Detailed information NTG0657047

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MID02_RS12615
• Genome accession: NZ_CP092383
• Coordinates: 2634192..2634806 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain SF327
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2629192..2639806

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MID02_RS12585 (MID02_12585)	rpsT	2629492..2629758 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
MID02_RS12590 (MID02_12590)	holA	2629775..2630816 (-)	1042	Protein_2439	DNA polymerase III subunit delta	-
MID02_RS12595 (MID02_12595)	-	2630856..2631010 (-)	155	Protein_2440	hypothetical protein	-
MID02_RS12600 (MID02_12600)	-	2631051..2631185 (+)	135	WP_003152870.1	YqzM family protein	-
MID02_RS12605 (MID02_12605)	comEC	2631204..2633555 (-)	2352	WP_239819197.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MID02_RS12610 (MID02_12610)	-	2633556..2634125 (-)	570	WP_003152868.1	ComE operon protein 2	-
MID02_RS12615 (MID02_12615)	comEA	2634192..2634806 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
MID02_RS12620 (MID02_12620)	comER	2634865..2635686 (+)	822	WP_115996552.1	late competence protein ComER	-
MID02_RS12625 (MID02_12625)	-	2635755..2636492 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
MID02_RS12630 (MID02_12630)	rsfS	2636489..2636845 (-)	357	WP_239819198.1	ribosome silencing factor	-
MID02_RS12635 (MID02_12635)	yqeK	2636842..2637423 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MID02_RS12640 (MID02_12640)	-	2637413..2637982 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
MID02_RS12645 (MID02_12645)	yhbY	2637993..2638283 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
MID02_RS12650 (MID02_12650)	aroE	2638277..2639119 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=657047 MID02_RS12615 WP_043867297.1 2634192..2634806(-) (comEA) [Bacillus velezensis strain SF327]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=657047 MID02_RS12615 WP_043867297.1 2634192..2634806(-) (comEA) [Bacillus velezensis strain SF327]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363