Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MON40_RS02435 Genome accession   NZ_CP094241
Coordinates   519703..521382 (-) Length   559 a.a.
NCBI ID   WP_003757129.1    Uniprot ID   A0A9X5P151
Organism   Neisseria macacae ATCC 33926     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 514703..526382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MON40_RS02405 (MON40_02405) - 514961..515812 (-) 852 WP_003757138.1 mechanosensitive ion channel family protein -
  MON40_RS02410 (MON40_02410) - 516116..516451 (+) 336 WP_003757137.1 hypothetical protein -
  MON40_RS02415 (MON40_02415) yacG 516654..516839 (-) 186 WP_003763805.1 DNA gyrase inhibitor YacG -
  MON40_RS02420 (MON40_02420) coaE 516894..517523 (-) 630 WP_003780213.1 dephospho-CoA kinase -
  MON40_RS02425 (MON40_02425) pilD 517525..518388 (-) 864 WP_003780212.1 A24 family peptidase Machinery gene
  MON40_RS02430 (MON40_02430) pilG 518390..519631 (-) 1242 WP_039863289.1 type II secretion system F family protein Machinery gene
  MON40_RS02435 (MON40_02435) pilF 519703..521382 (-) 1680 WP_003757129.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MON40_RS02440 (MON40_02440) bamE 521907..522380 (-) 474 WP_003757128.1 outer membrane protein assembly factor BamE -
  MON40_RS02445 (MON40_02445) - 522445..523704 (-) 1260 WP_003780206.1 YadA-like family protein -
  MON40_RS02450 (MON40_02450) apbC 524433..525512 (-) 1080 WP_003780202.1 iron-sulfur cluster carrier protein ApbC -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62225.71 Da        Isoelectric Point: 6.4397

>NTDB_id=668300 MON40_RS02435 WP_003757129.1 519703..521382(-) (pilF) [Neisseria macacae ATCC 33926]
MSIGLLRVLLQSQTITNAQAEHYGNALKSGKEILPMLFADGIISPRSLGELVARVFSYPLLDLHHYPRSNVITDVLSEEQ
MVQNRCIPIFRRGRKVYLAVSDPTQIQNFQKITFSSGVTIDLVVVPDDQLSSLLEWLGQRSTTILKEISEEQEAAQPSQA
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAATLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEDVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKREVERPPVPALKKAGFTDEDLAKDWKL
YRPVGCDSCRGKGFKGRVGVYEVMPITEEMQRVIMNNGTEVDIMNMAYKEGMVDLRRAGLLKVMQGLTSLEEVIAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=668300 MON40_RS02435 WP_003757129.1 519703..521382(-) (pilF) [Neisseria macacae ATCC 33926]
ATGAGTATCGGACTATTACGCGTACTGCTTCAAAGCCAGACCATTACAAATGCACAAGCTGAACATTACGGCAATGCCTT
GAAATCAGGGAAAGAAATCTTACCCATGCTTTTTGCGGACGGCATCATCTCTCCTAGAAGTTTAGGAGAATTAGTTGCAC
GAGTATTCAGCTACCCCTTATTGGATTTGCATCATTATCCTCGCAGCAATGTCATAACTGATGTTTTAAGCGAAGAACAG
ATGGTTCAAAATCGATGCATACCCATATTTCGTCGCGGCCGTAAAGTTTATCTGGCCGTATCAGACCCGACACAAATCCA
AAACTTCCAAAAAATCACTTTTTCTTCAGGTGTGACGATAGATTTAGTTGTCGTACCTGACGACCAGTTAAGCTCTCTTT
TAGAATGGTTAGGACAACGTTCTACCACCATCTTAAAAGAAATTAGTGAAGAACAAGAAGCTGCCCAGCCCTCTCAGGCG
CTTTATATTGACAATGAAGAGGCTGAAGATGGGCCTATTCCACGCTTTATTCACAAAACTTTATCAGATGCACTAAATGC
AGGCGCATCCGATATACATTTCGAATTTTATGAACAAATGGCTCGTGTCCGTTTTCGTGTAGATGGACAGCTAAGGGAAG
TGGTTCAACCCCCTGTTGCTGTACGCGGACAGCTGGCTTCACGTATCAAAGTAATGGCGCGTTTGGACATTTCGGAAAAA
CGCGTACCGCAAGATGGTCGTATTCAGATTGCTTTCCATAAACACGGTCGCCCCATTGATTTTCGTGTAAGTACGTTACC
TACGCTTTTTGGCGAAAAAGTGGTAATGCGTATTCTGAATTCTGATGCAGCAACTTTAAATATTGACCAACTGGGATTTG
AGCCTTTCCAAAAAGAAATGCTGCTTGAGGCTATTCATCGGCCTTATGGCATGGTATTGGTAACAGGTCCGACAGGCTCC
GGTAAAACCGTATCACTTTATACTTGCTTGAATATTTTAAATACAGAAGACGTCAATATTTCCACCGCAGAAGACCCGGC
CGAGATTAATTTGCCCGGCATCAACCAAGTCAACGTCAATGATAAACAAGGTTTGACTTTTGCTGCGGCGTTGAAATCTT
TCTTGCGACAAGACCCTGACATTATCATGGTAGGTGAGATTCGGGACTTGGAAACCGCGGATATTGCCATCAAAGCGGCA
CAAACCGGACATATGGTGTTTTCAACGTTGCATACCAATAACGCGCCTGCGACCCTATCCCGTATGTTGAACATGGGGGT
TGCCCCATTCAACATTGCCAGTTCCGTCAGCTTAATTATGGCGCAACGACTATTGCGTCGTCTGTGTTCGAGCTGTAAGC
GTGAGGTTGAACGTCCTCCAGTTCCCGCATTAAAAAAAGCAGGATTTACAGATGAGGATTTAGCAAAGGATTGGAAACTC
TACCGTCCTGTCGGTTGCGATAGTTGTCGTGGTAAAGGCTTTAAAGGCCGTGTCGGTGTCTACGAAGTAATGCCGATTAC
CGAGGAAATGCAACGTGTCATTATGAATAATGGTACGGAAGTTGATATTATGAACATGGCCTATAAAGAAGGCATGGTCG
ATTTACGCCGTGCAGGCTTATTAAAAGTTATGCAAGGGTTGACTTCATTAGAAGAAGTGATTGCTCACACTAACGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

80.143

100

0.801
  pilB Acinetobacter baylyi ADP1

51.146

100

0.519
  pilB Acinetobacter baumannii D1279779

51.439

99.463

0.512
  pilB Legionella pneumophila strain ERS1305867

49.434

94.812

0.469
  pilB Vibrio cholerae strain A1552

45.552

100

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

45.035

100

0.454
  pilB Vibrio campbellii strain DS40M4

43.44

100

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.722

95.17

0.369

Multiple sequence alignment