Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   MN088_RS11490 Genome accession   NZ_CP093413
Coordinates   2242597..2243670 (-) Length   357 a.a.
NCBI ID   WP_010906319.1    Uniprot ID   A0A552YT77
Organism   Lactococcus lactis strain AH1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2202506..2250247 2242597..2243670 within 0


Gene organization within MGE regions


Location: 2202506..2250247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN088_RS11200 (MN088_11200) - 2202506..2203243 (-) 738 WP_010906311.1 metal ABC transporter ATP-binding protein -
  MN088_RS11205 (MN088_11205) - 2203420..2204262 (-) 843 WP_010906312.1 metal ABC transporter substrate-binding protein -
  MN088_RS11210 (MN088_11210) - 2204259..2204696 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  MN088_RS11215 (MN088_11215) comGG 2204777..2205061 (-) 285 WP_010906314.1 competence type IV pilus minor pilin ComGG Machinery gene
  MN088_RS11220 (MN088_11220) comGF 2205100..2205546 (-) 447 WP_031296844.1 competence type IV pilus minor pilin ComGF Machinery gene
  MN088_RS11225 (MN088_11225) comGE 2205509..2205805 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  MN088_RS11230 (MN088_11230) comGD 2205777..2206193 (-) 417 WP_021216554.1 competence type IV pilus minor pilin ComGD Machinery gene
  MN088_RS11235 (MN088_11235) comGC 2206168..2206437 (-) 270 WP_023349160.1 competence type IV pilus major pilin ComGC Machinery gene
  MN088_RS11240 (MN088_11240) - 2206584..2207108 (-) 525 WP_014570795.1 GNAT family N-acetyltransferase -
  MN088_RS11245 (MN088_11245) - 2207188..2207967 (-) 780 WP_242166666.1 peptidoglycan amidohydrolase family protein -
  MN088_RS11250 (MN088_11250) - 2207967..2208254 (-) 288 WP_242166667.1 phage holin -
  MN088_RS11255 (MN088_11255) - 2208268..2208492 (-) 225 WP_010905920.1 hemolysin XhlA family protein -
  MN088_RS11260 (MN088_11260) - 2208505..2208726 (-) 222 WP_242166668.1 hypothetical protein -
  MN088_RS11265 (MN088_11265) - 2208746..2213902 (-) 5157 WP_242166669.1 interleukin-like EMT inducer domain-containing protein -
  MN088_RS11270 (MN088_11270) - 2213902..2215458 (-) 1557 WP_242166670.1 distal tail protein Dit -
  MN088_RS11275 (MN088_11275) - 2215459..2218071 (-) 2613 WP_242166671.1 phage tail tape measure protein -
  MN088_RS11280 (MN088_11280) - 2218061..2218768 (-) 708 WP_031561043.1 Gp15 family bacteriophage protein -
  MN088_RS11285 (MN088_11285) - 2218784..2219191 (-) 408 WP_003131323.1 hypothetical protein -
  MN088_RS11290 (MN088_11290) - 2219248..2219724 (-) 477 WP_014570559.1 hypothetical protein -
  MN088_RS11295 (MN088_11295) - 2219735..2220169 (-) 435 WP_003131321.1 minor capsid protein -
  MN088_RS11300 (MN088_11300) - 2220169..2220498 (-) 330 WP_003131320.1 hypothetical protein -
  MN088_RS11305 (MN088_11305) - 2220495..2220839 (-) 345 WP_242166672.1 putative minor capsid protein -
  MN088_RS11310 (MN088_11310) - 2220829..2221230 (-) 402 WP_014570556.1 hypothetical protein -
  MN088_RS11315 (MN088_11315) - 2221304..2221540 (-) 237 WP_014570555.1 Ig-like domain-containing protein -
  MN088_RS11320 (MN088_11320) - 2221569..2222486 (-) 918 WP_242166673.1 phage capsid protein -
  MN088_RS11325 (MN088_11325) - 2222501..2223562 (-) 1062 WP_242166674.1 XkdF-like putative serine protease domain-containing protein -
  MN088_RS11330 (MN088_11330) - 2223578..2224408 (-) 831 WP_242166675.1 phage minor head protein -
  MN088_RS11335 (MN088_11335) - 2224401..2225930 (-) 1530 WP_058221397.1 phage portal protein -
  MN088_RS11340 (MN088_11340) terL 2225943..2227394 (-) 1452 WP_242166676.1 phage terminase large subunit -
  MN088_RS11345 (MN088_11345) - 2227375..2227872 (-) 498 WP_014570801.1 hypothetical protein -
  MN088_RS11350 (MN088_11350) - 2227901..2229058 (-) 1158 WP_242166677.1 DNA modification methylase -
  MN088_RS11360 (MN088_11360) - 2229535..2229957 (-) 423 WP_242166678.1 RinA family protein -
  MN088_RS11365 (MN088_11365) - 2230035..2230343 (-) 309 WP_242166679.1 hypothetical protein -
  MN088_RS11370 (MN088_11370) - 2230504..2230647 (+) 144 WP_242166756.1 hypothetical protein -
  MN088_RS11375 (MN088_11375) - 2230577..2230813 (-) 237 WP_015967998.1 DUF1660 domain-containing protein -
  MN088_RS11380 (MN088_11380) - 2230810..2231028 (-) 219 WP_011834792.1 hypothetical protein -
  MN088_RS11385 (MN088_11385) - 2231047..2231385 (-) 339 WP_242166680.1 DUF1140 family protein -
  MN088_RS11390 (MN088_11390) dut 2231386..2231805 (-) 420 WP_242166681.1 dUTP diphosphatase -
  MN088_RS11395 (MN088_11395) - 2231802..2232479 (-) 678 WP_242166682.1 DUF1642 domain-containing protein -
  MN088_RS11400 (MN088_11400) - 2232451..2232810 (-) 360 WP_150890987.1 hypothetical protein -
  MN088_RS11405 (MN088_11405) - 2233002..2233208 (-) 207 WP_242166683.1 hypothetical protein -
  MN088_RS11410 (MN088_11410) - 2233319..2233558 (-) 240 WP_058225578.1 DUF1031 family protein -
  MN088_RS11415 (MN088_11415) - 2233555..2233860 (-) 306 WP_242166684.1 hypothetical protein -
  MN088_RS11420 (MN088_11420) - 2233865..2234253 (-) 389 Protein_2229 RusA family crossover junction endodeoxyribonuclease -
  MN088_RS11425 (MN088_11425) - 2234266..2234508 (-) 243 WP_242166685.1 L-rhamnose isomerase -
  MN088_RS11430 (MN088_11430) - 2234501..2235415 (-) 915 WP_242166686.1 phage replisome organizer N-terminal domain-containing protein -
  MN088_RS11435 (MN088_11435) - 2235679..2236605 (-) 927 WP_242166687.1 RecT family recombinase -
  MN088_RS11440 (MN088_11440) - 2236602..2237435 (-) 834 WP_240819231.1 hypothetical protein -
  MN088_RS11445 (MN088_11445) - 2237539..2237781 (-) 243 WP_023164375.1 hypothetical protein -
  MN088_RS13565 - 2237794..2237916 (-) 123 WP_023164646.1 hypothetical protein -
  MN088_RS11450 (MN088_11450) - 2237913..2238095 (-) 183 WP_003130605.1 hypothetical protein -
  MN088_RS11455 (MN088_11455) - 2238111..2238800 (-) 690 WP_014570818.1 phage regulatory protein -
  MN088_RS11460 (MN088_11460) - 2238859..2239092 (-) 234 WP_014570819.1 transcriptional regulator -
  MN088_RS11465 (MN088_11465) - 2239299..2239679 (+) 381 WP_031561101.1 XRE family transcriptional regulator -
  MN088_RS11470 (MN088_11470) - 2239690..2240274 (+) 585 WP_058221714.1 hypothetical protein -
  MN088_RS11475 (MN088_11475) - 2240330..2240869 (+) 540 WP_023189015.1 PH domain-containing protein -
  MN088_RS11480 (MN088_11480) - 2240989..2242446 (+) 1458 WP_031561103.1 recombinase family protein -
  MN088_RS11485 (MN088_11485) - 2242443..2242583 (-) 141 WP_023164653.1 hypothetical protein -
  MN088_RS11490 (MN088_11490) comGB 2242597..2243670 (-) 1074 WP_010906319.1 competence type IV pilus assembly protein ComGB Machinery gene
  MN088_RS11495 (MN088_11495) comGA 2243564..2244502 (-) 939 WP_010906320.1 competence type IV pilus ATPase ComGA Machinery gene
  MN088_RS11500 (MN088_11500) - 2244622..2249538 (-) 4917 WP_031296849.1 PolC-type DNA polymerase III -
  MN088_RS11505 (MN088_11505) - 2249711..2250247 (-) 537 WP_010906322.1 hypothetical protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40330.51 Da        Isoelectric Point: 10.3000

>NTDB_id=664541 MN088_RS11490 WP_010906319.1 2242597..2243670(-) (comGB) [Lactococcus lactis strain AH1]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEQFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=664541 MN088_RS11490 WP_010906319.1 2242597..2243670(-) (comGB) [Lactococcus lactis strain AH1]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCGTTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACAATTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCGGGAATATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A552YT77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

83.578

95.518

0.798
  comYB Streptococcus gordonii str. Challis substr. CH1

53.959

95.518

0.515
  comYB Streptococcus mutans UA140

53.372

95.518

0.51
  comYB Streptococcus mutans UA159

53.079

95.518

0.507
  comGB/cglB Streptococcus mitis SK321

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae TIGR4

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae Rx1

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae D39

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae R6

51.045

93.838

0.479
  comGB/cglB Streptococcus mitis NCTC 12261

50.448

93.838

0.473