Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   M3B_RS00595 Genome accession   NZ_AP014596
Coordinates   97110..98144 (+) Length   344 a.a.
NCBI ID   WP_011054115.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain M3-b     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 92110..103144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3B_RS00585 (M3B_0117) - 95778..96143 (+) 366 WP_002986560.1 DUF1033 family protein -
  M3B_RS00590 (M3B_0118) comYA 96236..97174 (+) 939 WP_011054114.1 competence type IV pilus ATPase ComGA Machinery gene
  M3B_RS00595 (M3B_0119) comYB 97110..98144 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  M3B_RS00600 (M3B_0120) comYC 98146..98472 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  M3B_RS00605 (M3B_0121) comGD 98447..98875 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  M3B_RS00610 comGE 98832..99116 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  M3B_RS00615 (M3B_0123) comYF 99109..99543 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  M3B_RS00620 (M3B_0124) comGG 99527..99853 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  M3B_RS00625 (M3B_0125) comYH 99951..100904 (+) 954 WP_011054117.1 class I SAM-dependent methyltransferase Machinery gene
  M3B_RS00630 (M3B_0126) - 100963..102159 (+) 1197 WP_010921803.1 acetate kinase -
  M3B_RS00635 (M3B_0127) - 102345..102653 (+) 309 WP_011054118.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39676.15 Da        Isoelectric Point: 9.7085

>NTDB_id=66076 M3B_RS00595 WP_011054115.1 97110..98144(+) (comYB) [Streptococcus pyogenes strain M3-b]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=66076 M3B_RS00595 WP_011054115.1 97110..98144(+) (comYB) [Streptococcus pyogenes strain M3-b]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGTCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.982

98.547

0.532
  comYB Streptococcus mutans UA159

53.982

98.547

0.532
  comYB Streptococcus gordonii str. Challis substr. CH1

50.588

98.837

0.5
  comGB/cglB Streptococcus mitis NCTC 12261

47.619

97.674

0.465
  comGB/cglB Streptococcus mitis SK321

47.748

96.802

0.462
  comGB/cglB Streptococcus pneumoniae Rx1

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae D39

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae R6

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae TIGR4

45.646

96.802

0.442
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404

Multiple sequence alignment