Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MOQ58_RS25210 Genome accession   NZ_CP093428
Coordinates   5531924..5533624 (+) Length   566 a.a.
NCBI ID   WP_223481777.1    Uniprot ID   -
Organism   Pseudomonas migulae strain 8R6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5526924..5538624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MOQ58_RS25190 (MOQ58_25150) - 5527091..5530027 (+) 2937 WP_207286524.1 DUF748 domain-containing protein -
  MOQ58_RS25195 (MOQ58_25155) - 5530027..5530323 (+) 297 WP_192419982.1 DUF2845 domain-containing protein -
  MOQ58_RS25200 (MOQ58_25160) - 5530599..5530952 (-) 354 WP_008042835.1 BON domain-containing protein -
  MOQ58_RS25205 (MOQ58_25165) - 5531282..5531698 (-) 417 WP_192419980.1 pilin -
  MOQ58_RS25210 (MOQ58_25170) pilB 5531924..5533624 (+) 1701 WP_223481777.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MOQ58_RS25215 (MOQ58_25175) pilC 5533627..5534844 (+) 1218 WP_280162238.1 type II secretion system F family protein Machinery gene
  MOQ58_RS25220 (MOQ58_25180) pilD 5534846..5535718 (+) 873 WP_008033208.1 A24 family peptidase Machinery gene
  MOQ58_RS25225 (MOQ58_25185) coaE 5535715..5536338 (+) 624 WP_223414094.1 dephospho-CoA kinase -
  MOQ58_RS25230 (MOQ58_25190) yacG 5536335..5536535 (+) 201 WP_008033210.1 DNA gyrase inhibitor YacG -
  MOQ58_RS25235 (MOQ58_25195) - 5536544..5536759 (-) 216 WP_008033212.1 hypothetical protein -
  MOQ58_RS25240 (MOQ58_25200) - 5536824..5537513 (-) 690 WP_192227990.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62548.61 Da        Isoelectric Point: 5.9727

>NTDB_id=664660 MOQ58_RS25210 WP_223481777.1 5531924..5533624(+) (pilB) [Pseudomonas migulae strain 8R6]
MNDIALSGLAKQLVLAELLTDQSAQQAYQQAQRHKISLVSYLVQNKLVKSRQVAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGVSDPTNHQAINDIQFSTGLNTEAILVEDDKLSDAIEKFFDSHASGLEDMADVDLDG
MDIESVDDHQQDAIAGQDADDAPVVRFVHKMLLDAIRSGSSDLHFEPYEKTYRVRMRTDGMLREVAKPPIQLANRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNAKQGLGFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSQCKKPIDIPREALIKEGFPE
ERIGAFTIYEPVGCDQCNGGYKGRVGIYEVVKNTAGLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=664660 MOQ58_RS25210 WP_223481777.1 5531924..5533624(+) (pilB) [Pseudomonas migulae strain 8R6]
ATGAATGACATCGCCCTTAGCGGTCTGGCCAAGCAATTGGTGCTTGCCGAGCTGCTCACCGACCAAAGCGCGCAACAGGC
GTATCAGCAAGCCCAACGCCACAAAATCTCCCTGGTCAGTTATCTGGTGCAGAACAAACTGGTGAAAAGTCGCCAGGTCG
CCGAGATAGCCTCGGAGCATTTCGGCATGGCGCTGCTCGACCTCAATTGCCTGGACAAGGAAACCCAACCCAAGGGTCTG
GTCAGCGAAAAACTGGTTCGCCAGCACCACGCCCTGCCTCTCTGGCGGCGTGGCAACAAGTTGTTCGTGGGGGTTTCCGA
CCCGACCAATCACCAGGCCATCAACGACATCCAGTTCAGCACCGGGCTGAACACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGATGCCATCGAGAAGTTCTTCGACAGCCATGCCAGCGGACTGGAAGACATGGCCGACGTCGACCTCGATGGC
ATGGACATCGAGTCGGTCGATGACCACCAACAGGACGCCATCGCCGGACAGGATGCCGACGACGCGCCGGTGGTGCGCTT
CGTTCACAAGATGCTGCTGGACGCGATCAGGAGTGGCTCTTCCGACCTGCATTTCGAGCCCTACGAAAAAACCTACCGCG
TGCGGATGCGCACCGACGGAATGCTGCGTGAAGTCGCCAAGCCGCCGATTCAACTGGCCAACCGCATCGCCGCCCGCCTG
AAAGTCATGGCCAGCCTCGACATCTCCGAGCGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAGAGCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGATCCGTCGAGCG
CACAAATGGGCATCGACGCCCTCGGCTACGAGCCCGACCAGAAAGACCTCTACATGGCCGCGCTGAAGCAGCCGCAGGGG
ATGATTCTGGTGACCGGCCCCACGGGGTCGGGCAAGACCGTATCGCTCTACACCGGCCTGAACATTCTCAACACCGTGGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAACGCCAAGCAAG
GACTGGGTTTTGCCCAGGCGTTGCGCTCCTTCTTGCGGCAGGACCCTGATGTGATCATGGTCGGAGAAATCCGCGACCTG
GAAACCGCCGAAATTGCCATCAAGGCCGCCCAGACCGGGCATCTGGTGCTGTCGACCCTGCACACCAACAGCGCCGCTGA
AACCCTGACCCGCCTGCACAACATGGGCATTCAGGGGTTCAACATCGCGACGTCCGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGCCAGTGCAAGAAGCCCATCGATATTCCCCGCGAGGCATTGATCAAGGAAGGCTTCCCCGAG
GAACGGATTGGCGCATTCACGATCTATGAGCCAGTCGGTTGCGATCAGTGCAACGGTGGCTACAAGGGGCGCGTGGGGAT
CTATGAAGTGGTGAAGAACACAGCAGGCCTGCAACGGCTGATCATGGCCGAAGGCAATTCGCTGGAAATCGATATCCAGA
TGCGCAAGGACGGCTTCAACGACCTGCGCACTTCCGGGCTGATCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.115

99.823

0.59
  pilB Acinetobacter baumannii D1279779

58.511

99.647

0.583
  pilB Legionella pneumophila strain ERS1305867

56.814

99.823

0.567
  pilB Vibrio cholerae strain A1552

50.973

99.823

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

50.802

99.117

0.504
  pilB Vibrio campbellii strain DS40M4

49.123

100

0.495
  pilF Neisseria gonorrhoeae MS11

49.556

99.47

0.493
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.319

93.463

0.367