Detailed information NTG0657450

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MG974_RS12165
• Genome accession: NZ_CP092439
• Coordinates: 2510403..2511017 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 504
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2505403..2516017

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MG974_RS12135 (MG974_12135)	rpsT	2505703..2505969 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
MG974_RS12140 (MG974_12140)	holA	2505986..2507027 (-)	1042	Protein_2347	DNA polymerase III subunit delta	-
MG974_RS12145 (MG974_12145)	-	2507067..2507221 (-)	155	Protein_2348	hypothetical protein	-
MG974_RS12150 (MG974_12150)	-	2507262..2507396 (+)	135	WP_003152870.1	YqzM family protein	-
MG974_RS12155 (MG974_12155)	comEC	2507415..2509766 (-)	2352	WP_043867296.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MG974_RS12160 (MG974_12160)	-	2509767..2510336 (-)	570	WP_003152868.1	ComE operon protein 2	-
MG974_RS12165 (MG974_12165)	comEA	2510403..2511017 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
MG974_RS12170 (MG974_12170)	comER	2511076..2511897 (+)	822	WP_044053476.1	late competence protein ComER	-
MG974_RS12175 (MG974_12175)	-	2511966..2512703 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
MG974_RS12180 (MG974_12180)	rsfS	2512700..2513056 (-)	357	WP_003152864.1	ribosome silencing factor	-
MG974_RS12185 (MG974_12185)	yqeK	2513053..2513634 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MG974_RS12190 (MG974_12190)	-	2513624..2514193 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
MG974_RS12195 (MG974_12195)	yhbY	2514204..2514494 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
MG974_RS12200 (MG974_12200)	aroE	2514488..2515330 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=657450 MG974_RS12165 WP_043867297.1 2510403..2511017(-) (comEA) [Bacillus velezensis strain 504]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=657450 MG974_RS12165 WP_043867297.1 2510403..2511017(-) (comEA) [Bacillus velezensis strain 504]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363