Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   L1A14_RS00225 Genome accession   NZ_CP090883
Coordinates   37202..38218 (+) Length   338 a.a.
NCBI ID   WP_074017570.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain LE4448     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 32202..43218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1A14_RS00205 (L1A14_00205) nagA 33349..34500 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  L1A14_RS00210 (L1A14_00210) - 34663..35721 (+) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  L1A14_RS00215 (L1A14_00215) - 35872..36237 (+) 366 WP_000286415.1 DUF1033 family protein -
  L1A14_RS00220 (L1A14_00220) comGA/cglA/cilD 36313..37254 (+) 942 WP_000249552.1 competence type IV pilus ATPase ComGA Machinery gene
  L1A14_RS00225 (L1A14_00225) comGB/cglB 37202..38218 (+) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  L1A14_RS00230 (L1A14_00230) comGC/cglC 38220..38546 (+) 327 WP_000738636.1 comG operon protein ComGC Machinery gene
  L1A14_RS00235 (L1A14_00235) comGD/cglD 38539..38943 (+) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  L1A14_RS00240 (L1A14_00240) comGE/cglE 38906..39208 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  L1A14_RS00245 (L1A14_00245) comGF/cglF 39171..39632 (+) 462 WP_000250542.1 competence type IV pilus minor pilin ComGF Machinery gene
  L1A14_RS00250 (L1A14_00250) comGG/cglG 39610..40023 (+) 414 WP_000265624.1 competence type IV pilus minor pilin ComGG Machinery gene
  L1A14_RS00255 (L1A14_00255) - 40160..40754 (+) 595 Protein_35 methyltransferase domain-containing protein -
  L1A14_RS00260 (L1A14_00260) comYH 40815..41768 (+) 954 WP_000345099.1 class I SAM-dependent methyltransferase Machinery gene
  L1A14_RS00265 (L1A14_00265) - 41819..43009 (+) 1191 WP_000167765.1 acetate kinase -
  L1A14_RS10350 - 43010..43141 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38482.48 Da        Isoelectric Point: 9.6162

>NTDB_id=645653 L1A14_RS00225 WP_074017570.1 37202..38218(+) (comGB/cglB) [Streptococcus pneumoniae strain LE4448]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=645653 L1A14_RS00225 WP_074017570.1 37202..38218(+) (comGB/cglB) [Streptococcus pneumoniae strain LE4448]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae D39

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae R6

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae TIGR4

98.521

100

0.985
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503