Detailed information NTG0646319

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: L0961_RS12720
• Genome accession: NZ_CP090905
• Coordinates: 2591293..2591907 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain Yao
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2586293..2596907

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L0961_RS12690 (L0961_12690)	rpsT	2586593..2586859 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
L0961_RS12695 (L0961_12695)	holA	2586876..2587917 (-)	1042	Protein_2464	DNA polymerase III subunit delta	-
L0961_RS12700 (L0961_12700)	-	2587957..2588111 (-)	155	Protein_2465	hypothetical protein	-
L0961_RS12705 (L0961_12705)	-	2588152..2588286 (+)	135	WP_003152870.1	YqzM family protein	-
L0961_RS12710 (L0961_12710)	comEC	2588305..2590656 (-)	2352	WP_234949402.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
L0961_RS12715 (L0961_12715)	-	2590657..2591226 (-)	570	WP_003152868.1	ComE operon protein 2	-
L0961_RS12720 (L0961_12720)	comEA	2591293..2591907 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
L0961_RS12725 (L0961_12725)	comER	2591966..2592787 (+)	822	WP_014305449.1	late competence protein ComER	-
L0961_RS12730 (L0961_12730)	-	2592856..2593593 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
L0961_RS12735 (L0961_12735)	rsfS	2593590..2593946 (-)	357	WP_003152864.1	ribosome silencing factor	-
L0961_RS12740 (L0961_12740)	yqeK	2593943..2594524 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
L0961_RS12745 (L0961_12745)	-	2594514..2595083 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
L0961_RS12750 (L0961_12750)	yhbY	2595094..2595384 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
L0961_RS12755 (L0961_12755)	aroE	2595378..2596220 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=646319 L0961_RS12720 WP_043867297.1 2591293..2591907(-) (comEA) [Bacillus velezensis strain Yao]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=646319 L0961_RS12720 WP_043867297.1 2591293..2591907(-) (comEA) [Bacillus velezensis strain Yao]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363