Detailed information NTG0645657

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: L1A14_RS00245
• Genome accession: NZ_CP090883
• Coordinates: 39171..39632 (+)
• Length: 153 a.a.
• NCBI ID: WP_000250542.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain LE4448
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 34171..44632

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L1A14_RS00210 (L1A14_00210)	-	34663..35721 (+)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-
L1A14_RS00215 (L1A14_00215)	-	35872..36237 (+)	366	WP_000286415.1	DUF1033 family protein	-
L1A14_RS00220 (L1A14_00220)	comGA/cglA/cilD	36313..37254 (+)	942	WP_000249552.1	competence type IV pilus ATPase ComGA	Machinery gene
L1A14_RS00225 (L1A14_00225)	comGB/cglB	37202..38218 (+)	1017	WP_074017570.1	competence type IV pilus assembly protein ComGB	Machinery gene
L1A14_RS00230 (L1A14_00230)	comGC/cglC	38220..38546 (+)	327	WP_000738636.1	comG operon protein ComGC	Machinery gene
L1A14_RS00235 (L1A14_00235)	comGD/cglD	38539..38943 (+)	405	WP_000588013.1	competence type IV pilus minor pilin ComGD	Machinery gene
L1A14_RS00240 (L1A14_00240)	comGE/cglE	38906..39208 (+)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
L1A14_RS00245 (L1A14_00245)	comGF/cglF	39171..39632 (+)	462	WP_000250542.1	competence type IV pilus minor pilin ComGF	Machinery gene
L1A14_RS00250 (L1A14_00250)	comGG/cglG	39610..40023 (+)	414	WP_000265624.1	competence type IV pilus minor pilin ComGG	Machinery gene
L1A14_RS00255 (L1A14_00255)	-	40160..40754 (+)	595	Protein_35	methyltransferase domain-containing protein	-
L1A14_RS00260 (L1A14_00260)	comYH	40815..41768 (+)	954	WP_000345099.1	class I SAM-dependent methyltransferase	Machinery gene
L1A14_RS00265 (L1A14_00265)	-	41819..43009 (+)	1191	WP_000167765.1	acetate kinase	-
L1A14_RS10350	-	43010..43141 (+)	132	WP_000768904.1	hypothetical protein	-
L1A14_RS00270 (L1A14_00270)	rnpA	43158..43529 (+)	372	WP_000739244.1	ribonuclease P protein component	-
L1A14_RS00275 (L1A14_00275)	-	43504..44328 (+)	825	WP_001809383.1	membrane protein insertase YidC	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17918.53 Da
• Isoelectric Point: 9.3137

>NTDB_id=645657 L1A14_RS00245 WP_000250542.1 39171..39632(+) (comGF/cglF) [Streptococcus pneumoniae strain LE4448]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTNARGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEKS

Nucleotide

• Length: 462 bp

>NTDB_id=645657 L1A14_RS00245 WP_000250542.1 39171..39632(+) (comGF/cglF) [Streptococcus pneumoniae strain LE4448]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTACTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAACTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGATCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACGAATGCTCG
TGGTCGAGGTTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	96.078	100	0.961
comGF/cglF	Streptococcus pneumoniae D39	96.078	100	0.961
comGF/cglF	Streptococcus pneumoniae R6	96.078	100	0.961
comGF/cglF	Streptococcus pneumoniae TIGR4	96.078	100	0.961
comGF/cglF	Streptococcus mitis SK321	92.157	100	0.922
comGF/cglF	Streptococcus mitis NCTC 12261	90.85	100	0.909