Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   H020_RS0110385 Genome accession   NZ_AKVY01000001
Coordinates   1954605..1955621 (-) Length   338 a.a.
NCBI ID   WP_074196785.1    Uniprot ID   -
Organism   Streptococcus pneumoniae TIGR4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1949605..1960621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H020_RS14575 - 1949682..1949813 (-) 132 WP_000768904.1 hypothetical protein -
  H020_RS0110340 - 1949814..1951004 (-) 1191 WP_000167757.1 acetate kinase -
  H020_RS0110345 comYH 1951055..1952008 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  H020_RS13165 - 1952069..1952663 (-) 595 Protein_1937 class I SAM-dependent methyltransferase -
  H020_RS0110360 comGG/cglG 1952800..1953213 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  H020_RS0110365 comGF/cglF 1953191..1953652 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  H020_RS0110370 comGE/cglE 1953615..1953917 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  H020_RS0110375 comGD/cglD 1953880..1954284 (-) 405 WP_000588028.1 competence type IV pilus minor pilin ComGD Machinery gene
  H020_RS0110380 comGC/cglC 1954277..1954603 (-) 327 WP_000738627.1 comG operon protein ComGC Machinery gene
  H020_RS0110385 comGB/cglB 1954605..1955621 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  H020_RS0110390 comGA/cglA/cilD 1955569..1956510 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  H020_RS0110395 - 1956586..1956951 (-) 366 WP_000286415.1 DUF1033 family protein -
  H020_RS0110400 - 1957102..1958160 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  H020_RS0110405 nagA 1958323..1959474 (-) 1152 WP_001134456.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38392.40 Da        Isoelectric Point: 9.4802

>NTDB_id=64967 H020_RS0110385 WP_074196785.1 1954605..1955621(-) (comGB/cglB) [Streptococcus pneumoniae TIGR4]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEV
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=64967 H020_RS0110385 WP_074196785.1 1954605..1955621(-) (comGB/cglB) [Streptococcus pneumoniae TIGR4]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAGTC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

100

100

1
  comGB/cglB Streptococcus pneumoniae D39

100

100

1
  comGB/cglB Streptococcus pneumoniae R6

100

100

1
  comGB/cglB Streptococcus pneumoniae TIGR4

100

100

1
  comGB/cglB Streptococcus mitis SK321

95.266

100

0.953
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.547

94.083

0.541
  comYB Streptococcus mutans UA159

57.547

94.083

0.541
  comGB Lactococcus lactis subsp. cremoris KW2

51.198

98.817

0.506

Multiple sequence alignment