Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MM141_RS23825 Genome accession   NZ_CP093012
Coordinates   5114742..5115614 (+) Length   290 a.a.
NCBI ID   WP_009875878.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5113518..5122222 5114742..5115614 within 0


Gene organization within MGE regions


Location: 5113518..5122222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MM141_RS23820 (MM141_23830) pilC 5113518..5114738 (+) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  MM141_RS23825 (MM141_23835) pilD 5114742..5115614 (+) 873 WP_009875878.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  MM141_RS23830 (MM141_23840) coaE 5115611..5116222 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  MM141_RS23835 (MM141_23845) yacG 5116219..5116419 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  MM141_RS23840 (MM141_23850) - 5116456..5116665 (-) 210 WP_003094660.1 hypothetical protein -
  MM141_RS23845 (MM141_23855) - 5116771..5117460 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  MM141_RS23850 (MM141_23860) - 5117457..5117927 (-) 471 WP_003094664.1 hypothetical protein -
  MM141_RS23855 (MM141_23865) - 5117924..5118349 (-) 426 WP_009875877.1 GNAT family N-acetyltransferase -
  MM141_RS23860 (MM141_23870) - 5118482..5119111 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  MM141_RS23865 (MM141_23875) - 5119108..5119557 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  MM141_RS23870 (MM141_23880) - 5119583..5119753 (+) 171 WP_003094672.1 DUF3094 family protein -
  MM141_RS23875 (MM141_23885) - 5119817..5121124 (+) 1308 WP_003112837.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31811.84 Da        Isoelectric Point: 7.7068

>NTDB_id=661336 MM141_RS23825 WP_009875878.1 5114742..5115614(+) (pilD) [Pseudomonas aeruginosa strain H20]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLASYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=661336 MM141_RS23825 WP_009875878.1 5114742..5115614(+) (pilD) [Pseudomonas aeruginosa strain H20]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGCCAGCTACCTGGCGCTGGGCGGCAAGTGTTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATACTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.714

96.552

0.538
  pilD Vibrio campbellii strain DS40M4

53.004

97.586

0.517
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

48.921

95.862

0.469