Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IAI_RS0128895 Genome accession   NZ_AFXJ01000001
Coordinates   6132488..6134632 (-) Length   714 a.a.
NCBI ID   WP_019726987.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa 19BR     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6127488..6139632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IAI_RS0128880 - 6129146..6130795 (-) 1650 WP_019726984.1 AAA family ATPase -
  IAI_RS0128885 aroB 6130802..6131908 (-) 1107 WP_019726985.1 3-dehydroquinate synthase -
  IAI_RS0128890 aroK 6131958..6132476 (-) 519 WP_003095833.1 shikimate kinase AroK -
  IAI_RS0128895 pilQ 6132488..6134632 (-) 2145 WP_019726987.1 type 4a pilus secretin PilQ Machinery gene
  IAI_RS0128900 pilP 6134686..6135210 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  IAI_RS0128905 pilO 6135207..6135830 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  IAI_RS0128910 pilN 6135827..6136423 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  IAI_RS0128915 pilM 6136423..6137487 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77452.98 Da        Isoelectric Point: 5.2285

>NTDB_id=64691 IAI_RS0128895 WP_019726987.1 6132488..6134632(-) (pilQ) [Pseudomonas aeruginosa 19BR]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELRLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASTAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SDGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
SGYGKDGNIGIKDEDGMNCGPIAGSCTFPTTGTSKSPSPFVDLGAKDATSGIGIGFITDNVILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDYQNMLNGVPPINKN
EVNAKILVNDGETIVIGGVFSNEQSKSVEKVPFLGELPYLGRLFRRDTVTDRKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=64691 IAI_RS0128895 WP_019726987.1 6132488..6134632(-) (pilQ) [Pseudomonas aeruginosa 19BR]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCTGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCACTGCCTGGCGACCGGGTCGAGCTAAGGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAACAAACTCGGCACCAAGAATCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGTTGATCATCAACCTGACCACGTT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCACCGCTCCGGTCAAGGCTAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATTCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAACTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACCGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGTGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTCCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGATGGTGGCCAGGAAGGCAAGGAAGGTGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGTATCATCGCCTA
TCAGCCCCAGGAGCGACTGGACGAACTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGG
CACGCATCGTCGAGGCCAACGTCGGTTACGACAAGAGTCTCGGTGTGCGCTGGGGCGGCGCTTATCACAAGGGCAACTGG
AGCGGCTACGGCAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGTAGCTGTAC
CTTCCCGACCACGGGTACCAGCAAGTCGCCGTCGCCGTTCGTCGACCTCGGTGCCAAGGATGCTACCTCCGGGATCGGCA
TCGGCTTCATCACCGACAACGTCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAATGGCGAGATCGTCTCC
CAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTGAAGGGCTCGGAAGTCCCCTACCAGGAGGCCAGTTC
CAGCGGCGCTACCAGCACCTCGTTCAAGGAAGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCGGACAACCGGA
TCATCGTCGAAGTCAAGGTGACCAAGGACGCGCCGGATTACCAGAATATGTTGAACGGCGTACCTCCTATCAACAAGAAC
GAGGTCAACGCCAAGATCCTGGTCAACGACGGCGAGACCATCGTCATCGGCGGCGTGTTCAGTAACGAGCAGAGCAAGTC
GGTGGAGAAGGTACCCTTCCTCGGCGAACTGCCTTACCTCGGGCGGCTGTTCCGGCGAGATACCGTCACCGACCGGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II and III secretion system protein (Secretin)

(549-705)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

99.72

100

0.997
  pilQ Acinetobacter baumannii D1279779

40.839

100

0.409
  pilQ Acinetobacter baumannii strain A118

40.839

100

0.409
  comQ Acinetobacter baylyi ADP1

41.311

98.319

0.406
  pilQ Legionella pneumophila strain Lp02

37.956

95.938

0.364
  pilQ Legionella pneumophila strain ERS1305867

37.81

95.938

0.363

Multiple sequence alignment