Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L8E65_RS24425 Genome accession   NZ_CP091880
Coordinates   5246153..5247853 (+) Length   566 a.a.
NCBI ID   WP_003107297.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain US449     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5241153..5252853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L8E65_RS24405 (L8E65_24390) - 5241867..5244122 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  L8E65_RS24410 (L8E65_24395) nadC 5244408..5245256 (+) 849 WP_047688821.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L8E65_RS24420 (L8E65_24405) - 5245459..5245923 (-) 465 WP_031630343.1 pilin -
  L8E65_RS24425 (L8E65_24410) pilB 5246153..5247853 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L8E65_RS24430 (L8E65_24415) pilC 5247857..5249074 (+) 1218 WP_034014663.1 type II secretion system F family protein Machinery gene
  L8E65_RS24435 (L8E65_24420) pilD 5249075..5249947 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  L8E65_RS24440 (L8E65_24425) coaE 5249944..5250555 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  L8E65_RS24445 (L8E65_24430) yacG 5250552..5250752 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  L8E65_RS24450 (L8E65_24435) - 5250789..5250998 (-) 210 WP_003094660.1 hypothetical protein -
  L8E65_RS24455 (L8E65_24440) - 5251104..5251793 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  L8E65_RS24460 (L8E65_24445) - 5251790..5252260 (-) 471 WP_003094664.1 hypothetical protein -
  L8E65_RS24465 (L8E65_24450) - 5252257..5252682 (-) 426 WP_003094666.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62312.21 Da        Isoelectric Point: 5.4470

>NTDB_id=654196 L8E65_RS24425 WP_003107297.1 5246153..5247853(+) (pilB) [Pseudomonas aeruginosa strain US449]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=654196 L8E65_RS24425 WP_003107297.1 5246153..5247853(+) (pilB) [Pseudomonas aeruginosa strain US449]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCATCAGGCGGTCACCGATATCCAGTTCAGCACCGGCCTCACCACCGAAGCCCTGCTGGTAGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTTTACGAGGGCGCCATGGACGCCCTCGGCGACCTCGGCGATATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGACGCCCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTATCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTATCGAAGAACAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCTTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCACTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGTCTGAATATCCTCAATACCACCGA
CATCAACATCTCCACCGCTGAAGACCCTGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCCGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.544

100

0.58
  pilB Acinetobacter baylyi ADP1

56.79

100

0.569
  pilB Legionella pneumophila strain ERS1305867

54.497

100

0.546
  pilB Vibrio cholerae strain A1552

51.521

98.763

0.509
  pilF Neisseria gonorrhoeae MS11

50.177

99.647

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

52.158

94.17

0.491
  pilB Vibrio campbellii strain DS40M4

51.22

94.17

0.482
  pilB/pilB1 Synechocystis sp. PCC 6803

37.763

100

0.412
  pilF Thermus thermophilus HB27

38.664

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.727

97.173

0.376