Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LVJ80_RS00765 Genome accession   NZ_CP091519
Coordinates   125761..126948 (-) Length   395 a.a.
NCBI ID   WP_034295243.1    Uniprot ID   A0A376BUD8
Organism   Alysiella crassa strain S6     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 120761..131948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ80_RS00735 (LVJ80_15050) - 121208..121714 (-) 507 WP_172540004.1 ISAs1 family transposase -
  LVJ80_RS00740 (LVJ80_15055) - 121734..122297 (-) 564 WP_115723569.1 ISAs1 family transposase -
  LVJ80_RS00745 (LVJ80_09575) - 122477..123571 (-) 1095 WP_051968614.1 tetratricopeptide repeat protein -
  LVJ80_RS00750 (LVJ80_09570) - 123703..124404 (-) 702 WP_034295248.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  LVJ80_RS00755 (LVJ80_09565) - 124653..125132 (-) 480 WP_034295247.1 S-ribosylhomocysteine lyase -
  LVJ80_RS00760 (LVJ80_09560) - 125177..125521 (-) 345 WP_034295245.1 DUF2069 domain-containing protein -
  LVJ80_RS00765 (LVJ80_09555) pilU 125761..126948 (-) 1188 WP_034295243.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LVJ80_RS00770 (LVJ80_09550) acnB 127235..129823 (+) 2589 WP_034295241.1 bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase -
  LVJ80_RS00775 (LVJ80_09545) - 129885..130289 (-) 405 WP_034295239.1 helix-turn-helix domain-containing protein -
  LVJ80_RS00780 (LVJ80_09540) - 130414..131001 (+) 588 WP_034295237.1 transferrin-binding protein-like solute binding protein -

Sequence

Protein


Download         Length: 395 a.a.        Molecular weight: 43956.45 Da        Isoelectric Point: 6.1357

>NTDB_id=651999 LVJ80_RS00765 WP_034295243.1 125761..126948(-) (pilU) [Alysiella crassa strain S6]
MSTVHIPPVSEEMKKFIHGLLTHMVKNKGSDLFITAGYPPAMKLDGKLTKITDKPLTADHTQQIARSIMDDKQAEEFFTT
NECNFAISLAGVSRFRINAMVQRGATALVCRVITSDIPKFDNMNLPPVLKQVVMEKRGLVIFVGGTGSGKSTSLAAMIDY
RNANSYGHIITIEDPIEFVHPHQNCIITQREVGVDTADWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCMA
TLHANNSNQALDRIINFFPEERRTQLLNDLSLNLKGFISQRLIPKKEGKGRCAAVEILLNSPLISELILHGDVHGVKEIM
ARSRDIGMQTFDQSLFDLYEEDLISYDEALKNADSVNDLRLQIQLNSKKNKTAGSDGILDGLALTDYEAPEEVKK

Nucleotide


Download         Length: 1188 bp        

>NTDB_id=651999 LVJ80_RS00765 WP_034295243.1 125761..126948(-) (pilU) [Alysiella crassa strain S6]
ATGTCCACAGTCCATATTCCGCCAGTTAGCGAAGAGATGAAAAAATTTATTCATGGCTTACTGACGCACATGGTAAAAAA
CAAAGGTTCGGATTTATTCATCACGGCTGGTTATCCGCCAGCGATGAAATTGGACGGTAAATTAACCAAAATTACCGACA
AACCCCTCACCGCCGACCACACCCAACAAATCGCCCGCTCCATTATGGACGACAAACAAGCGGAAGAATTTTTCACCACC
AATGAATGTAATTTTGCGATTAGTTTGGCGGGGGTGTCGCGTTTTCGTATCAATGCGATGGTGCAACGTGGTGCAACTGC
GCTGGTGTGCCGTGTGATTACCAGCGATATTCCGAAATTTGACAATATGAATTTGCCGCCTGTTTTGAAACAAGTGGTGA
TGGAAAAACGTGGTCTCGTGATTTTTGTGGGCGGCACGGGTTCGGGTAAATCCACGTCTTTGGCGGCGATGATTGATTAC
CGCAACGCCAACAGTTATGGGCATATTATTACCATTGAAGATCCGATTGAATTTGTTCACCCGCACCAAAACTGCATTAT
CACGCAACGCGAAGTGGGTGTGGACACCGCCGACTGGTTTGCCGCGCTGAAAAATACGCTGCGCCAAGCCCCAGATGTGA
TTTTGATTGGCGAAATCCGCGACCGCGAAACGATGGACTACGCTTTGGCGTTTGCCGAAACAGGGCATTTGTGTATGGCA
ACGCTGCACGCGAATAACTCCAACCAAGCCCTTGACCGCATTATTAACTTTTTCCCTGAAGAACGCCGCACGCAATTATT
AAACGATTTATCTTTGAATTTAAAAGGATTTATTTCGCAGCGTTTGATTCCGAAAAAAGAAGGCAAAGGGCGTTGTGCGG
CGGTGGAAATTTTGCTTAATTCGCCCTTGATTTCGGAACTGATTTTGCATGGCGATGTGCATGGCGTAAAAGAAATCATG
GCGCGTTCACGCGATATTGGTATGCAGACTTTTGACCAATCGTTGTTTGATTTGTATGAAGAAGATTTGATTAGCTACGA
TGAAGCCTTGAAAAATGCGGATTCGGTCAATGACTTGCGTTTGCAAATTCAGTTGAACAGTAAGAAAAACAAAACAGCAG
GTTCAGATGGCATTTTGGACGGTTTGGCTTTGACGGATTACGAAGCACCTGAAGAAGTGAAAAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A376BUD8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

59.207

89.367

0.529
  pilU Pseudomonas stutzeri DSM 10701

59.259

88.861

0.527
  pilU Vibrio cholerae strain A1552

53.297

92.152

0.491
  pilT Legionella pneumophila strain Lp02

43.243

84.304

0.365
  pilT Legionella pneumophila strain ERS1305867

43.243

84.304

0.365
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.072

84.051

0.362