Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MK801_RS02665 Genome accession   NZ_CP092748
Coordinates   517619..518266 (+) Length   215 a.a.
NCBI ID   WP_254255661.1    Uniprot ID   -
Organism   Lactococcus lactis strain 17M1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 512619..523266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK801_RS02640 (MK801_02640) - 512748..513926 (+) 1179 WP_015426943.1 SLC13 family permease -
  MK801_RS02645 (MK801_02645) - 514099..514662 (+) 564 WP_254255659.1 GNAT family N-acetyltransferase -
  MK801_RS02650 (MK801_02650) - 514766..515164 (+) 399 WP_057720189.1 hypothetical protein -
  MK801_RS02655 (MK801_02655) - 515269..516201 (+) 933 WP_015426941.1 ABC transporter ATP-binding protein -
  MK801_RS02660 (MK801_02660) - 516198..517559 (+) 1362 WP_254255660.1 ABC transporter permease -
  MK801_RS02665 (MK801_02665) comEA 517619..518266 (+) 648 WP_254255661.1 helix-hairpin-helix domain-containing protein Machinery gene
  MK801_RS02670 (MK801_02670) comEC 518247..520457 (+) 2211 WP_139008515.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MK801_RS02675 (MK801_02675) - 520742..521518 (+) 777 WP_015426937.1 alpha/beta hydrolase family protein -
  MK801_RS02680 (MK801_02680) - 521703..521918 (+) 216 WP_004255250.1 F0F1 ATP synthase subunit C -
  MK801_RS02685 (MK801_02685) atpB 521963..522676 (+) 714 WP_004255255.1 F0F1 ATP synthase subunit A -
  MK801_RS02690 (MK801_02690) atpF 522691..523197 (+) 507 WP_254255662.1 F0F1 ATP synthase subunit B -

Sequence

Protein


Download         Length: 215 a.a.        Molecular weight: 23652.77 Da        Isoelectric Point: 4.7655

>NTDB_id=659962 MK801_RS02665 WP_254255661.1 517619..518266(+) (comEA) [Lactococcus lactis strain 17M1]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVAKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSDFGDKTLEKLKDELCFE

Nucleotide


Download         Length: 648 bp        

>NTDB_id=659962 MK801_RS02665 WP_254255661.1 517619..518266(+) (comEA) [Lactococcus lactis strain 17M1]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTGGCAAAACCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCGCTTGAAGACTTGGGAAAAGTTTCTGATTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(70-125)

Helix-hairpin-helix motif (HHH_3)

(151-212)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

76.147

100

0.772
  comEA/celA/cilE Streptococcus mitis SK321

44.444

100

0.447
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

43.119

100

0.437
  comEA/celA/cilE Streptococcus pneumoniae Rx1

41.096

100

0.419
  comEA/celA/cilE Streptococcus pneumoniae D39

41.096

100

0.419
  comEA/celA/cilE Streptococcus pneumoniae R6

41.096

100

0.419
  comEA/celA/cilE Streptococcus mitis NCTC 12261

41.284

100

0.419
  comEA Latilactobacillus sakei subsp. sakei 23K

36.123

100

0.381