Detailed information NTG0657701

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MJX77_RS01060
• Genome accession: NZ_CP092454
• Coordinates: 234445..235563 (+)
• Length: 372 a.a.
• NCBI ID: WP_013198760.1
• Uniprot ID: A0AAN0UEB6
• Organism: Acinetobacter sp. AHP123
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 229445..240563

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MJX77_RS01045	-	231619..232461 (-)	843	WP_013198758.1	undecaprenyl-diphosphate phosphatase	-
MJX77_RS01050	-	232569..233261 (-)	693	WP_252060370.1	YggS family pyridoxal phosphate-dependent enzyme	-
MJX77_RS01055	pilT	233388..234425 (+)	1038	WP_003653239.1	type IV pilus twitching motility protein PilT	Machinery gene
MJX77_RS01060	pilU	234445..235563 (+)	1119	WP_013198760.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MJX77_RS01065	fur	235633..236070 (-)	438	WP_004794120.1	ferric iron uptake transcriptional regulator	-
MJX77_RS01070	bamE	236182..236580 (+)	399	WP_003653245.1	outer membrane protein assembly factor BamE	-
MJX77_RS01075	-	236645..236959 (-)	315	WP_252060371.1	RnfH family protein	-
MJX77_RS01080	-	236959..238026 (-)	1068	WP_252060372.1	hypothetical protein	-
MJX77_RS01085	-	238178..238633 (-)	456	WP_013198763.1	bacteriohemerythrin	-
MJX77_RS01090	-	238885..239733 (-)	849	WP_252060373.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41887.10 Da
• Isoelectric Point: 6.6401

>NTDB_id=657701 MJX77_RS01060 WP_013198760.1 234445..235563(+) (pilU) [Acinetobacter sp. AHP123]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNTKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=657701 MJX77_RS01060 WP_013198760.1 234445..235563(+) (pilU) [Acinetobacter sp. AHP123]
ATGGATTTTAATGACTTACTCAACCTCATGGTTGAAAAAAAATCATCCGATCTTTTTATTACAGATGGTGTTGCACCATC
TATGAAGATTAACGGACAAATTGTTCCAATTTCAAAGAATAGTCTTTCAGGTGAGGTTATTGGTCAACTCTTACATTCCA
TCATGAGCGAAAAACAGCGCAAAGAATTTGCGGACACTCGTGAATGTAACTTTGCCATTATGAACCGCGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAGCGTGATATGCCGGGCATGGTACTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTTCTTAAAGATTTATCAATGACCAAACGCGGCATCATTATTTTTGTTGGTGCGACAG
GTACAGGTAAATCAACGTCTTTGGCTTCAATGATTAGCTATCGTAACCACAATACTAAAGGTCATATCATTACCATTGAA
GACCCAATCGAGTTTATTCACGAACATGCGGGCTGCATCATTACCCAACGTGAAGTCGGAATCGATACGGACTCATTTGA
AATTGCCTTAAAAAATACTTTGCGACAAGCACCAGATGTCATCTTGATTGGTGAGATTCGATCTCGTGAAGTTATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTGGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATCATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTACATGGACTTATCTCTTAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACACCTGATGGCAACTCTCGCCGCGCCGCAATTGAAATTTTAATTAACTCACCATTAATTTCAGATT
ACATTCGTAAAGGTGAAATTCATGAAATTAAAGATTTAATGAAACGATCACGTGAACTCGGTATGCAAACCTTCGACCAA
GCATTGTTTGATCTCTATAAAGCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCTGACTCACCAAATGATTTACG
TTTGACGATTAAGCTTGCTGAAGAAGGACCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	74.868	100	0.761
pilU	Pseudomonas stutzeri DSM 10701	67.925	99.731	0.677
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.659	97.849	0.398
pilT	Legionella pneumophila strain Lp02	39.645	90.86	0.36
pilT	Legionella pneumophila strain ERS1305867	39.645	90.86	0.36