Detailed information NTG0657248

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MH925_RS12210
• Genome accession: NZ_CP092412
• Coordinates: 2513268..2513882 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus velezensis strain HNU24
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2508268..2518882

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MH925_RS12180	rpsT	2508571..2508837 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
MH925_RS12185	holA	2508854..2509895 (-)	1042	Protein_2348	DNA polymerase III subunit delta	-
MH925_RS12190	-	2509935..2510086 (-)	152	Protein_2349	hypothetical protein	-
MH925_RS12195	-	2510127..2510261 (+)	135	WP_003152870.1	YqzM family protein	-
MH925_RS12200	comEC	2510280..2512631 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MH925_RS12205	-	2512632..2513201 (-)	570	WP_003152868.1	ComE operon protein 2	-
MH925_RS12210	comEA	2513268..2513882 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
MH925_RS12215	comER	2513941..2514762 (+)	822	WP_012118027.1	late competence protein ComER	-
MH925_RS12220	-	2514831..2515568 (-)	738	WP_012118028.1	class I SAM-dependent DNA methyltransferase	-
MH925_RS12225	rsfS	2515565..2515921 (-)	357	WP_007408260.1	ribosome silencing factor	-
MH925_RS12230	yqeK	2515938..2516498 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MH925_RS12235	-	2516488..2517057 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
MH925_RS12240	yhbY	2517068..2517358 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
MH925_RS12245	aroE	2517352..2518194 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=657248 MH925_RS12210 WP_012118026.1 2513268..2513882(-) (comEA) [Bacillus velezensis strain HNU24]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=657248 MH925_RS12210 WP_012118026.1 2513268..2513882(-) (comEA) [Bacillus velezensis strain HNU24]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363