Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LVJ80_RS04655 Genome accession   NZ_CP091519
Coordinates   886075..887760 (-) Length   561 a.a.
NCBI ID   WP_034294925.1    Uniprot ID   -
Organism   Alysiella crassa strain S6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 881075..892760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ80_RS04630 (LVJ80_05320) hfq 881917..882231 (+) 315 WP_034295644.1 RNA chaperone Hfq -
  LVJ80_RS04635 (LVJ80_05315) zapD 882403..883152 (-) 750 WP_034294937.1 cell division protein ZapD -
  LVJ80_RS04640 (LVJ80_05310) coaE 883154..883774 (-) 621 WP_034294935.1 dephospho-CoA kinase -
  LVJ80_RS04645 (LVJ80_05305) pilD 883811..884713 (-) 903 WP_051968598.1 A24 family peptidase Machinery gene
  LVJ80_RS04650 (LVJ80_05300) pilG 884715..885986 (-) 1272 WP_034294928.1 type II secretion system F family protein Machinery gene
  LVJ80_RS04655 (LVJ80_05295) pilF 886075..887760 (-) 1686 WP_034294925.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVJ80_RS04660 (LVJ80_05290) - 887898..888341 (-) 444 WP_084693589.1 DUF4149 domain-containing protein -
  LVJ80_RS04665 (LVJ80_05285) greA 888456..888932 (-) 477 WP_034294922.1 transcription elongation factor GreA -
  LVJ80_RS04670 (LVJ80_05280) - 889328..889831 (-) 504 WP_034294920.1 hypothetical protein -
  LVJ80_RS04675 (LVJ80_15245) - 889927..890061 (+) 135 WP_280517559.1 hypothetical protein -
  LVJ80_RS04680 (LVJ80_05275) - 890109..891629 (+) 1521 WP_051968597.1 OmpA family protein -
  LVJ80_RS04685 (LVJ80_05270) nth 891705..892340 (+) 636 WP_034294918.1 endonuclease III -
  LVJ80_RS04690 (LVJ80_15250) - 892364..892495 (+) 132 WP_280517558.1 hypothetical protein -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62570.38 Da        Isoelectric Point: 5.3382

>NTDB_id=652007 LVJ80_RS04655 WP_034294925.1 886075..887760(-) (pilF) [Alysiella crassa strain S6]
MSIGLLRVLIQQKLVPQDKVEKYQKTIESGKKDIIAQIINDKVISAEDLSALMSRLFNYPQFDLKSFPTKRIQMDILDEE
QIQIQRCIPLFKRGRKIFVAVSDPTILQNVQKAVFNSGLSVELIIVRNDHLDNLLEELQQSSASLIKEMGMDATLQESQA
QTMIVGGEEEEDGPIARFIQKILFDAVNTGASDIHFEFYEFMARVRYRTDGVLREIVQPPLNLRSQLASRIKVMAKLDIS
EKRVPQDGRIQLQFSKNTKAIDFRVNTMPCLFGEKVVMRILNSDASFLNINQLGFEDFQKELILDAISRPYGMVLVTGPT
GSGKTVSLYTFLNILNTDDVNISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDIIMIGEIRDLGTADIAIK
AAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASAVNLIMAQRLARRLCSSCKAPMERPPEAVLKEAGFTDEDLAKDW
TMYRAVGCDNCKGKGYKGRAGLYEVMPMTEKMRAVIMKGGTEVDIANIAYEEGLCDLRRSGLLKVMQGVTTLEEVVATTN
E

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=652007 LVJ80_RS04655 WP_034294925.1 886075..887760(-) (pilF) [Alysiella crassa strain S6]
ATGAGTATCGGTTTACTACGCGTTTTAATACAGCAAAAACTTGTTCCGCAAGACAAGGTGGAAAAATATCAGAAAACGAT
TGAGAGCGGTAAAAAAGACATTATTGCTCAAATCATTAATGATAAAGTAATTTCAGCGGAAGATTTATCGGCGTTGATGT
CGCGTTTGTTTAACTATCCACAATTTGATTTGAAATCGTTTCCGACCAAACGCATTCAAATGGATATTTTGGACGAAGAA
CAAATTCAAATTCAACGTTGCATTCCCTTGTTTAAACGTGGGCGCAAAATATTTGTTGCCGTGTCCGACCCGACCATTTT
GCAAAATGTGCAAAAGGCGGTGTTTAATTCGGGTTTATCCGTTGAGCTGATTATTGTCCGTAATGATCATTTGGATAATT
TATTGGAAGAATTGCAACAAAGTAGCGCATCCCTGATTAAAGAAATGGGCATGGACGCGACTTTGCAAGAATCACAAGCG
CAAACCATGATTGTGGGCGGCGAAGAAGAGGAAGATGGTCCAATCGCCCGCTTTATTCAGAAAATTTTGTTTGACGCGGT
GAATACAGGTGCTTCGGATATTCACTTTGAGTTTTACGAATTTATGGCGCGGGTGCGTTACCGTACCGATGGTGTGTTGC
GTGAAATTGTGCAGCCGCCGTTGAATTTGCGTAGCCAATTGGCTTCGCGGATTAAGGTGATGGCGAAATTGGATATTTCT
GAAAAACGCGTACCACAGGACGGACGTATTCAGTTGCAATTTTCCAAAAATACCAAAGCAATTGACTTTCGTGTGAACAC
CATGCCTTGTTTGTTTGGCGAAAAAGTCGTAATGCGTATTTTGAATTCGGACGCATCTTTCCTGAATATCAATCAGTTGG
GTTTTGAAGATTTCCAAAAAGAATTGATTTTGGATGCGATTAGCCGTCCGTATGGCATGGTGCTGGTAACGGGGCCAACA
GGTTCGGGTAAAACCGTGTCGCTGTACACCTTCTTGAATATTTTGAACACGGACGATGTGAACATTTCCACCGCCGAAGA
CCCTGCAGAGATTAACTTACCAGGGATTAACCAAGTAAACGTGAACGATAAGCAAGGCTTAACTTTTGAGGCAGCCTTAA
AAGCGTTCTTGCGTCAAGACCCTGATATCATCATGATTGGTGAGATTCGCGACTTGGGTACGGCGGATATTGCGATTAAA
GCGGCGCAAACGGGTCATATGGTGTTCTCCACCTTGCACACCAATAATGCGCCAGCGACCTTGTCGCGTATGTTGAATAT
GGGTGTGGCACCGTTTAATATTGCTTCGGCGGTGAACTTGATTATGGCGCAACGTCTGGCGCGCCGTTTGTGTTCAAGCT
GTAAAGCCCCAATGGAACGTCCGCCCGAAGCCGTGTTGAAAGAAGCAGGTTTCACAGACGAAGATTTGGCAAAAGATTGG
ACGATGTATCGCGCTGTGGGATGTGATAATTGCAAGGGCAAAGGCTATAAAGGTCGTGCGGGTTTGTATGAAGTAATGCC
GATGACGGAAAAAATGCGCGCCGTGATTATGAAAGGCGGTACGGAAGTGGACATTGCCAATATTGCTTATGAAGAAGGCT
TGTGCGATTTGCGCCGTTCTGGCTTGCTCAAAGTCATGCAGGGCGTAACCACATTGGAAGAAGTGGTGGCAACCACCAAC
GAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-144)

Type II/IV secretion system protein (T2SSE)

(181-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

66.31

100

0.663
  pilB Acinetobacter baumannii D1279779

52.491

100

0.526
  pilB Acinetobacter baylyi ADP1

51.779

100

0.519
  pilB Legionella pneumophila strain ERS1305867

47.857

99.822

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

43.717

100

0.44
  pilB Vibrio cholerae strain A1552

43.95

100

0.44
  pilB Vibrio campbellii strain DS40M4

45.265

94.118

0.426