Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JN531_RS02090 Genome accession   NZ_CP092104
Coordinates   425273..427003 (+) Length   576 a.a.
NCBI ID   WP_366522457.1    Uniprot ID   -
Organism   Flagellatimonas centrodinii strain R2A-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 420273..432003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN531_RS02070 (JN531_002070) mpl 421126..422469 (-) 1344 WP_228347198.1 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase -
  JN531_RS02075 (JN531_002075) - 422471..423049 (-) 579 WP_228347199.1 hypothetical protein -
  JN531_RS02080 (JN531_002080) rnd 423113..424210 (+) 1098 WP_228347200.1 ribonuclease D -
  JN531_RS02085 (JN531_002085) - 424239..425210 (+) 972 WP_228347201.1 MBL fold metallo-hydrolase -
  JN531_RS02090 (JN531_002090) pilB 425273..427003 (+) 1731 WP_366522457.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JN531_RS02095 (JN531_002095) pilC 427065..428282 (+) 1218 WP_228347203.1 type II secretion system F family protein Machinery gene
  JN531_RS02100 (JN531_002100) pilD 428282..429151 (+) 870 WP_275591474.1 A24 family peptidase Machinery gene
  JN531_RS02105 (JN531_002105) coaE 429148..429765 (+) 618 WP_228347204.1 dephospho-CoA kinase -
  JN531_RS02110 (JN531_002110) zapD 429819..430595 (+) 777 WP_228347205.1 cell division protein ZapD -
  JN531_RS02115 (JN531_002115) - 430592..430795 (+) 204 WP_228347206.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62911.89 Da        Isoelectric Point: 5.4323

>NTDB_id=655868 JN531_RS02090 WP_366522457.1 425273..427003(+) (pilB) [Flagellatimonas centrodinii strain R2A-3]
MSTQTPASGFLGGLARRLAADQLLTEQQARDLQQKALKEQRPFATMVVESKLVSAAALAESASLEFGVPLMDLSAVEMDR
ALIQQLSEKILRKAHALPIYSRGKKMFIAVSDPMNVQALDDIKFATGHATEAVLVEEDKLARALEQALTVLQQADMSVAD
GDLENLEISDEDEHAGSGNDVSRDGADDAPIVRFVNKVMVDAINQGASDIHFEPYEKFYRIRYRKDGELKTIAQPPVSQG
HRLAARIKVMSRMDLAERRVPQDGRIKLNLSKTKAIDFRVSTCPTLFGEKICARILDPSSAQLGIDALGYEQEQKEAYLR
SLQQPQGMILVTGPTGSGKTVSLYTGLNILNTDDINISTAEDPAEINLPGVNQVNVNPKQGLTFAAALKAFLRQDPDVIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNDAPQTIVRLMNMGVPAYNLASTLSLIIAQRLARRLCKHCKRPANIPRPEL
LKQGFTEAQIDSPDFAPHEAVGCDQCDNGYKGRTGIYQVMPFSDAMGRIIMEGGNARDITEQAKTEGIMDLRQSALKKVR
DGQIDLIQANACTVGD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=655868 JN531_RS02090 WP_366522457.1 425273..427003(+) (pilB) [Flagellatimonas centrodinii strain R2A-3]
ATCAGCACGCAGACCCCGGCATCCGGCTTCCTCGGTGGGTTGGCACGGCGCCTCGCCGCCGACCAGCTGTTGACCGAGCA
GCAGGCCCGCGACCTGCAGCAGAAGGCCCTCAAGGAGCAACGCCCCTTCGCCACCATGGTGGTGGAGAGCAAGCTGGTGA
GCGCCGCTGCGCTGGCGGAGTCCGCCAGCCTCGAGTTCGGCGTGCCGCTGATGGACCTGTCGGCGGTGGAAATGGACCGC
GCGCTGATTCAACAGCTCAGCGAAAAAATTCTTCGCAAGGCGCACGCCCTGCCCATCTACTCACGTGGCAAAAAGATGTT
CATCGCGGTCTCCGACCCGATGAACGTCCAGGCCCTGGATGACATCAAGTTCGCTACCGGGCATGCCACCGAGGCGGTGC
TGGTGGAAGAGGACAAGCTGGCACGGGCACTGGAACAGGCGCTGACGGTGCTGCAGCAGGCTGACATGTCGGTGGCCGAC
GGCGACCTCGAGAACCTCGAGATTTCCGACGAGGACGAGCATGCTGGCAGCGGCAACGATGTCTCCCGCGATGGCGCCGA
TGACGCGCCGATCGTGCGCTTCGTCAACAAGGTGATGGTCGACGCCATCAACCAGGGCGCGTCCGATATCCACTTCGAGC
CCTACGAGAAGTTCTATCGCATCCGCTACCGCAAGGACGGCGAGCTCAAGACCATCGCCCAGCCGCCGGTCAGCCAGGGG
CACCGTCTGGCCGCCCGCATCAAGGTGATGAGCCGCATGGACCTCGCCGAACGGCGGGTGCCGCAGGACGGCCGCATCAA
GCTCAACCTGTCGAAGACCAAGGCAATCGACTTTCGTGTCTCGACCTGCCCGACGCTGTTCGGCGAAAAGATCTGCGCCC
GTATCCTTGATCCCTCGTCGGCCCAGCTCGGAATCGATGCTCTGGGCTACGAACAGGAACAGAAGGAAGCCTACCTGCGG
TCGCTGCAGCAGCCCCAGGGGATGATTCTGGTGACCGGGCCGACCGGCTCGGGGAAGACGGTGTCGCTCTACACCGGCCT
CAATATCCTCAACACCGACGACATCAACATCTCGACCGCCGAAGACCCGGCGGAAATCAACCTGCCGGGCGTCAATCAGG
TCAACGTCAATCCGAAACAGGGGCTGACCTTCGCCGCGGCCCTGAAGGCCTTTCTGCGACAGGACCCTGACGTCATCATG
GTGGGGGAAATTCGCGACCTGGAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGTCACCTGGTGTTGTCGACCCT
GCACACCAATGACGCGCCGCAAACCATCGTCCGCCTGATGAACATGGGGGTGCCGGCCTACAACCTGGCATCGACACTGT
CGCTGATCATCGCCCAGCGGCTGGCACGACGCCTGTGCAAACACTGCAAGCGCCCGGCCAATATCCCGCGCCCGGAGCTG
CTGAAACAGGGCTTCACCGAGGCCCAGATCGACAGCCCGGATTTCGCCCCGCATGAGGCGGTTGGCTGCGACCAGTGCGA
CAACGGCTACAAGGGCCGGACCGGGATCTACCAGGTGATGCCGTTTTCCGACGCCATGGGCCGCATCATCATGGAAGGCG
GCAACGCCCGCGACATCACCGAGCAGGCCAAGACCGAAGGGATCATGGATCTGCGCCAGTCGGCCCTGAAAAAGGTCCGT
GACGGCCAGATCGACCTGATTCAGGCCAATGCCTGCACCGTCGGCGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-147)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.401

98.611

0.536
  pilB Acinetobacter baylyi ADP1

53.484

99.653

0.533
  pilB Legionella pneumophila strain ERS1305867

51.579

98.958

0.51
  pilB Vibrio cholerae strain A1552

51.799

96.528

0.5
  pilF Neisseria gonorrhoeae MS11

49.298

98.958

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

49.545

95.313

0.472
  pilB Vibrio campbellii strain DS40M4

48.195

96.181

0.464