Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SRAA_RS08230 Genome accession   NZ_AP014568
Coordinates   1757936..1759666 (+) Length   576 a.a.
NCBI ID   WP_045532035.1    Uniprot ID   A0A060NJ55
Organism   Serpentinimonas raichei strain A1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1752936..1764666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SRAA_RS08205 (SRAA_1638) - 1753026..1753913 (-) 888 WP_045532029.1 carbohydrate kinase family protein -
  SRAA_RS08210 (SRAA_1639) - 1753932..1754921 (-) 990 WP_045532031.1 DUF3426 domain-containing protein -
  SRAA_RS08215 (SRAA_1640) prmA 1754947..1755831 (-) 885 WP_045533565.1 50S ribosomal protein L11 methyltransferase -
  SRAA_RS08220 (SRAA_1641) accC 1755944..1757293 (-) 1350 WP_045532033.1 acetyl-CoA carboxylase biotin carboxylase subunit -
  SRAA_RS08225 (SRAA_1642) accB 1757307..1757780 (-) 474 WP_045532034.1 acetyl-CoA carboxylase biotin carboxyl carrier protein -
  SRAA_RS08230 (SRAA_1643) pilB 1757936..1759666 (+) 1731 WP_045532035.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SRAA_RS08235 (SRAA_1644) pilC 1759708..1760925 (+) 1218 WP_045533567.1 type II secretion system F family protein Machinery gene
  SRAA_RS12310 (SRAA_1645) hemP 1761092..1761274 (+) 183 WP_144318741.1 hemin uptake protein HemP -
  SRAA_RS08245 (SRAA_1646) - 1761431..1763740 (+) 2310 WP_045532040.1 TonB-dependent hemoglobin/transferrin/lactoferrin family receptor -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62474.86 Da        Isoelectric Point: 6.1174

>NTDB_id=65928 SRAA_RS08230 WP_045532035.1 1757936..1759666(+) (pilB) [Serpentinimonas raichei strain A1]
MIDTEAPVSSMALPGLARALVAAGTLTQQSAEGLYAKAQAHKSSFIAELVGSGALSASDLAHTMSSTFAVPLIDLDAIDV
QRLPKGVLDPKFAKQYRIVVLSRRGNRISVATADPTHTEVAEKIKFTTQLSVDWVIAELDKLGKLIDSTSASASEAIEHI
VGGDFEFDDGAIELATAESSEKVSAEVEDAPVVRFLHKMLLDAYTMRASDLHFEPYEHFYRVRFRIDGELREIASPPVAL
KEKLASRIKVISKLDIAEKRVPQDGRMKLKVGPDKSIDFRVSTLPTLFGEKIVIRILDPSSAQMGIDALGYEADEKALLL
EAISRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGVNISTAEDPSEINLPGVNQVNVNEKAGLTFAAALKSFLRQDPDII
MVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPGTLTRLINMGVLPFNIASSVNLVTAQRLARRLCKQCKQPHEDLPEL
ALLEAGFKPEELDGSWKPYRPVGCPACHNGYKGRLGIYQVMPISEAMQHIILRGGSALEIQAQARREGIRTLRESGLRKV
KLGLTSLEEVLGCTND

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=65928 SRAA_RS08230 WP_045532035.1 1757936..1759666(+) (pilB) [Serpentinimonas raichei strain A1]
ATGATCGACACCGAAGCCCCCGTCTCCTCTATGGCCTTGCCAGGGCTGGCGCGCGCCTTGGTGGCCGCCGGCACCCTGAC
CCAGCAAAGCGCCGAAGGTCTGTACGCCAAGGCCCAGGCCCACAAAAGCAGCTTCATTGCCGAACTGGTGGGCAGCGGCG
CCTTGAGCGCCAGCGATTTGGCGCACACCATGTCGAGCACCTTTGCCGTGCCGCTGATCGACCTCGACGCCATCGACGTG
CAGCGTCTGCCCAAAGGCGTGCTCGACCCCAAGTTTGCCAAGCAATACCGCATTGTGGTGTTGAGCCGCCGTGGCAACCG
CATCTCAGTGGCCACGGCCGACCCCACCCACACCGAAGTGGCTGAAAAAATCAAGTTCACCACCCAGCTTTCGGTGGACT
GGGTGATCGCCGAACTCGACAAACTGGGCAAATTGATCGACAGCACCAGCGCCAGCGCCAGCGAAGCGATCGAACACATC
GTGGGCGGCGATTTTGAATTCGACGACGGCGCGATTGAATTGGCTACCGCCGAGAGCAGCGAGAAAGTCTCGGCCGAGGT
CGAAGACGCGCCGGTGGTGCGTTTTTTGCACAAAATGCTGCTCGATGCCTACACGATGCGGGCATCCGACCTGCATTTCG
AGCCCTATGAGCACTTTTACCGGGTGCGCTTTCGCATCGACGGTGAGCTGCGCGAAATCGCCTCCCCACCGGTGGCCCTA
AAAGAAAAGCTGGCTTCGCGCATCAAAGTCATCTCAAAACTCGATATCGCAGAAAAACGCGTGCCGCAAGACGGGCGCAT
GAAACTCAAGGTCGGGCCCGATAAATCGATCGATTTTCGCGTCAGCACCCTGCCCACGCTGTTTGGCGAAAAGATCGTCA
TCCGCATTCTGGACCCGAGCAGCGCCCAAATGGGCATCGACGCGCTGGGCTACGAAGCTGACGAAAAGGCCCTGCTGCTG
GAGGCCATCAGCCGCCCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGATCGGGCAAAACGGTGTCGCTCTACACCTG
CCTCAACATCCTGAATCAGCCCGGAGTCAACATCTCGACCGCCGAAGACCCCTCGGAGATCAACCTGCCCGGGGTGAATC
AGGTCAACGTCAACGAGAAGGCGGGCCTCACATTCGCGGCGGCGCTCAAGTCGTTTTTGCGCCAAGACCCCGACATCATC
ATGGTCGGAGAAATCCGCGACCTCGAAACCGCCGACATCGCCATCAAGGCGGCCCAGACCGGCCACTTGGTGCTCTCGAC
CTTGCACACCAACGACGCCCCCGGCACCCTGACGCGCCTGATCAATATGGGTGTGCTGCCGTTCAACATCGCCTCCAGCG
TGAACTTGGTCACGGCGCAGCGGCTGGCGCGGCGACTGTGCAAACAATGCAAGCAACCGCACGAAGATTTGCCCGAACTG
GCGTTGCTGGAGGCCGGCTTCAAGCCCGAAGAGCTCGACGGCAGTTGGAAACCCTACCGTCCCGTGGGCTGCCCGGCCTG
CCACAATGGCTACAAGGGGCGGCTTGGCATTTATCAGGTGATGCCGATCAGCGAAGCCATGCAGCACATTATTCTGCGCG
GCGGTAGTGCGCTCGAGATTCAAGCCCAAGCGCGCCGCGAAGGCATTCGCACTTTGCGCGAATCGGGGCTGCGCAAGGTT
AAACTGGGCCTTACTTCCCTCGAGGAAGTGTTGGGCTGCACCAACGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A060NJ55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.439

98.958

0.549
  pilB Acinetobacter baylyi ADP1

54.912

98.958

0.543
  pilF Neisseria gonorrhoeae MS11

54.255

97.917

0.531
  pilB Legionella pneumophila strain ERS1305867

49.649

98.958

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

47.331

97.569

0.462
  pilB Vibrio campbellii strain DS40M4

46.466

98.264

0.457
  pilB Vibrio cholerae strain A1552

45.534

99.132

0.451
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.035

93.924

0.385
  pilF Thermus thermophilus HB27

39.153

98.438

0.385

Multiple sequence alignment