Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   SPB_RS05495 Genome accession   NZ_AEUT02000001
Coordinates   1094460..1096193 (-) Length   577 a.a.
NCBI ID   WP_003103640.1    Uniprot ID   F1Z2Q9
Organism   Streptococcus parauberis NCFD 2020     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 1089460..1101193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPB_RS05470 (SPB_1133) - 1090193..1090546 (-) 354 WP_003103672.1 S1 RNA-binding domain-containing protein -
  SPB_RS05475 (SPB_1134) - 1090546..1091946 (-) 1401 WP_003104279.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  SPB_RS05480 (SPB_1135) - 1091979..1092620 (-) 642 WP_003105505.1 response regulator transcription factor -
  SPB_RS05485 (SPB_1136) - 1092613..1093617 (-) 1005 WP_003104853.1 sensor histidine kinase -
  SPB_RS05490 (SPB_1137) liaF 1093614..1094309 (-) 696 WP_037620823.1 cell wall-active antibiotics response protein LiaF -
  SPB_RS05495 (SPB_1138) pknB 1094460..1096193 (-) 1734 WP_003103640.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  SPB_RS05500 (SPB_1139) - 1096193..1096933 (-) 741 WP_003102848.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  SPB_RS05505 (SPB_1140) rsmB 1096971..1098293 (-) 1323 WP_003105444.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  SPB_RS05510 (SPB_1141) fmt 1098283..1099218 (-) 936 WP_003104470.1 methionyl-tRNA formyltransferase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63510.14 Da        Isoelectric Point: 6.2094

>NTDB_id=64546 SPB_RS05495 WP_003103640.1 1094460..1096193(-) (pknB) [Streptococcus parauberis NCFD 2020]
MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVLRTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIG
EEDGQQFLVMEYVDGFDLKRFIQENAPIPNEQVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKDGVVKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAVTIALQHFQKPLPSIIEENKGVP
QALENVVIKATAKRLSDRYATTFDMSRDLMTALSYNRSRERKLVFHDVESTKPLPKVTPVPVTQTKSSVAPQTSGSEIHD
DAVTPQKTKKKRGRLLGTLLKILFALFVVLVAVFAFLVLTKPSTVKIPNVNGTTLSVAKKELTDLGFQVGQIHKMEDAAV
DSGKVIKTDPKAGTERREGARIDLFVSTGQPKVKKKSKDKVLMPDFTNMTYKDVLDMLTNEYGIHPNRIVAYYQDAPNPE
NYSKVSSPKDSQLVMGQYPIVGTYYIISDKEDIELYLSPDSGDNTTVFSSTSTSSSSEEETSTSTETSTSSESTTSSSSS
DATTTDSTTSDTSTTTP

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=64546 SPB_RS05495 WP_003103640.1 1094460..1096193(-) (pknB) [Streptococcus parauberis NCFD 2020]
ATGATACAGATTGGCAAATTATTTGCTGGTCGTTATCGTATCCTGAAGTCTATTGGCCGCGGTGGTATGGCTGATGTATA
TTTAGCCAATGATTTGATATTAGATAATGAAGATGTTGCAATAAAAGTTTTGCGAACCAATTATCAGACGGATCAAGTAG
CAGTAGCTCGTTTCCAACGGGAAGCGCGTGCTATGGCTGAATTAAATCATCCAAACATCGTTGCAATTAGAGATATAGGG
GAAGAGGACGGACAGCAGTTCCTAGTAATGGAATATGTTGATGGATTTGATCTTAAACGATTTATCCAGGAAAATGCACC
AATTCCAAATGAACAAGTTGTTAGAATCATGGAAGAAGTTTTATCTGCTATGACTTTAGCCCATCAAAAAGGGATTGTTC
ATAGAGATTTAAAACCTCAAAATATCCTGCTAACTAAGGATGGAGTTGTCAAAGTTACAGACTTTGGGATCGCAGTTGCC
TTTGCTGAGACAAGTTTAACTCAGACAAATTCAATGCTAGGTAGTGTTCACTATCTTTCACCTGAACAAGCAAGAGGTTC
AAAAGCGACTATCCAAAGTGATATTTATGCCATGGGTATTATGTTATTTGAAATGTTAACTGGTCATATTCCTTATGATG
GGGATAGTGCCGTAACAATTGCTTTACAACATTTCCAAAAACCACTGCCTTCAATTATTGAAGAAAATAAAGGTGTACCG
CAGGCTCTTGAGAATGTTGTTATTAAAGCGACAGCTAAGCGTTTAAGTGATCGATATGCGACTACTTTTGATATGAGCAG
AGATTTGATGACAGCGCTAAGCTATAATCGAAGCCGTGAACGGAAGTTAGTCTTCCATGATGTTGAAAGTACCAAGCCAC
TACCTAAAGTTACACCTGTTCCGGTTACGCAAACCAAATCATCTGTAGCACCTCAAACATCTGGATCTGAAATCCATGAT
GATGCGGTTACACCTCAAAAAACAAAGAAAAAACGTGGACGTCTTTTGGGAACTTTACTCAAAATTTTATTCGCCCTATT
TGTTGTTTTGGTTGCTGTCTTTGCCTTTTTAGTCCTAACGAAGCCATCTACGGTGAAGATTCCAAATGTGAATGGAACTA
CATTGAGTGTGGCCAAAAAAGAATTGACTGATTTAGGTTTCCAAGTTGGTCAGATTCACAAAATGGAAGATGCAGCAGTT
GATTCTGGTAAGGTCATTAAAACAGATCCTAAGGCAGGCACTGAGCGCCGTGAAGGGGCTCGGATTGATCTCTTTGTTTC
AACTGGACAACCTAAAGTTAAGAAGAAAAGTAAAGATAAAGTGTTAATGCCTGATTTTACGAATATGACTTACAAAGATG
TCCTTGATATGTTAACAAATGAGTATGGTATTCACCCAAATCGAATTGTTGCCTATTATCAAGATGCTCCGAATCCAGAA
AATTATTCCAAGGTAAGCTCACCAAAAGATTCACAACTTGTTATGGGACAATATCCAATAGTTGGTACTTATTACATTAT
TTCAGATAAGGAAGATATTGAACTTTATCTAAGTCCAGATTCTGGAGATAATACAACTGTATTCTCATCAACATCGACAT
CAAGTTCAAGTGAAGAGGAAACAAGTACAAGCACAGAAACTTCTACAAGCTCTGAATCAACAACAAGTTCAAGTAGTAGT
GATGCAACAACTACTGATTCAACGACTTCGGATACAAGTACTACTACACCTTAA

Domains

Predicted by InterproScan.

PASTA domain (PASTA)

(365-428)

PASTA domain (PASTA)

(441-518)

Protein kinase domain (Pkinase)

(12-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F1Z2Q9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

61.023

98.267

0.6
  stkP/pknB Streptococcus salivarius strain HSISS4

66.32

83.362

0.553
  stkP Streptococcus pneumoniae TIGR4

62.1

75.91

0.471
  stkP Streptococcus pneumoniae D39

61.872

75.91

0.47
  stkP Streptococcus pneumoniae R6

61.872

75.91

0.47
  stkP Streptococcus pneumoniae Rx1

61.644

75.91

0.468

Multiple sequence alignment