Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LZ558_RS03305 Genome accession   NZ_CP091424
Coordinates   740460..741626 (-) Length   388 a.a.
NCBI ID   WP_268119405.1    Uniprot ID   -
Organism   Methylobacter sp. YRD-M1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 735460..746626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZ558_RS03285 (LZ558_03260) - 736076..736828 (-) 753 WP_268119399.1 lysophospholipid acyltransferase family protein -
  LZ558_RS03290 (LZ558_03265) gmhB 736794..737333 (-) 540 WP_268119400.1 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase -
  LZ558_RS03295 (LZ558_03270) glyS 737342..739414 (-) 2073 WP_268119401.1 glycine--tRNA ligase subunit beta -
  LZ558_RS03300 (LZ558_03275) glyQ 739414..740331 (-) 918 WP_268119403.1 glycine--tRNA ligase subunit alpha -
  LZ558_RS03305 (LZ558_03280) pilU 740460..741626 (-) 1167 WP_268119405.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LZ558_RS03310 (LZ558_03285) - 741750..742796 (+) 1047 WP_268119406.1 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase -
  LZ558_RS03315 (LZ558_03290) - 742793..743020 (+) 228 WP_268119407.1 sulfurtransferase TusA family protein -
  LZ558_RS03320 (LZ558_03295) - 743020..744165 (+) 1146 WP_268119408.1 cation diffusion facilitator family transporter -
  LZ558_RS03325 (LZ558_03300) argJ 744178..745398 (+) 1221 WP_268119409.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  LZ558_RS03330 (LZ558_03305) - 745438..746367 (+) 930 WP_268119410.1 Nudix family hydrolase -
  LZ558_RS03335 (LZ558_03310) yacG 746369..746569 (-) 201 WP_268119411.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43026.14 Da        Isoelectric Point: 4.9688

>NTDB_id=651084 LZ558_RS03305 WP_268119405.1 740460..741626(-) (pilU) [Methylobacter sp. YRD-M1]
MEFKDYLKILVLKDGSDLYLTVDAPPAAKFEGSLKPLENIRLTKERIKEIAYSLMDEEQQKAFEQVPEMNLALSEPGIGR
FRVNIFKQRNSLALVIRNIKVDIPNADKLGLPPVLKQSIMEKRGLILFVGGTGSGKSTSLAALIDYRNSHASGHIITIED
PIEYIHPHKKSLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLLDLSLNLKAFVSQRLVPTVDGKRTAAIEILLGTQLARDLIQKGEIHAIKEAMEKSEEIGMQTFDSHL
LRLFKEGTISLEEALNNSDSPNNLKLKINLSEGLGSVSPAQTSDAAGSLSGFALEAIDKEEEEEEPAE

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=651084 LZ558_RS03305 WP_268119405.1 740460..741626(-) (pilU) [Methylobacter sp. YRD-M1]
ATGGAATTCAAAGACTACCTTAAAATTCTGGTTCTCAAAGACGGCTCCGACCTTTACTTGACCGTGGACGCGCCGCCAGC
CGCCAAATTTGAAGGCTCGTTGAAGCCTTTGGAAAACATCAGGCTGACCAAGGAGCGCATCAAGGAAATCGCCTATAGCC
TGATGGATGAAGAGCAGCAAAAGGCTTTCGAACAGGTGCCTGAGATGAATCTGGCCCTTTCCGAACCCGGCATCGGCCGC
TTCCGCGTCAATATTTTCAAGCAGCGCAACAGCCTGGCTCTAGTTATCCGGAACATCAAAGTCGACATCCCGAATGCCGA
CAAACTGGGGCTGCCGCCCGTGCTGAAACAATCCATCATGGAAAAGCGCGGCCTGATCCTGTTCGTCGGCGGCACCGGCT
CCGGCAAGTCGACTTCACTGGCGGCCCTGATCGATTACCGCAACAGCCATGCATCGGGCCATATCATCACCATCGAAGAC
CCGATCGAGTACATTCATCCGCACAAAAAATCACTGGTCAACCAGCGCGAAGTCGGCGTTGATACGCTGAGCTACGAAGA
TGCGTTGAAAAACACGCTGCGCCAGGCGCCTGACGTCATTCTGATCGGCGAGATCCGCAGCCAGGAAACCATGGAACACG
CGCTGGCCTTCGCCGAAACCGGCCATCTATGCCTGTCCACACTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAATTTCTTCCCCGAAGAACGCCGCAATCAATTGCTGCTGGATTTGTCGCTGAATTTGAAGGCGTTTGTCTCGCAACG
GCTGGTGCCTACCGTCGACGGCAAGCGCACGGCCGCGATAGAAATCTTATTGGGTACGCAACTGGCCCGCGACCTGATAC
AGAAAGGCGAAATACATGCCATCAAGGAAGCCATGGAGAAATCGGAAGAAATCGGCATGCAGACGTTTGACAGCCATTTG
CTGAGGCTCTTCAAAGAGGGCACGATTTCGCTTGAAGAAGCGCTGAACAATTCCGACTCGCCGAATAACCTCAAGCTGAA
AATCAATCTGTCTGAAGGTCTAGGCTCGGTTTCGCCCGCTCAGACGTCCGATGCGGCCGGCAGCTTGTCAGGCTTTGCGC
TGGAGGCCATCGACAAGGAAGAAGAGGAAGAAGAACCGGCGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.065

94.845

0.541
  pilU Acinetobacter baylyi ADP1

53.39

91.237

0.487
  pilU Vibrio cholerae strain A1552

50.282

91.237

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.209

93.814

0.387
  pilT Legionella pneumophila strain Lp02

41.52

88.144

0.366
  pilT Legionella pneumophila strain ERS1305867

41.52

88.144

0.366