Detailed information NTG0066106

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: M3B_RS05435
• Genome accession: NZ_AP014596
• Coordinates: 1001641..1002477 (+)
• Length: 278 a.a.
• NCBI ID: WP_011054501.1
• Uniprot ID: A0A5S4TKN4
• Organism: Streptococcus pyogenes strain M3-b
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 996641..1007477

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M3B_RS05405 (M3B_1108)	-	997189..997563 (+)	375	WP_011054498.1	hypothetical protein	-
M3B_RS05410 (M3B_1109)	-	997613..998524 (-)	912	WP_002984631.1	diacylglycerol/lipid kinase family protein	-
M3B_RS05415 (M3B_1110)	trhA	998641..999291 (-)	651	WP_002984629.1	PAQR family membrane homeostasis protein TrhA	-
M3B_RS05420 (M3B_1111)	-	999288..999728 (-)	441	WP_002989774.1	DUF1836 domain-containing protein	-
M3B_RS05425 (M3B_1112)	ylqF	999947..1000795 (+)	849	WP_011054499.1	ribosome biogenesis GTPase YlqF	-
M3B_RS05430 (M3B_1113)	-	1000785..1001576 (+)	792	WP_011054500.1	ribonuclease HII	-
M3B_RS05435 (M3B_1114)	dprA	1001641..1002477 (+)	837	WP_011054501.1	DNA-processing protein DprA	Machinery gene
M3B_RS05440 (M3B_1115)	topA	1002583..1004712 (+)	2130	WP_011054502.1	type I DNA topoisomerase	-
M3B_RS05445 (M3B_1116)	trmFO	1004847..1006193 (+)	1347	WP_032461335.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-

Sequence

Protein

• Length: 278 a.a.
• Molecular weight: 31087.14 Da
• Isoelectric Point: 9.4248

>NTDB_id=66106 M3B_RS05435 WP_011054501.1 1001641..1002477(+) (dprA) [Streptococcus pyogenes strain M3-b]
MNHFELYKLKKAGLTNKNILNILDYQKHQEKSLSLRDMAVVSGCKHPSHFIEAYKQLDIQKLKMEFKQFPSISILDKHYP
MALKEIYNPPVLLFFQGNLDLLEKPKLAIVGSRRSSDTGVKSVRKILKELGNRFVIVSGLARGIDTSAHLACLKNGGQTI
AIIGTGLDRFYPKENRELQTFLGKNHLVLTEYGPREEALSYHFPERNRIIAGLSRGILVVEAKNRSGSLITCQIGIEEGR
DIFAVPGNILDGKSEGCLQLIKEGATCVTSGMDILSEY

Nucleotide

• Length: 837 bp

>NTDB_id=66106 M3B_RS05435 WP_011054501.1 1001641..1002477(+) (dprA) [Streptococcus pyogenes strain M3-b]
GTGAATCATTTTGAACTTTATAAGCTAAAGAAAGCTGGACTGACTAATAAAAACATTCTCAATATTCTTGACTACCAAAA
ACACCAGGAAAAATCGTTGTCACTTCGAGATATGGCCGTTGTTTCTGGTTGTAAGCATCCGTCTCACTTTATAGAAGCCT
ATAAGCAGTTAGATATTCAAAAATTAAAAATGGAATTTAAACAATTTCCTAGTATTTCTATCTTAGATAAGCATTACCCA
ATGGCATTGAAAGAAATATACAATCCACCTGTCCTCTTGTTTTTTCAGGGAAATTTAGACCTTCTTGAGAAACCTAAATT
AGCCATTGTCGGCTCCAGACGCTCAAGCGATACCGGAGTAAAGTCTGTCCGTAAAATTCTTAAAGAACTCGGGAACCGTT
TTGTGATTGTTAGCGGACTTGCTCGAGGTATCGACACTAGTGCTCATTTAGCCTGCCTTAAAAATGGGGGACAAACCATC
GCTATTATTGGAACAGGGTTGGATCGCTTTTACCCTAAAGAAAATAGGGAGTTGCAAACTTTCTTAGGGAAAAATCATCT
TGTGCTAACAGAATACGGTCCAAGAGAAGAAGCTTTATCTTATCACTTTCCAGAACGGAATCGTATTATCGCAGGCCTTA
GCCGAGGTATTCTTGTCGTTGAAGCAAAAAATCGTTCAGGTTCCTTGATTACTTGTCAAATTGGGATAGAAGAAGGCCGA
GACATTTTTGCTGTCCCAGGAAACATTTTGGACGGGAAATCCGAAGGTTGCCTTCAGTTAATTAAAGAGGGAGCAACATG
CGTCACATCGGGCATGGATATCCTTTCAGAGTACTAA

Domains

Predicted by InterproScan.

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-62)

• DNA recombination-mediator protein A (DNA_processg_A) (68-269)

3D structure

• AlphaFold DB: A0A5S4TKN4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	67.266	100	0.673
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	60.072	100	0.601
dprA/cilB/dalA	Streptococcus pneumoniae D39	60.072	100	0.601
dprA/cilB/dalA	Streptococcus pneumoniae R6	60.072	100	0.601
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	60.072	100	0.601
dprA/cilB/dalA	Streptococcus mitis SK321	60.072	100	0.601
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	59.712	100	0.597
dprA	Lactococcus lactis subsp. cremoris KW2	57.194	100	0.572
dprA	Legionella pneumophila strain ERS1305867	42.857	85.612	0.367