Detailed information NTG0658194

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MJ920_RS12585
• Genome accession: NZ_CP092499
• Coordinates: 2583685..2584299 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain YC_89
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2578685..2589299

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MJ920_RS12555 (MJ920_12550)	rpsT	2578984..2579250 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
MJ920_RS12560 (MJ920_12555)	holA	2579267..2580308 (-)	1042	Protein_2429	DNA polymerase III subunit delta	-
MJ920_RS12565 (MJ920_12560)	-	2580348..2580502 (-)	155	Protein_2430	hypothetical protein	-
MJ920_RS12570 (MJ920_12565)	-	2580543..2580677 (+)	135	WP_003152870.1	YqzM family protein	-
MJ920_RS12575 (MJ920_12570)	-	2580696..2583048 (-)	2353	Protein_2432	DNA internalization-related competence protein ComEC/Rec2	-
MJ920_RS12580 (MJ920_12575)	-	2583049..2583618 (-)	570	WP_003152868.1	ComE operon protein 2	-
MJ920_RS12585 (MJ920_12580)	comEA	2583685..2584299 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
MJ920_RS12590 (MJ920_12585)	comER	2584358..2585179 (+)	822	WP_043867298.1	late competence protein ComER	-
MJ920_RS12595 (MJ920_12590)	-	2585248..2585985 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
MJ920_RS12600 (MJ920_12595)	rsfS	2585982..2586338 (-)	357	WP_003152864.1	ribosome silencing factor	-
MJ920_RS12605 (MJ920_12600)	yqeK	2586335..2586916 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MJ920_RS12610 (MJ920_12605)	-	2586906..2587475 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
MJ920_RS12615 (MJ920_12610)	yhbY	2587486..2587776 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
MJ920_RS12620 (MJ920_12615)	aroE	2587770..2588612 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=658194 MJ920_RS12585 WP_043867297.1 2583685..2584299(-) (comEA) [Bacillus velezensis strain YC_89]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=658194 MJ920_RS12585 WP_043867297.1 2583685..2584299(-) (comEA) [Bacillus velezensis strain YC_89]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363