Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MIH17_RS03975 Genome accession   NZ_CP092288
Coordinates   829586..831703 (+) Length   705 a.a.
NCBI ID   WP_249007653.1    Uniprot ID   -
Organism   Marinobacter sp. M2C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 824586..836703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIH17_RS03955 (MIH17_03955) pilM 826773..827837 (+) 1065 WP_249007649.1 pilus assembly protein PilM Machinery gene
  MIH17_RS03960 (MIH17_03960) - 827837..828403 (+) 567 WP_249007650.1 PilN domain-containing protein -
  MIH17_RS03965 (MIH17_03965) pilO 828400..829011 (+) 612 WP_249007651.1 type 4a pilus biogenesis protein PilO -
  MIH17_RS03970 (MIH17_03970) - 829014..829586 (+) 573 WP_249007652.1 pilus assembly protein PilP -
  MIH17_RS03975 (MIH17_03975) pilQ 829586..831703 (+) 2118 WP_249007653.1 type IV pilus secretin PilQ Machinery gene
  MIH17_RS03980 (MIH17_03980) aroK 831843..832409 (+) 567 WP_249007654.1 shikimate kinase AroK -
  MIH17_RS03985 (MIH17_03985) aroB 832402..833493 (+) 1092 WP_249007655.1 3-dehydroquinate synthase -
  MIH17_RS03990 (MIH17_03990) - 833519..835117 (+) 1599 WP_249007656.1 AAA family ATPase -

Sequence

Protein


Download         Length: 705 a.a.        Molecular weight: 75425.99 Da        Isoelectric Point: 4.3801

>NTDB_id=656533 MIH17_RS03975 WP_249007653.1 829586..831703(+) (pilQ) [Marinobacter sp. M2C]
MNNSNMNAQKCLKPRLAMFKKLNVIVCAVTVGLLSSLASAATLQDVSFSSLPGDRLEVTLQFDGPPPEPTGYTIERPARI
AVDLKDTTSALGARSLPLGSGNAQSMTVVETNDRTRLIFNLIELVPYTTQRNNNALVMIIGGDSADLASGVSGSDGASPV
ASAADAAPALVDVDFRRGAQGEGRVVVRLASATTQVDLSELGGKIRLTMPDLTVPERLRRRLDVTDFATPVQRIDTFIEG
GGAVIEIRPEGDYDYIAYQSGSEFTVSVERLSEEEAVTRREEKFPYSGDKLSLNFQDIEVRSVLQLIADFTGLNLVASDT
VGGSITLRLQNVPWDQALDLILKTKGLDKRQIGNVLLVAPADEIAARERLELETTKQIAELAPVRLDIIQVNYAKAAEVV
ALVQADQELISSRGFISSDDRTNTISVRETIEKLDEIRRLVSTWDVPVRQVSIEARIVRAQTNVAESLGVSWGGAAYSVS
GNNVISVGGSQSAVEEARNAATGGSGTITFPGALAVDLGVSGDGASSFAIGFGSDDFLVDLELSALESDGKAEIVSQPRV
VTADRQTASIKSGQEIPYQEASSSGATSTSFKEAVLSLEVTPQITPDDKIIMDLEVTQDSRGEDTPSGPAINTNSVTTQV
LVANGETIVLGGIFESTNTETTTKTPFLGDIPYLGRLFKRTQTTELRSELLIFITPKIIKNDLVR

Nucleotide


Download         Length: 2118 bp        

>NTDB_id=656533 MIH17_RS03975 WP_249007653.1 829586..831703(+) (pilQ) [Marinobacter sp. M2C]
ATGAATAACAGCAACATGAATGCACAAAAATGCTTGAAGCCGAGGCTAGCGATGTTCAAAAAACTCAACGTAATTGTCTG
TGCGGTAACCGTTGGGTTGTTGTCCAGCCTGGCCAGCGCTGCCACTTTGCAGGATGTGTCGTTTTCATCTTTACCTGGGG
ATCGGCTGGAAGTGACTTTGCAGTTTGACGGTCCACCGCCTGAACCTACGGGTTACACCATTGAGCGCCCCGCTCGTATT
GCGGTAGACCTGAAAGACACCACCAGTGCCCTGGGTGCCCGCAGCTTGCCGTTGGGCAGCGGGAATGCGCAAAGCATGAC
CGTTGTTGAAACCAACGACCGTACCCGCCTTATTTTCAACCTGATTGAGCTGGTTCCTTACACCACTCAGCGCAATAATA
ACGCGTTGGTGATGATTATTGGCGGCGATTCCGCAGATTTAGCCAGTGGCGTTTCGGGATCTGACGGTGCCAGCCCTGTT
GCTTCGGCAGCGGACGCGGCGCCGGCGTTGGTAGACGTCGATTTTCGTCGCGGTGCACAAGGCGAAGGCCGTGTGGTTGT
GCGGCTGGCCAGTGCGACGACTCAGGTTGACCTGAGCGAACTGGGCGGAAAAATCAGACTGACCATGCCGGATCTGACTG
TGCCCGAGCGCCTGCGTCGCCGTTTAGACGTGACAGATTTTGCCACGCCGGTACAACGGATCGACACCTTTATTGAGGGT
GGCGGTGCGGTTATAGAAATTCGTCCGGAAGGCGACTATGACTACATTGCGTACCAGTCTGGCAGCGAATTTACCGTGAG
CGTTGAACGGCTCAGCGAGGAAGAAGCAGTAACCCGCCGTGAAGAGAAGTTCCCATATTCCGGCGACAAGCTCTCGCTAA
ACTTTCAGGACATTGAAGTTCGCTCGGTGCTTCAGCTGATTGCTGACTTTACCGGTTTGAATCTGGTGGCCAGCGATACC
GTTGGTGGAAGCATCACGCTGCGCTTGCAGAATGTGCCTTGGGATCAGGCGCTGGATCTCATTTTGAAAACCAAAGGCTT
GGATAAGCGCCAGATTGGCAATGTATTACTGGTAGCGCCCGCCGATGAAATCGCCGCTCGTGAACGGCTGGAACTGGAAA
CAACCAAGCAGATCGCCGAGCTTGCCCCAGTGCGTTTGGACATCATTCAGGTCAATTACGCAAAAGCGGCCGAAGTAGTA
GCGTTGGTGCAGGCCGACCAAGAGCTGATTTCAAGCCGCGGTTTCATTTCCTCAGACGACCGAACCAACACCATCAGCGT
GCGCGAAACCATTGAAAAACTGGACGAGATCCGTCGCCTGGTGTCCACCTGGGACGTCCCCGTGCGTCAGGTTTCCATTG
AAGCCCGGATTGTGCGCGCACAAACCAACGTGGCCGAGAGCCTGGGTGTGAGCTGGGGCGGAGCAGCTTACAGCGTGAGC
GGCAACAACGTAATTTCCGTAGGTGGCTCACAATCTGCAGTTGAGGAGGCACGCAACGCTGCGACCGGCGGATCTGGAAC
GATTACTTTCCCTGGGGCGTTAGCGGTAGATCTGGGCGTTAGCGGCGACGGGGCCTCTTCTTTTGCGATTGGTTTTGGCA
GCGATGACTTCCTGGTAGACCTTGAACTGTCTGCGTTGGAAAGTGATGGTAAAGCGGAAATCGTGTCTCAGCCCAGAGTT
GTTACAGCGGACCGCCAGACGGCGTCTATCAAATCGGGTCAGGAAATCCCGTATCAGGAAGCCAGCAGCAGCGGCGCCAC
CAGCACTTCCTTTAAAGAAGCGGTGTTGTCATTGGAGGTAACGCCGCAGATTACACCGGATGACAAAATCATCATGGACC
TTGAGGTGACTCAGGACTCCCGTGGCGAAGACACCCCCAGCGGTCCGGCCATCAACACCAACTCGGTGACAACTCAGGTA
CTGGTCGCCAACGGTGAAACCATTGTTTTGGGCGGTATTTTCGAATCGACCAACACCGAAACCACCACCAAGACACCGTT
CTTGGGCGATATTCCCTACCTGGGCAGGTTGTTCAAGCGCACTCAGACTACCGAGCTGCGTAGCGAACTGCTGATTTTCA
TCACCCCGAAAATCATCAAAAACGACCTGGTTCGTTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(388-450)

Bacterial type II and III secretion system protein (Secretin)

(545-700)

Secretin and TonB N terminus short domain (STN)

(314-360)

AMIN domain (AMIN)

(54-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

50.631

100

0.512
  comQ Acinetobacter baylyi ADP1

41.949

100

0.421
  pilQ Acinetobacter baumannii D1279779

41.111

100

0.42
  pilQ Acinetobacter baumannii strain A118

41.111

100

0.42
  pilQ Legionella pneumophila strain ERS1305867

40.653

95.603

0.389
  pilQ Legionella pneumophila strain Lp02

40.504

95.603

0.387