Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MMZ75_RS14010 Genome accession   NZ_CP093016
Coordinates   3003682..3005382 (-) Length   566 a.a.
NCBI ID   WP_241474364.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H15     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2998682..3010382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMZ75_RS13970 (MMZ75_13965) - 2998853..2999278 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  MMZ75_RS13975 (MMZ75_13970) - 2999275..2999745 (+) 471 WP_003103866.1 hypothetical protein -
  MMZ75_RS13980 (MMZ75_13975) - 2999742..3000431 (+) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  MMZ75_RS13985 (MMZ75_13980) - 3000537..3000746 (+) 210 WP_003094660.1 hypothetical protein -
  MMZ75_RS13990 (MMZ75_13985) yacG 3000783..3000983 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  MMZ75_RS13995 (MMZ75_13990) coaE 3000980..3001591 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  MMZ75_RS14000 (MMZ75_13995) pilD 3001588..3002460 (-) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  MMZ75_RS14005 (MMZ75_14000) pilC 3002461..3003678 (-) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  MMZ75_RS14010 (MMZ75_14005) pilB 3003682..3005382 (-) 1701 WP_241474364.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MMZ75_RS14015 (MMZ75_14010) - 3005613..3006077 (+) 465 WP_003122079.1 pilin -
  MMZ75_RS14020 (MMZ75_14015) - 3006153..3007538 (+) 1386 WP_003122080.1 O-antigen ligase -
  MMZ75_RS14030 (MMZ75_14025) nadC 3007719..3008567 (-) 849 WP_003122081.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62255.20 Da        Isoelectric Point: 5.5785

>NTDB_id=661438 MMZ75_RS14010 WP_241474364.1 3003682..3005382(-) (pilB) [Pseudomonas aeruginosa strain H15]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQAPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=661438 MMZ75_RS14010 WP_241474364.1 3003682..3005382(-) (pilB) [Pseudomonas aeruginosa strain H15]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGTGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCACCAGGCGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCTATGGACGCTCTCGGCGACCTCGGTGACATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGACCAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCACTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGTCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCTGAAGACCCTGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGTCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGCCCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCCGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.368

100

0.578
  pilB Acinetobacter baylyi ADP1

56.614

100

0.567
  pilB Legionella pneumophila strain ERS1305867

54.321

100

0.544
  pilB Vibrio cholerae strain A1552

51.342

98.763

0.507
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

51.97

94.17

0.489
  pilB Vibrio campbellii strain DS40M4

51.032

94.17

0.481
  pilB/pilB1 Synechocystis sp. PCC 6803

37.601

100

0.41
  pilF Thermus thermophilus HB27

38.489

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.545

97.173

0.375