Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MM141_RS23815 Genome accession   NZ_CP093012
Coordinates   5111814..5113514 (+) Length   566 a.a.
NCBI ID   WP_009875880.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H20     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5113518..5122222 5111814..5113514 flank 4


Gene organization within MGE regions


Location: 5111814..5122222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MM141_RS23815 (MM141_23825) pilB 5111814..5113514 (+) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MM141_RS23820 (MM141_23830) pilC 5113518..5114738 (+) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  MM141_RS23825 (MM141_23835) pilD 5114742..5115614 (+) 873 WP_009875878.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  MM141_RS23830 (MM141_23840) coaE 5115611..5116222 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  MM141_RS23835 (MM141_23845) yacG 5116219..5116419 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  MM141_RS23840 (MM141_23850) - 5116456..5116665 (-) 210 WP_003094660.1 hypothetical protein -
  MM141_RS23845 (MM141_23855) - 5116771..5117460 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  MM141_RS23850 (MM141_23860) - 5117457..5117927 (-) 471 WP_003094664.1 hypothetical protein -
  MM141_RS23855 (MM141_23865) - 5117924..5118349 (-) 426 WP_009875877.1 GNAT family N-acetyltransferase -
  MM141_RS23860 (MM141_23870) - 5118482..5119111 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  MM141_RS23865 (MM141_23875) - 5119108..5119557 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  MM141_RS23870 (MM141_23880) - 5119583..5119753 (+) 171 WP_003094672.1 DUF3094 family protein -
  MM141_RS23875 (MM141_23885) - 5119817..5121124 (+) 1308 WP_003112837.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62383.27 Da        Isoelectric Point: 5.5590

>NTDB_id=661334 MM141_RS23815 WP_009875880.1 5111814..5113514(+) (pilB) [Pseudomonas aeruginosa strain H20]
MNDSIQLSGLSRQLVQANLLDEKTALQAQTQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDRESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDAANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFENATDGLAGLDDVDLE
GLDVGVKETSGQEDTGAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=661334 MM141_RS23815 WP_009875880.1 5111814..5113514(+) (pilB) [Pseudomonas aeruginosa strain H20]
ATGAACGACAGCATCCAACTGAGCGGTCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCCTGCA
GGCCCAGACGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGACAGGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAGCACCGGGTCATTCCCCTGTGGCGACGGGGCAACAAGTTGTTCGTAGGCATCTC
CGACGCCGCCAACCACCAGGCCATCAACGACGTCCAGTTCAGCACTGGCCTGACGACCGAGGCCATTCTGGTCGAGGACG
ACAAGCTCGGCCTTGCCATCGACAAGCTATTCGAGAATGCAACCGACGGCTTGGCAGGTCTCGATGACGTGGACCTGGAA
GGACTGGATGTAGGCGTCAAGGAAACGTCCGGACAAGAAGATACTGGCGCGGAGGCAGACGACGCTCCCGTCGTCCGCTT
CGTCAACAAGATGCTGCTGGACGCTATCAAAGGCGGGTCTTCCGACTTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTTCACGAAGTGGCCAAGCCGCCGATTCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGATGCCCTGGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTGTACACCGGTCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTACCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAGCACGACGTACCGAAGGAAACCCTGCTTCACGAAGGTTTCCCAGAA
GAGTTGATCGGCACCTTCAAGCTGTATTCACCAGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.719

100

0.581
  pilB Acinetobacter baylyi ADP1

57.371

99.47

0.571
  pilB Legionella pneumophila strain ERS1305867

54.77

100

0.548
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

50.444

99.47

0.502
  pilB Vibrio cholerae strain A1552

50

100

0.5
  pilB Vibrio campbellii strain DS40M4

49.113

99.647

0.489
  pilF Thermus thermophilus HB27

39.855

97.527

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.417

93.11

0.376