Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NEJAP_RS04980 Genome accession   NZ_AP014546
Coordinates   1094832..1096544 (-) Length   570 a.a.
NCBI ID   WP_201349575.1    Uniprot ID   A0A7R6PIK9
Organism   Neptunomonas japonica JAMM 1380     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1089832..1101544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NEJAP_RS04955 (NEJAP_0968) - 1090843..1091775 (+) 933 WP_329610935.1 Nudix family hydrolase -
  NEJAP_RS04960 (NEJAP_0969) - 1091756..1091965 (-) 210 WP_201349572.1 DNA gyrase inhibitor YacG -
  NEJAP_RS04965 (NEJAP_0970) coaE 1091962..1092576 (-) 615 WP_201349573.1 dephospho-CoA kinase -
  NEJAP_RS04970 (NEJAP_0971) pilD 1092644..1093510 (-) 867 WP_201349574.1 prepilin peptidase Machinery gene
  NEJAP_RS04975 - 1093572..1094794 (-) 1223 Protein_987 type II secretion system F family protein -
  NEJAP_RS04980 (NEJAP_0974) pilB 1094832..1096544 (-) 1713 WP_201349575.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NEJAP_RS19520 - 1096908..1097022 (+) 115 Protein_989 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NEJAP_RS04985 (NEJAP_0975) - 1097043..1097354 (+) 312 WP_419197848.1 pilin -
  NEJAP_RS04990 (NEJAP_0976) - 1097685..1098032 (+) 348 WP_201349577.1 hypothetical protein -
  NEJAP_RS04995 (NEJAP_0977) - 1098111..1098500 (-) 390 WP_201349578.1 3-phosphoshikimate 1-carboxyvinyltransferase -
  NEJAP_RS05000 (NEJAP_0978) nadC 1098533..1099387 (-) 855 WP_201349579.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NEJAP_RS05005 (NEJAP_0979) ampD 1099507..1100082 (+) 576 WP_201349580.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NEJAP_RS05010 (NEJAP_0980) - 1100218..1101267 (+) 1050 WP_201349581.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62935.12 Da        Isoelectric Point: 5.3395

>NTDB_id=65879 NEJAP_RS04980 WP_201349575.1 1094832..1096544(-) (pilB) [Neptunomonas japonica JAMM 1380]
MQVQQPLSGLARRLVKDGVFSADVARNAIEKARASQKPFISHIIDERLVTAHRAASAASDEFGLPLLDLNCINTESIPQG
LVADNLISKHHALPLVKRDNRLYVALADPTNIQALDEFKFHTGITTEGVIVEEDKLKQLIEAFLDDQGSALEELDDTDLD
SLEIDDGSSKEEEEQTSEDASKDAPIVRFVNKILLDCIKKGASDIHFEPYEKAYRIRSRIDGILQEIAKPPNNLAKRLSA
RLKVMSQMDISERRMPQDGRIKMKISKNRAIDFRVNTLPTLWGEKIVLRILDPSSAKMGVDALGFSQMQNDLYMSTLHKP
QGMILVTGPTGSGKTVTLYTGLNILNTPELNISTAEDPVEINLEGINQVHVNAKVGLSFAEALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVSLIIAQRLARRLCEPCKKPASVPEAALIEEGF
SPEQLRSIDLYEANPEGCPKCTRGYKGRVGIYEMLPMTPEIAEVIMNNGSSIDIIRIAQEQGFRPLRQTGLDKVAQGLTS
LDEMNRVIKT

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=65879 NEJAP_RS04980 WP_201349575.1 1094832..1096544(-) (pilB) [Neptunomonas japonica JAMM 1380]
ATGCAGGTTCAGCAACCTTTAAGCGGCTTGGCAAGAAGACTGGTTAAAGATGGAGTTTTTTCGGCTGATGTAGCGCGCAA
CGCCATTGAAAAAGCCCGCGCCTCTCAAAAACCCTTTATCAGTCATATAATTGATGAGCGTTTAGTGACTGCCCACCGTG
CGGCAAGTGCAGCATCTGATGAATTTGGCCTTCCATTACTCGACCTTAACTGCATAAACACTGAATCTATCCCTCAAGGA
CTGGTTGCTGATAATTTAATTTCCAAGCATCACGCCCTCCCTTTAGTAAAGCGTGATAACCGGCTTTATGTTGCATTAGC
TGATCCTACGAATATCCAAGCACTCGATGAGTTTAAATTCCACACGGGAATAACCACCGAAGGCGTCATCGTTGAAGAAG
ATAAGCTCAAGCAGCTTATTGAGGCTTTCCTAGATGACCAAGGCTCCGCTCTTGAAGAATTGGATGACACAGATTTAGAT
TCTCTAGAGATAGATGACGGCAGCAGCAAAGAAGAAGAGGAACAAACCAGTGAGGATGCTTCTAAAGATGCTCCTATTGT
ACGGTTTGTGAATAAAATCTTACTAGACTGCATTAAAAAAGGTGCATCAGATATACACTTTGAACCCTACGAAAAAGCTT
ACCGAATTCGTAGCCGTATCGATGGTATTTTACAAGAGATAGCCAAGCCGCCTAACAACCTTGCCAAACGCTTGTCAGCC
CGCTTAAAAGTCATGTCACAAATGGATATTTCTGAACGACGCATGCCGCAAGATGGGCGCATAAAAATGAAAATATCAAA
GAACCGCGCTATTGATTTTCGTGTGAATACACTCCCCACACTCTGGGGCGAAAAAATAGTTTTACGTATTTTGGACCCTT
CCAGCGCTAAAATGGGAGTAGATGCTTTAGGCTTTAGCCAAATGCAAAACGACCTGTATATGAGCACACTGCATAAGCCG
CAAGGAATGATACTAGTTACTGGTCCTACCGGTAGCGGTAAAACAGTAACCCTCTATACAGGGCTTAATATTCTTAATAC
TCCTGAGTTAAACATCTCTACTGCCGAAGATCCTGTCGAAATAAACCTTGAAGGAATAAACCAAGTACATGTTAACGCCA
AAGTCGGTTTAAGCTTTGCAGAAGCTTTGCGCTCGTTCTTGCGACAAGACCCTGATGTTGTCATGGTAGGGGAAATACGT
GATTTGGAAACAGCCGAAATTGCCATAAAAGCGGCTCAAACCGGGCATATGGTTCTATCAACCCTGCACACCAATAGCGC
CCCTGAAACATTAACACGTTTACGTAATATGGGTGTTGCATCATTCAACATTGCAACCTCAGTTAGCTTAATTATTGCAC
AACGCCTTGCACGTCGTTTATGCGAACCCTGTAAAAAACCTGCATCTGTGCCAGAAGCTGCTTTAATAGAAGAAGGGTTT
TCTCCAGAACAGCTTCGTAGTATTGATCTATATGAAGCAAACCCAGAGGGATGCCCTAAATGTACCAGAGGCTATAAAGG
TCGTGTTGGTATCTATGAAATGCTACCCATGACGCCAGAAATAGCTGAAGTCATTATGAACAACGGCTCCTCTATAGATA
TTATCCGTATTGCTCAAGAGCAAGGTTTTAGGCCACTTCGCCAAACAGGGTTAGATAAAGTAGCACAAGGTCTTACTAGC
TTGGATGAAATGAATCGCGTTATTAAGACCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7R6PIK9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.576

98.421

0.567
  pilB Acinetobacter baylyi ADP1

57.398

98.421

0.565
  pilB Legionella pneumophila strain ERS1305867

54.754

99.649

0.546
  pilB Vibrio cholerae strain A1552

51.579

100

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.767

99.298

0.514
  pilB Vibrio campbellii strain DS40M4

50.794

99.474

0.505
  pilF Neisseria gonorrhoeae MS11

47.611

99.123

0.472
  pilB/pilB1 Synechocystis sp. PCC 6803

41.221

91.93

0.379

Multiple sequence alignment