Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L0F67_RS10545 Genome accession   NZ_CP090556
Coordinates   2176433..2176870 (-) Length   145 a.a.
NCBI ID   WP_014991735.1    Uniprot ID   -
Organism   Actinobacillus suis strain D20_277     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2171433..2181870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0F67_RS10520 (L0F67_10505) yacG 2172337..2172525 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  L0F67_RS10525 (L0F67_10510) coaE 2172507..2173145 (-) 639 WP_014991731.1 dephospho-CoA kinase -
  L0F67_RS10530 (L0F67_10515) - 2173167..2173841 (-) 675 WP_014991732.1 fimbrial protein -
  L0F67_RS10535 (L0F67_10520) - 2173841..2175034 (-) 1194 WP_014991733.1 type II secretion system F family protein -
  L0F67_RS10540 (L0F67_10525) pilB 2175027..2176412 (-) 1386 WP_014991734.1 GspE/PulE family protein Machinery gene
  L0F67_RS10545 (L0F67_10530) pilA 2176433..2176870 (-) 438 WP_014991735.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  L0F67_RS10550 (L0F67_10535) radA 2177050..2178428 (+) 1379 Protein_2026 DNA repair protein RadA -
  L0F67_RS10555 (L0F67_10540) pepB 2178494..2179776 (+) 1283 Protein_2027 aminopeptidase PepB -
  L0F67_RS10560 (L0F67_10545) - 2179904..2180838 (+) 935 Protein_2028 SIS domain-containing protein -
  L0F67_RS10565 (L0F67_10550) greB 2180848..2181336 (+) 489 WP_014991739.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15395.62 Da        Isoelectric Point: 7.6225

>NTDB_id=643423 L0F67_RS10545 WP_014991735.1 2176433..2176870(-) (pilA) [Actinobacillus suis strain D20_277]
MKHIIKPIQQAFTLIELMIVIAIIAILATVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGATNSCFSGANGIKN
GDDLKNSKYLDSVSVTAGTITVNGKGSVAGYGYTLTPDYSSKTLTWKTTCSGEDKSLFPAAFCSE

Nucleotide


Download         Length: 438 bp        

>NTDB_id=643423 L0F67_RS10545 WP_014991735.1 2176433..2176870(-) (pilA) [Actinobacillus suis strain D20_277]
ATGAAACACATAATTAAACCGATACAACAGGCATTTACCCTGATTGAATTAATGATTGTGATTGCAATCATTGCAATTTT
AGCAACTGTGGCGATTCCTTCTTATAACAATTACACCAAAAAAGCGGCATTATCCGAACTGTTAGCGGCATCTTCTGCAT
ATAAAACGGATGTAGAAATTTGTATTTATAACACTGGAGCTACCAATAGCTGTTTTAGTGGAGCCAATGGCATTAAGAAT
GGCGATGATCTTAAAAATTCTAAGTATTTAGATTCAGTCTCTGTAACTGCCGGTACTATAACCGTGAATGGCAAGGGCAG
CGTAGCGGGATATGGTTATACACTTACACCGGATTATTCAAGTAAAACACTGACGTGGAAAACAACCTGTTCAGGAGAGG
ATAAAAGTTTATTCCCCGCAGCATTTTGCTCGGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(9-31)

Pilin (bacterial filament) (Pilin)

(41-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

60.274

100

0.607
  pilA Haemophilus influenzae Rd KW20

56.738

97.241

0.552
  pilA Haemophilus influenzae 86-028NP

56.429

96.552

0.545
  comP Acinetobacter baylyi ADP1

42.254

97.931

0.414
  pilA/pilA1 Eikenella corrodens VA1

36.552

100

0.366