Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   M3B_RS00615 Genome accession   NZ_AP014596
Coordinates   99109..99543 (+) Length   144 a.a.
NCBI ID   WP_002992738.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain M3-b     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 94109..104543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3B_RS00585 (M3B_0117) - 95778..96143 (+) 366 WP_002986560.1 DUF1033 family protein -
  M3B_RS00590 (M3B_0118) comYA 96236..97174 (+) 939 WP_011054114.1 competence type IV pilus ATPase ComGA Machinery gene
  M3B_RS00595 (M3B_0119) comYB 97110..98144 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  M3B_RS00600 (M3B_0120) comYC 98146..98472 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  M3B_RS00605 (M3B_0121) comGD 98447..98875 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  M3B_RS00610 comGE 98832..99116 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  M3B_RS00615 (M3B_0123) comYF 99109..99543 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  M3B_RS00620 (M3B_0124) comGG 99527..99853 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  M3B_RS00625 (M3B_0125) comYH 99951..100904 (+) 954 WP_011054117.1 class I SAM-dependent methyltransferase Machinery gene
  M3B_RS00630 (M3B_0126) - 100963..102159 (+) 1197 WP_010921803.1 acetate kinase -
  M3B_RS00635 (M3B_0127) - 102345..102653 (+) 309 WP_011054118.1 hypothetical protein -
  M3B_RS00640 (M3B_0128) proC 102736..103506 (-) 771 WP_002992745.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16645.27 Da        Isoelectric Point: 10.2526

>NTDB_id=66078 M3B_RS00615 WP_002992738.1 99109..99543(+) (comYF) [Streptococcus pyogenes strain M3-b]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=66078 M3B_RS00615 WP_002992738.1 99109..99543(+) (comYF) [Streptococcus pyogenes strain M3-b]
TTGAGTAAACAATTAAGTAATATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGCTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment