Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L0993_RS11785 Genome accession   NZ_CP090845
Coordinates   2684103..2685788 (+) Length   561 a.a.
NCBI ID   WP_373937773.1    Uniprot ID   -
Organism   Vibrio kanaloae strain WXL161     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2679103..2690788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0993_RS11765 (L0993_11760) pdhR 2680606..2681367 (-) 762 WP_017056615.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  L0993_RS11770 (L0993_11765) ampD 2681849..2682449 (-) 601 Protein_2266 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L0993_RS11775 (L0993_11770) nadC 2682533..2683423 (+) 891 WP_077680840.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L0993_RS11780 (L0993_11775) - 2683680..2684102 (+) 423 WP_373937771.1 pilin -
  L0993_RS11785 (L0993_11780) pilB 2684103..2685788 (+) 1686 WP_373937773.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L0993_RS11790 (L0993_11785) pilC 2685804..2687033 (+) 1230 WP_373937774.1 type II secretion system F family protein Machinery gene
  L0993_RS11795 (L0993_11790) pilD 2687101..2687970 (+) 870 WP_373937775.1 A24 family peptidase Machinery gene
  L0993_RS11800 (L0993_11795) coaE 2687973..2688587 (+) 615 WP_060468586.1 dephospho-CoA kinase -
  L0993_RS11805 (L0993_11800) zapD 2688614..2689354 (+) 741 WP_029627015.1 cell division protein ZapD -
  L0993_RS11810 (L0993_11805) yacG 2689435..2689632 (+) 198 WP_017056605.1 DNA gyrase inhibitor YacG -
  L0993_RS11815 (L0993_11810) rplS 2689858..2690211 (-) 354 WP_017056604.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62301.20 Da        Isoelectric Point: 5.1253

>NTDB_id=645171 L0993_RS11785 WP_373937773.1 2684103..2685788(+) (pilB) [Vibrio kanaloae strain WXL161]
MLTNLPTVLRQANFLSLTQEQAVAEYVQASGVSTPEALLVLDLFTGDSLANNIKTIFGLPLVQLANTDYETLCERLGLRE
LITKYCAIPIAVSSSTLTLASADPTDLHAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSIFGQDSKRKEITQDELAN
LVEVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEEQKALYLKALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLRGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKTVDDSPDIFLRHSIPNSQT
IYKANPQGCNECTQGYSGRVGIYEVMPFTDQLKSSLINKPNALTIESLARREGMRTLQESGLDKLLEGITSYQELQRVLY
I

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=645171 L0993_RS11785 WP_373937773.1 2684103..2685788(+) (pilB) [Vibrio kanaloae strain WXL161]
ATGCTCACCAACCTCCCAACCGTTCTACGTCAGGCTAATTTTCTTAGCCTGACTCAAGAACAAGCCGTTGCGGAATATGT
ACAAGCTTCCGGTGTTTCTACGCCTGAAGCTTTACTCGTTCTTGATTTATTCACCGGCGACTCTTTAGCAAACAATATCA
AAACCATCTTTGGGTTACCGTTAGTGCAACTCGCCAATACCGACTACGAAACCTTGTGTGAACGGCTAGGGCTTCGCGAG
CTGATCACCAAGTATTGTGCTATTCCGATTGCCGTTTCTAGTTCTACGCTCACGCTCGCCTCTGCCGACCCGACCGACTT
ACACGCTGAAGACGATTTCAGATTTGCGACAGGGTTGCAAATTGAACTCGTTGTCGCTAATTATTCAGAATTAGAAGGCG
CGATACGCAAACTGTATGGCCGCTCGATTTTCGGACAAGACTCCAAGCGAAAAGAGATCACTCAAGATGAACTCGCCAAC
CTAGTTGAAGTCTCTGACGATGAAATCACGTCAATTGAAGATCTCAGCCAAGATGACTCCCCTGTGAGCCGTTTTATCAA
CCAAATATTGGTTGATGCGGTGCGTAAAGGCGCCTCTGATATCCACTTCGAACCTTATGAAGAAAACTATCGAGTTCGCC
TGCGTTGCGATGGCATCCTTGTCGAAATTCAACAGCCCGCTTCTCATTTAAGTCGTCGGTTATCCGCCCGCTTAAAAATT
CTCGCTAAGTTGGATATTGCCGAGCGTCGATTGCCTCAAGACGGCCGCATCAAGTTGCGGTTAAATGATGAGCTTGCGAT
TGATATGCGTGTATCGACGCTACCGACATTATGGGGAGAGAAGATCGTACTGCGTCTGCTAGACAGTAGCGCTGCCAATT
TAGATATCGATAAACTCGGCTACAGCGAAGAACAAAAAGCACTCTACCTTAAGGCACTGAAACGACCGCAAGGGATGATT
TTAATGACCGGGCCAACCGGCAGTGGCAAAACAGTCTCCCTTTACACTGGCCTTCGAGTTCTCAACACCACAGAACGCAA
CATCTCTACCGCAGAAGATCCGGTCGAAATTAACTTACGTGGTATCAACCAAGTACAAGTCACACCAAAGATCGGCTTTG
GGTTTGCTGAAGCACTACGATCCTTTTTACGGCAAGACCCAGACATCGTGATGGTCGGAGAGATCCGTGACTTAGAAACG
GCAGAGATAGCGATCAAAGCCTCGCAAACTGGCCACTTAGTTCTTTCAACACTGCATACAAACTCTGCAGCAGAAACCGT
CACTCGACTCGCTCACATGGGGATAGAGCCCTTTAACCTTGCTTCATCATTAAGCCTAATTATTGCTCAGCGACTCGCGA
GACGCTTGTGCAACCATTGCAAAACGGTGGATGATTCACCGGACATATTTCTGCGTCACTCTATTCCCAACAGCCAAACC
ATTTACAAAGCCAACCCACAAGGATGCAATGAGTGTACTCAAGGGTATTCTGGCCGAGTCGGTATCTACGAAGTGATGCC
GTTTACCGACCAACTCAAAAGCAGCCTTATCAACAAGCCTAACGCGCTAACAATTGAAAGCCTTGCTCGTCGGGAAGGTA
TGCGAACTCTACAAGAATCAGGGCTTGATAAGCTGCTTGAAGGTATCACCAGCTACCAAGAACTACAGCGTGTTCTGTAC
ATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.227

100

0.736
  pilB Vibrio campbellii strain DS40M4

70.463

100

0.706
  pilB Vibrio parahaemolyticus RIMD 2210633

69.929

100

0.701
  pilB Acinetobacter baumannii D1279779

51.252

92.513

0.474
  pilB Legionella pneumophila strain ERS1305867

50.775

91.979

0.467
  pilB Acinetobacter baylyi ADP1

49.423

92.692

0.458
  pilF Neisseria gonorrhoeae MS11

43.898

97.861

0.43
  pilF Thermus thermophilus HB27

39.155

92.87

0.364