915779 (-) 195 WP_070440456.1 DNA gyrase inhibitor YacG -
  MON37_RS09045 (MON37_09045) - 1915818..1916099 (-) 282 WP_082013583.1 hypothetical protein -
  MON37_RS09050 (MON37_09050) coaE 1916256..1916885 (-) 630 WP_039405473.1 dephospho-CoA kinase -
  MON37_RS09055 (MON37_09055) pilD 1916887..1917750 (-) 864 WP_039405476.1 A24 family peptidase Machinery gene
  MON37_RS09060 (MON37_09060) pilG 1917752..1918993 (-) 1242 WP_039405479.1 type II secretion system F family protein Machinery gene
  MON37_RS09065 (MON37_09065) pilF 1919065..1920744 (-) 1680 WP_039405482.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MON37_RS09070 (MON37_09070) bamE 1921024..1921491 (-) 468 WP_039405485.1 outer membrane protein assembly factor BamE -
  MON37_RS09075 (MON37_09075) - 1921559..1922656 (-) 1098 WP_039405488.1 YadA-like family protein -
  MON37_RS09080 (MON37_09080) waaA 1923480..1924742 (+) 1263 WP_039405492.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  MON37_RS09085 (MON37_09085) - 1924997..1925218 (-) 222 WP_003741122.1 glycine zipper 2TM domain-containing protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62435.02 Da        Isoelectric Point: 6.3585

>NTDB_id=668360 MON37_RS09065 WP_039405482.1 1919065..1920744(-) (pilF) [Morococcus cerebrosus strain CIP 81.93]
MSIGLLRVLVQSQTINNTQAEHYNNILKTGQEILPMLFADKIISPRSLGELVARVFSYPLLDLHYYPRNNIVMDVLTEEQ
MVQNRCVPIFRRGRKVYLAVSDPTQIQSFQKIAFASGVTVDLVVVPDDQLSSLLEWLGQRSTTILKEISEEQEAAQPSQA
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAATLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEDVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKREVERPPVPALKKAGFTDEDLAKDWKL
YRPVGCDSCRGKGFKGRVGVYEVMPITEEMQRVIMNNGTEVDIMNMAYKEGMVDLRRAGLLKVMQGLTSLEEVIAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=668360 MON37_RS09065 WP_039405482.1 1919065..1920744(-) (pilF) [Morococcus cerebrosus strain CIP 81.93]
ATGAGTATCGGATTATTACGAGTTTTGGTTCAAAGCCAGACAATCAACAATACCCAGGCAGAGCATTACAATAATATATT
GAAAACGGGGCAGGAAATCTTGCCCATGCTTTTTGCGGATAAGATTATCAGCCCAAGGTCTTTGGGCGAGTTGGTTGCGC
GAGTTTTTAGTTATCCGCTATTGGATTTGCATTATTATCCACGTAACAATATTGTTATGGATGTCTTAACTGAGGAGCAG
ATGGTTCAAAATCGCTGCGTTCCGATTTTTCGTCGCGGCCGAAAGGTATATTTGGCGGTTTCAGATCCTACTCAAATCCA
AAGTTTTCAAAAAATAGCTTTTGCTTCGGGAGTGACGGTTGATTTAGTAGTTGTCCCGGATGATCAGTTGAGTTCTTTGC
TGGAGTGGTTAGGACAACGTTCTACCACCATCTTAAAAGAAATTAGTGAAGAACAAGAAGCTGCTCAGCCCTCTCAGGCG
CTTTATATTGACAACGAAGAGGCTGAAGATGGGCCTATTCCACGCTTTATTCACAAAACTTTATCAGATGCACTAAATGC
AGGCGCATCCGATATACATTTCGAATTTTATGAACAAATGGCTCGTGTCCGTTTTCGTGTAGATGGACAGCTAAGGGAAG
TGGTTCAACCTCCTGTTGCTGTACGCGGACAGCTGGCTTCACGCATCAAAGTAATGGCGCGTTTGGACATTTCGGAAAAA
CGCGTACCGCAAGATGGTCGTATTCAGATTGCTTTCCATAAACACGGTCGCCCTATTGATTTTCGTGTAAGTACGTTACC
TACGCTTTTTGGCGAAAAAGTGGTAATGCGTATTCTGAATTCTGATGCAGCAACTTTAAATATTGACCAACTGGGATTTG
AACCCTTCCAAAAAGAAATGCTGCTTGAGGCCATTCATCGGCCTTATGGCATGGTATTGGTAACAGGTCCGACAGGCTCC
GGTAAAACCGTATCACTTTATACTTGCTTGAATATTTTAAATACAGAAGACGTCAATATTTCCACCGCAGAAGACCCGGC
CGAGATTAATTTGCCCGGCATCAACCAAGTCAACGTCAATGACAAACAAGGTTTGACTTTTGCTGCGGCGTTGAAATCTT
TCTTACGACAAGACCCTGACATTATCATGGTAGGTGAGATTCGGGATTTGGAAACCGCGGATATTGCCATCAAAGCGGCA
CAAACCGGACATATGGTGTTTTCAACGTTGCATACCAATAACGCGCCTGCGACCCTATCCCGTATGTTGAACATGGGGGT
TGCCCCATTCAACATTGCCAGTTCCGTCAGCTTAATTATGGCGCAACGACTATTGCGTCGTCTGTGTTCGAGCTGTAAGC
GTGAGGTTGAGCGTCCTCCAGTTCCCGCATTAAAAAAAGCAGGATTTACAGATGAAGATTTAGCAAAGGATTGGAAACTC
TACCGTCCTGTCGGTTGCGATAGTTGCCGTGGTAAAGGCTTTAAAGGCCGTGTCGGTGTCTACGAAGTAATGCCGATTAC
CGAGGAAATGCAACGTGTCATTATGAATAATGGTACGGAAGTTGATATTATGAACATGGCCTATAAAGAAGGCATGGTCG
ATTTACGCCGTGCAGGCTTATTAAAAGTTATGCAAGGTTTGACTTCATTAGAAGAAGTTATTGCTCACACTAACGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

80.322

100

0.803
  pilB Acinetobacter baylyi ADP1

51.237

100

0.519
  pilB Acinetobacter baumannii D1279779

51.439

99.463

0.512
  pilB Legionella pneumophila strain ERS1305867

48.393

100

0.485
  pilB Vibrio cholerae strain A1552

45.907

100

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

44.858

100

0.453
  pilB Vibrio campbellii strain DS40M4

43.262

100

0.436
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.382

92.665

0.365

Multiple sequence alignment