Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ATS73_RS12475 Genome accession   NZ_CP092598
Coordinates   2628898..2630574 (+) Length   558 a.a.
NCBI ID   WP_058429669.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. H100     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2623898..2635574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ATS73_RS12450 (ATS73_012375) - 2623939..2624358 (+) 420 WP_058429673.1 pilin -
  ATS73_RS12455 (ATS73_012380) - 2624677..2625762 (+) 1086 WP_235591613.1 O-antigen ligase family protein -
  ATS73_RS12460 (ATS73_012385) - 2625851..2627079 (+) 1229 Protein_2386 type II secretion system F family protein -
  ATS73_RS12465 (ATS73_012390) pilD 2627223..2628116 (+) 894 WP_058429671.1 A24 family peptidase Machinery gene
  ATS73_RS12470 (ATS73_012395) coaE 2628134..2628765 (+) 632 Protein_2388 dephospho-CoA kinase -
  ATS73_RS12475 (ATS73_012400) pilB 2628898..2630574 (+) 1677 WP_058429669.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ATS73_RS12480 (ATS73_012405) yacG 2630642..2630872 (+) 231 WP_006794561.1 DNA gyrase inhibitor YacG -
  ATS73_RS12485 (ATS73_012410) - 2630955..2631446 (-) 492 WP_058429668.1 Spy/CpxP family protein refolding chaperone -
  ATS73_RS12490 (ATS73_012415) - 2631630..2632319 (+) 690 Protein_2392 response regulator -
  ATS73_RS12495 (ATS73_012420) - 2632297..2633712 (+) 1416 WP_058429666.1 ATP-binding protein -
  ATS73_RS12500 (ATS73_012425) - 2633716..2634663 (+) 948 WP_058429665.1 alpha/beta hydrolase -
  ATS73_RS12505 (ATS73_012430) - 2634623..2635472 (-) 850 Protein_2395 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61943.08 Da        Isoelectric Point: 5.1577

>NTDB_id=659235 ATS73_RS12475 WP_058429669.1 2628898..2630574(+) (pilB) [Pseudoalteromonas sp. H100]
MNINSPLLRKFLTLGRIDADTVKAKQQEFASTAELISKCGKIDSKELAEQCIDLFRVPYFDLKDFDPASIPADLVKEKLI
RKHHILPLVQKGRKVYVAASDPTDYGAFENFEFSTGLSCEIVVVDYIQLDNKIEQLLDATGSLDLSDDEFKEFADLDTES
QKESAPQDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLATKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEEEQKNLYMDALGQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINPKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTAEQLPSEELSRQGFSKAQIEDMTL
YAPKGCDGCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLKAGFNNLRLSGLRKAADGLTSLAEINRVTNM

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=659235 ATS73_RS12475 WP_058429669.1 2628898..2630574(+) (pilB) [Pseudoalteromonas sp. H100]
ATGAATATTAACTCACCGTTATTAAGAAAGTTTCTTACGCTTGGGCGTATTGATGCCGATACGGTAAAAGCAAAGCAGCA
AGAGTTTGCCTCAACCGCTGAACTGATTTCTAAATGCGGAAAAATAGACAGTAAAGAGTTAGCAGAGCAATGTATTGATT
TGTTTCGTGTTCCCTATTTTGATTTAAAAGACTTTGACCCTGCGTCTATTCCTGCCGATCTTGTAAAAGAAAAACTGATC
CGCAAACACCATATTTTACCGTTGGTACAAAAAGGCCGAAAAGTGTATGTGGCAGCCTCTGATCCAACCGATTACGGCGC
CTTCGAAAACTTTGAATTTAGTACCGGTCTTTCATGCGAAATAGTCGTCGTTGATTACATTCAGCTCGATAATAAAATTG
AGCAATTGCTAGATGCAACCGGCAGCCTAGATTTGAGCGATGATGAGTTTAAAGAATTTGCCGACCTAGACACAGAAAGC
CAAAAAGAATCTGCACCGCAAGATGACGACAAAGACGATGCACCAATTATTGTGTACATTAATAAAATTTTAATGGACGC
GATAAAAAAAGGCGCATCGGATCTACACTTTGAACCTTACGAGCATAAGTACCGAATTCGTTTTCGTATTGATGGCATCT
TGCACGAAGTGGCAAGTCCACCTAATACATTAGCAACCAAGCTTTCTGCTCGTATTAAAGTAATGTCGCGACTTGATATT
GCCGAAAAGCGTAAACCACAAGATGGCCGAATTAAATTAAAAATTACTGAGCGAAAAAGTATCGACTTTCGTGTCAGTAC
TATGCCCACGCTGTGGGGCGAAAAAATAGTAATGCGTATTCTTGATTCATCAAGCGCTATGCTAGGTATTGATGTACTTG
GTTACGAAGAAGAGCAGAAAAATCTATACATGGATGCGCTTGGTCAACCACAAGGCATGATACTCGTTACAGGTCCTACC
GGGTCAGGTAAAACGGTATCGCTTTATACGGGTTTAAACATATTAAACCAACCTGAGCGAAACATTAGTACCGCTGAGGA
TCCGGTTGAGATAAACCTTGAAGGGATAAACCAAGTACAAATTAACCCTAAAGCCGATATGACCTTTGCTAATGCATTGC
GTGCGTTTTTACGTCAAGATCCCGATGTGGTTATGGTAGGTGAAATACGTGACCTTGAAACCGCAGAAATTTCAATAAAA
GCGGCGCAAACAGGTCACTTGGTTTTATCTACGTTACATACCAATTCAGCGCCAGAAACCATCACCCGACTATTAAATAT
GGGTGTACCCGCCTATAACGTAGCCAGTTCAATTAGTTTAATTATTGCACAGCGATTAGCACGCCGTTTATGTCCAAAAT
GTAAAACGGCAGAACAATTGCCAAGCGAGGAGCTCTCTCGCCAAGGATTTAGCAAAGCGCAAATCGAAGATATGACTCTA
TATGCACCTAAAGGGTGTGATGGCTGTACCGATGGTTACAAAGGCCGTGTAGGTATTTACGAAGTTATGCAAATCACACC
AGAAATTGCACAAATAATCATGCGCGGTGGTAACTCACTAGAAATCGCTGAGGTATCATTAAAAGCCGGTTTTAATAATT
TGCGCCTATCGGGTTTACGTAAAGCCGCTGATGGATTAACGTCACTTGCTGAAATAAACCGCGTAACTAATATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.81

100

0.568
  pilB Acinetobacter baylyi ADP1

58.416

90.502

0.529
  pilB Legionella pneumophila strain ERS1305867

52.865

96.953

0.513
  pilB Vibrio cholerae strain A1552

53.571

95.341

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

53.008

95.341

0.505
  pilB Vibrio campbellii strain DS40M4

52.354

95.161

0.498
  pilF Neisseria gonorrhoeae MS11

49.808

93.548

0.466
  pilB/pilB1 Synechocystis sp. PCC 6803

37.621

100

0.419
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.973

94.265

0.367