Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PSCI_RS20235 Genome accession   NZ_AP014637
Coordinates   4495289..4496467 (+) Length   392 a.a.
NCBI ID   WP_045490511.1    Uniprot ID   A0A077LKP6
Organism   Pseudomonas sp. StFLB209     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4490289..4501467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSCI_RS20195 (PSCI_4005) - 4490447..4490746 (-) 300 WP_045494637.1 type II toxin-antitoxin system RelE/ParE family toxin -
  PSCI_RS20200 - 4491180..4491587 (+) 408 WP_052483442.1 hypothetical protein -
  PSCI_RS29185 (PSCI_4006) - 4491596..4491856 (+) 261 WP_144403291.1 hypothetical protein -
  PSCI_RS29845 - 4491853..4492064 (-) 212 Protein_4014 IS66 family transposase -
  PSCI_RS29550 (PSCI_4007) - 4492273..4492422 (+) 150 WP_173426693.1 hypothetical protein -
  PSCI_RS20205 (PSCI_4008) - 4492455..4492712 (-) 258 WP_052483445.1 hypothetical protein -
  PSCI_RS20210 (PSCI_4009) ykgO 4493014..4493163 (+) 150 WP_045490502.1 type B 50S ribosomal protein L36 -
  PSCI_RS30000 - 4493215..4493349 (-) 135 WP_257105868.1 hypothetical protein -
  PSCI_RS20215 (PSCI_4010) - 4493525..4493842 (-) 318 WP_045490504.1 SMR family transporter -
  PSCI_RS20220 (PSCI_4011) - 4494067..4494510 (+) 444 WP_045490506.1 hemerythrin domain-containing protein -
  PSCI_RS20225 (PSCI_4012) - 4494561..4494908 (+) 348 WP_045490508.1 hypothetical protein -
  PSCI_RS20230 (PSCI_4013) - 4494917..4495192 (-) 276 WP_045490510.1 peptidylprolyl isomerase -
  PSCI_RS20235 (PSCI_4014) pilU 4495289..4496467 (+) 1179 WP_045490511.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSCI_RS20240 (PSCI_4015) - 4496485..4496973 (-) 489 WP_045490512.1 DMT family transporter -
  PSCI_RS20245 (PSCI_4016) - 4497072..4497977 (+) 906 WP_045490513.1 LysR family transcriptional regulator -
  PSCI_RS20250 (PSCI_4017) - 4497991..4499568 (-) 1578 WP_045490514.1 ABC transporter ATP-binding protein -
  PSCI_RS20255 (PSCI_4018) - 4499570..4500592 (-) 1023 WP_045490516.1 ABC transporter permease -

Sequence

Protein


Download         Length: 392 a.a.        Molecular weight: 42969.01 Da        Isoelectric Point: 6.3762

>NTDB_id=66453 PSCI_RS20235 WP_045490511.1 4495289..4496467(+) (pilU) [Pseudomonas sp. StFLB209]
MDFQALLKTLATQDGSDLYLSTGAPPCAKFNGVLRPLGTETLKPGEVAKVADAIMDDEQKTAFERELEMNLAVSLAGVGR
FRINIFKQRNEVSIVARNIKLDIPKFEDLHLPPVLLDVVMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIINQREVGVDTRSFHNALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGSPTIRDLIQRNELTELKSIMEKSGNLGMQTFDSAL
FNLVVEGAISEEEAIKHADSKNNVRLRLKLHGEGGVTAAPTAAPAAPVGVASPSMTQWGLVEDDDEPGTTQS

Nucleotide


Download         Length: 1179 bp        

>NTDB_id=66453 PSCI_RS20235 WP_045490511.1 4495289..4496467(+) (pilU) [Pseudomonas sp. StFLB209]
ATGGATTTTCAGGCATTACTCAAGACCCTGGCCACTCAGGACGGTTCGGACCTTTATCTGTCTACTGGCGCGCCACCCTG
CGCCAAGTTCAACGGTGTGTTGCGGCCATTGGGTACTGAAACGCTCAAGCCCGGTGAAGTGGCCAAGGTCGCTGATGCGA
TCATGGATGACGAGCAGAAAACCGCCTTCGAGCGTGAGTTGGAAATGAACCTGGCCGTGTCCCTGGCCGGGGTCGGGCGG
TTTCGGATCAATATCTTCAAGCAGCGCAACGAAGTGTCGATCGTGGCGCGCAATATCAAGCTGGATATTCCGAAATTCGA
AGACCTGCACCTGCCGCCAGTGCTGCTTGATGTGGTGATGGAAAAGCATGGGCTGGTGCTATTTGTCGGTGCCACCGGTT
CAGGCAAATCGACGTCTTTGGCGGCACTGATCGATTATCGCAACCGCAATGCCAGCGGGCATATCATCACCATCGAAGAC
CCGGTGGAATTCATTCACCGGCACAAGAAGTCGATCATCAACCAGCGTGAGGTCGGGGTCGATACCCGCAGTTTTCATAA
CGCGCTGAAGAACACCCTGCGCCAGGCACCGGATGTGATCCTGATCGGCGAGATCCGCGACCGGGAAACCATGGAGCATG
CGCTGGCGTTTGCTGACACCGGGCATCTGGCCATTTCGACCTTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTTTTCCCTGAAGAGCGCCGTGCGCAACTGCTGCATGACTTGGGCAATAACCTCAAAGCGTTCGTTTCCCAGCG
TCTGGTGCGCACCCCGGACGGCAAGCGGCGCGCGGCGGTCGAGGTGATGATGGGCTCACCGACCATCCGCGACTTGATTC
AGCGTAACGAGTTGACCGAGCTGAAAAGCATCATGGAGAAGTCCGGCAACCTGGGCATGCAGACCTTCGATTCGGCATTG
TTCAATCTGGTGGTGGAGGGTGCGATCAGCGAAGAAGAAGCCATCAAGCATGCTGACTCGAAGAACAATGTGCGCTTGCG
TCTCAAGTTGCATGGTGAGGGCGGCGTGACTGCCGCGCCGACCGCAGCACCAGCGGCGCCGGTGGGGGTCGCCAGCCCGA
GCATGACTCAGTGGGGCCTGGTGGAGGATGACGACGAACCCGGCACGACACAGTCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A077LKP6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.497

90.306

0.51
  pilU Acinetobacter baylyi ADP1

52.101

91.071

0.474
  pilU Vibrio cholerae strain A1552

52.571

89.286

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.052

97.449

0.39
  pilT Pseudomonas aeruginosa PAK

42.274

87.5

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.691

87.5

0.365

Multiple sequence alignment