Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LVJ86_RS00580 Genome accession   NZ_CP091510
Coordinates   132223..133905 (-) Length   560 a.a.
NCBI ID   WP_047760859.1    Uniprot ID   A0A0J1C448
Organism   Neisseria arctica strain KH1503     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 127223..138905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ86_RS00545 (LVJ86_00545) - 127295..127750 (-) 456 WP_047760853.1 pilin -
  LVJ86_RS00555 (LVJ86_00555) yacG 128530..128712 (-) 183 WP_047760854.1 DNA gyrase inhibitor YacG -
  LVJ86_RS00560 (LVJ86_00560) zapD 128728..129492 (-) 765 WP_047760855.1 cell division protein ZapD -
  LVJ86_RS00565 (LVJ86_00565) coaE 129489..130109 (-) 621 WP_047760856.1 dephospho-CoA kinase -
  LVJ86_RS00570 (LVJ86_00570) pilD 130106..130969 (-) 864 WP_047760857.1 A24 family peptidase Machinery gene
  LVJ86_RS00575 (LVJ86_00575) pilG 130971..132212 (-) 1242 WP_047760858.1 type II secretion system F family protein Machinery gene
  LVJ86_RS00580 (LVJ86_00580) pilF 132223..133905 (-) 1683 WP_047760859.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVJ86_RS00585 (LVJ86_00585) - 134134..135201 (-) 1068 WP_082131251.1 RnfABCDGE type electron transport complex subunit B -
  LVJ86_RS00590 (LVJ86_00590) - 135141..136169 (-) 1029 WP_047760887.1 lysozyme inhibitor LprI family protein -
  LVJ86_RS00595 (LVJ86_00595) - 136424..137665 (-) 1242 WP_047760861.1 aromatic amino acid transporter -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 62365.88 Da        Isoelectric Point: 6.0856

>NTDB_id=651848 LVJ86_RS00580 WP_047760859.1 132223..133905(-) (pilF) [Neisseria arctica strain KH1503]
MSVGLLRVLVQSQIVTGDKIERYQKALDDYKDIIPMLFDEHIITPQALGELLARIFSYPLLDLRYFPRTNILTDVLTEEQ
MLQNRCIPIFRRGRKVFLAVSDPTQIQNFQKAAFTSGTSIDLVIVRDDQLSTLLEWLGQRSTAILNEISKEQSDSAQQGG
MLYVDNEEAEDGPIARFIHKTLSDALSAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISE
KRVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAGSLNIDQLGFEPFQKELLLDAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTENVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKAFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKAPAERPPLPALKKAGFSDEDIAKDWT
LYRPVGCDSCRGKGFKGRVGVYEVMPVTEEMQRVIMENGTEIDIQNMAYKQGMVDLRHAGLLKVMQGLTSLEEVLANTND

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=651848 LVJ86_RS00580 WP_047760859.1 132223..133905(-) (pilF) [Neisseria arctica strain KH1503]
ATGAGTGTTGGGTTATTAAGAGTACTGGTACAAAGCCAGATCGTGACCGGCGATAAAATCGAGCGCTATCAAAAAGCATT
GGATGATTACAAAGACATCATCCCCATGCTTTTCGATGAACACATCATCACACCCCAAGCACTGGGTGAATTATTAGCCC
GCATTTTTAGCTATCCACTGTTGGATCTGCGCTACTTTCCCCGTACCAATATCCTTACCGATGTGTTGACGGAAGAACAA
ATGCTGCAGAACCGCTGCATACCGATTTTCCGCCGCGGTCGGAAAGTATTTTTAGCCGTCTCCGACCCCACCCAAATTCA
AAACTTCCAAAAGGCCGCCTTCACCTCAGGCACATCAATAGATTTGGTCATCGTCCGTGACGATCAACTCAGCACCCTGC
TCGAATGGTTAGGACAAAGGTCAACAGCCATCCTTAATGAAATCAGCAAAGAACAATCGGATTCGGCCCAGCAGGGCGGT
ATGCTGTATGTGGATAACGAAGAAGCCGAAGATGGTCCGATTGCCCGGTTCATCCACAAAACCTTATCCGATGCCCTTAG
TGCCGGCGCTTCAGATATCCATTTTGAATTTTACGAACAAATGGCTCGCGTACGTTTCCGCGTAGACGGGCAGTTGCGTG
AAGTAGTCCAACCTCCCGTAGCCGTACGCGGACAATTGGCTTCACGCATCAAAGTAATGGCAAGACTCGATATTTCCGAA
AAGCGTGTACCGCAAGACGGTAGAATTCAAATCGCATTTCACAAACATGGCCGCCCGATTGACTTCCGTGTCAGCACACT
GCCCACCTTGTTTGGCGAAAAAGTAGTGATGCGGATTCTGAATTCAGATGCCGGCTCCCTAAATATCGACCAACTCGGTT
TCGAGCCTTTCCAAAAAGAATTACTGCTCGATGCCATTCACCGCCCCTACGGCATGGTTTTGGTGACCGGTCCGACAGGC
TCGGGCAAAACCGTCTCACTGTATACCTGTCTGAATATTCTGAATACCGAAAATGTAAACATTGCCACGGCGGAAGACCC
GGCAGAAATTAATTTACCCGGCATTAACCAAGTCAACGTAAATGACAAACAAGGTCTTACCTTTGCCGCTGCCCTGAAAG
CATTCTTGCGTCAAGATCCGGACATTATCATGGTAGGGGAGATCCGCGACCTTGAAACGGCCGACATCGCCATTAAAGCC
GCACAAACCGGTCACATGGTATTCTCAACCCTACACACCAATAATGCCCCTGCCACTTTGTCCCGCATGCTCAATATGGG
CGTTGCCCCATTTAATATCGCCAGTTCGGTCAGCCTGATTATGGCACAGCGCTTGCTACGCCGTTTATGTCCAAACTGTA
AAGCACCTGCCGAGCGTCCACCGCTGCCGGCACTGAAAAAAGCAGGTTTTTCAGATGAGGATATTGCAAAAGATTGGACG
CTATACCGCCCCGTCGGTTGTGACAGCTGCCGGGGTAAAGGATTCAAAGGACGTGTAGGCGTCTATGAAGTTATGCCGGT
CACCGAAGAAATGCAACGCGTGATCATGGAAAACGGCACCGAAATTGATATCCAAAATATGGCTTATAAACAAGGCATGG
TAGACTTACGCCATGCAGGTTTATTAAAAGTTATGCAAGGGCTGACCTCACTAGAAGAAGTATTGGCAAATACCAACGAT
TAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(180-448)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J1C448

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

75.714

100

0.757
  pilB Acinetobacter baumannii D1279779

51.607

100

0.516
  pilB Acinetobacter baylyi ADP1

50.719

99.286

0.504
  pilB Legionella pneumophila strain ERS1305867

46.263

100

0.464
  pilB Vibrio cholerae strain A1552

44.938

100

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

46.3

94.107

0.436
  pilB Vibrio campbellii strain DS40M4

43.085

100

0.434
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.499

92.679

0.366