Detailed information NTG0645056

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LT978_RS07095
• Genome accession: NZ_CP090838
• Coordinates: 1385132..1385746 (+)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain TL106
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1380132..1390746

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LT978_RS07060 (LT978_07055)	aroE	1380819..1381661 (+)	843	WP_025852995.1	shikimate dehydrogenase	-
LT978_RS07065 (LT978_07060)	yhbY	1381655..1381945 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
LT978_RS07070 (LT978_07065)	-	1381956..1382525 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
LT978_RS07075 (LT978_07070)	yqeK	1382515..1383096 (+)	582	WP_057080474.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LT978_RS07080 (LT978_07075)	rsfS	1383093..1383449 (+)	357	WP_003152864.1	ribosome silencing factor	-
LT978_RS07085 (LT978_07080)	-	1383446..1384183 (+)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
LT978_RS07090 (LT978_07085)	comER	1384252..1385073 (-)	822	WP_139890042.1	late competence protein ComER	-
LT978_RS07095 (LT978_07090)	comEA	1385132..1385746 (+)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
LT978_RS07100 (LT978_07095)	-	1385813..1386382 (+)	570	WP_003152868.1	ComE operon protein 2	-
LT978_RS07105 (LT978_07100)	comEC	1386383..1388734 (+)	2352	WP_139890043.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LT978_RS07110 (LT978_07105)	-	1388753..1388887 (-)	135	WP_003152870.1	YqzM family protein	-
LT978_RS07115 (LT978_07110)	-	1388928..1389082 (+)	155	Protein_1399	hypothetical protein	-
LT978_RS07120 (LT978_07115)	holA	1389122..1390163 (+)	1042	Protein_1400	DNA polymerase III subunit delta	-
LT978_RS07125 (LT978_07120)	rpsT	1390180..1390446 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=645056 LT978_RS07095 WP_043867297.1 1385132..1385746(+) (comEA) [Bacillus amyloliquefaciens strain TL106]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=645056 LT978_RS07095 WP_043867297.1 1385132..1385746(+) (comEA) [Bacillus amyloliquefaciens strain TL106]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363