Detailed information NTG0659853

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MKW31_RS12315
• Genome accession: NZ_CP092715
• Coordinates: 2579319..2579933 (-)
• Length: 204 a.a.
• NCBI ID: WP_032866693.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 37-1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2574319..2584933

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MKW31_RS12285 (MKW31_12265)	rpsT	2574622..2574888 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
MKW31_RS12290 (MKW31_12270)	holA	2574905..2575946 (-)	1042	Protein_2373	DNA polymerase III subunit delta	-
MKW31_RS12295 (MKW31_12275)	-	2575986..2576137 (-)	152	Protein_2374	hypothetical protein	-
MKW31_RS12300 (MKW31_12280)	-	2576178..2576312 (+)	135	WP_003152870.1	YqzM family protein	-
MKW31_RS12305 (MKW31_12285)	comEC	2576331..2578682 (-)	2352	WP_251257463.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MKW31_RS12310 (MKW31_12290)	-	2578683..2579252 (-)	570	WP_003152868.1	ComE operon protein 2	-
MKW31_RS12315 (MKW31_12295)	comEA	2579319..2579933 (-)	615	WP_032866693.1	helix-hairpin-helix domain-containing protein	Machinery gene
MKW31_RS12320 (MKW31_12300)	comER	2579992..2580813 (+)	822	WP_242750419.1	late competence protein ComER	-
MKW31_RS12325 (MKW31_12305)	-	2580882..2581619 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
MKW31_RS12330 (MKW31_12310)	rsfS	2581616..2581972 (-)	357	WP_007408260.1	ribosome silencing factor	-
MKW31_RS12335 (MKW31_12315)	yqeK	2581990..2582550 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MKW31_RS12340 (MKW31_12320)	-	2582540..2583109 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
MKW31_RS12345 (MKW31_12325)	yhbY	2583120..2583410 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
MKW31_RS12350 (MKW31_12330)	aroE	2583404..2584246 (-)	843	WP_033574150.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22151.28 Da
• Isoelectric Point: 6.4858

>NTDB_id=659853 MKW31_RS12315 WP_032866693.1 2579319..2579933(-) (comEA) [Bacillus velezensis strain 37-1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=659853 MKW31_RS12315 WP_032866693.1 2579319..2579933(-) (comEA) [Bacillus velezensis strain 37-1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTTAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCATACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363