Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L0F67_RS10540 Genome accession   NZ_CP090556
Coordinates   2175027..2176412 (-) Length   461 a.a.
NCBI ID   WP_014991734.1    Uniprot ID   K0G4F5
Organism   Actinobacillus suis strain D20_277     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2170027..2181412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0F67_RS10510 (L0F67_10495) lptE 2170635..2171129 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  L0F67_RS10515 (L0F67_10500) holA 2171235..2172260 (+) 1026 WP_014991730.1 DNA polymerase III subunit delta -
  L0F67_RS10520 (L0F67_10505) yacG 2172337..2172525 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  L0F67_RS10525 (L0F67_10510) coaE 2172507..2173145 (-) 639 WP_014991731.1 dephospho-CoA kinase -
  L0F67_RS10530 (L0F67_10515) - 2173167..2173841 (-) 675 WP_014991732.1 fimbrial protein -
  L0F67_RS10535 (L0F67_10520) - 2173841..2175034 (-) 1194 WP_014991733.1 type II secretion system F family protein -
  L0F67_RS10540 (L0F67_10525) pilB 2175027..2176412 (-) 1386 WP_014991734.1 GspE/PulE family protein Machinery gene
  L0F67_RS10545 (L0F67_10530) pilA 2176433..2176870 (-) 438 WP_014991735.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  L0F67_RS10550 (L0F67_10535) radA 2177050..2178428 (+) 1379 Protein_2026 DNA repair protein RadA -
  L0F67_RS10555 (L0F67_10540) pepB 2178494..2179776 (+) 1283 Protein_2027 aminopeptidase PepB -
  L0F67_RS10560 (L0F67_10545) - 2179904..2180838 (+) 935 Protein_2028 SIS domain-containing protein -
  L0F67_RS10565 (L0F67_10550) greB 2180848..2181336 (+) 489 WP_014991739.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52498.04 Da        Isoelectric Point: 5.3482

>NTDB_id=643422 L0F67_RS10540 WP_014991734.1 2175027..2176412(-) (pilB) [Actinobacillus suis strain D20_277]
MQYSVCDMNSQQVFDISESLWQKNCDEKHILLRYLAIPIQENDDTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSLYEETELNYQSPDNLDLLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLQIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLTALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDQESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIKNSLQLIIAQRLLRKRCPQCGGKGC
DNCYHGYKGRTGIYQLLAKQGKFFEKSTAYLDYDNLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=643422 L0F67_RS10540 WP_014991734.1 2175027..2176412(-) (pilB) [Actinobacillus suis strain D20_277]
ATGCAGTATTCGGTTTGTGATATGAATAGCCAGCAGGTATTTGATATCTCTGAATCCTTGTGGCAGAAAAATTGTGACGA
AAAACATATTTTGTTGCGTTATTTAGCCATTCCGATTCAAGAAAATGACGATACGTTATGGCTTGCAATTGATGATATGA
ATAATTTAAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAATACTTGCTTAATCAACTTTCACCGGAACAGCAATCACTATACGAGGAAACTGAGTTAAATTATCAATCGCCGGATAA
TTTAGATCTACTCAATCAAAATGATCCCATTATTCAGATTTTAGATAATTTATTTAAATTCTGCTTAAAGCAAAATGCTT
CGGACATTCACTTTGAACCGCAAAAAGAGAAGCTACAGATTAGATTGCGTATTGATGGGGTATTGCATATCTATAAAAGT
TTATCGTTTAGTTTAGCTAATCGTATTATCACGAGGCTTAAATTGTTAGCTAAATTAGATATTAGCGAAACACGTTTACC
ACAAGATGGCCAATTTAATTTTAAGACGGTAACGGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCCACGAGCTTTG
GTGAAAAAATCGTTTTACGTTTACAAAAGAATAAACCTACTCATTTCGATTTTTTGGAATTAGGTTTCTCACCGGAACAA
AAACAATTACTACTTACGGCACTACATCAACCACAAGGGCTGATTCTGGTGACAGGACCAACGGGCAGTGGTAAAAGTAT
TACGTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACGGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTAAATCGTGCAATTCAATTAGACTTTAGCCAATTACTCAGAACATTTTTACGACAA
GATCCGGACATTATTATGTTAGGAGAAATTCGTGATCAAGAAAGTGCTGAAATTGCATTAAGAGCAGCACAAACAGGGCA
TCTAGTGCTTTCTACATTACATACGAATGATGCACCGTCTGCGATTGAGCGTTTACTACAATTAGGGATTAAAGAGTATG
AGATTAAGAATTCGCTGCAACTGATTATCGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGATGT
GATAACTGTTATCACGGTTATAAAGGGCGAACGGGAATTTACCAATTACTTGCAAAACAAGGTAAATTTTTTGAAAAATC
GACCGCTTATTTGGATTATGACAATTTACGAGCCAGTGCTGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K0G4F5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.489

99.349

0.72
  pilB Haemophilus influenzae Rd KW20

57.65

97.831

0.564
  pilB Haemophilus influenzae 86-028NP

56.984

97.831

0.557
  pilB Legionella pneumophila strain ERS1305867

39.634

100

0.423
  pilB Vibrio parahaemolyticus RIMD 2210633

40.333

100

0.421
  pilB Vibrio campbellii strain DS40M4

40

100

0.421
  pilB Acinetobacter baylyi ADP1

38.171

100

0.416
  pilB Vibrio cholerae strain A1552

38.554

100

0.416
  pilB Acinetobacter baumannii D1279779

38.508

100

0.414