Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IHC89_RS02330 Genome accession   NZ_CP090495
Coordinates   462711..463817 (+) Length   368 a.a.
NCBI ID   WP_005297691.1    Uniprot ID   A0A2T3I981
Organism   Photobacterium damselae subsp. damselae strain AS-15-0759-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 457711..468817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHC89_RS02300 (IHC89_02300) - 458547..459140 (-) 594 WP_044174415.1 XTP/dITP diphosphatase -
  IHC89_RS02305 (IHC89_02305) yggU 459242..459541 (-) 300 WP_036763214.1 DUF167 family protein YggU -
  IHC89_RS02310 (IHC89_02310) - 459538..460095 (-) 558 WP_005297682.1 YggT family protein -
  IHC89_RS02315 (IHC89_02315) proC 460107..460925 (-) 819 WP_237031338.1 pyrroline-5-carboxylate reductase -
  IHC89_RS02320 (IHC89_02320) - 460941..461642 (-) 702 WP_005297686.1 YggS family pyridoxal phosphate-dependent enzyme -
  IHC89_RS02325 (IHC89_02325) pilT 461665..462699 (+) 1035 WP_005297689.1 type IV pilus twitching motility protein PilT Machinery gene
  IHC89_RS02330 (IHC89_02330) pilU 462711..463817 (+) 1107 WP_005297691.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IHC89_RS02335 (IHC89_02335) ruvX 464134..464559 (-) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  IHC89_RS02340 (IHC89_02340) - 464556..465119 (-) 564 WP_068945809.1 YqgE/AlgH family protein -
  IHC89_RS02345 (IHC89_02345) gshB 465273..466220 (-) 948 WP_237031339.1 glutathione synthase -
  IHC89_RS02350 (IHC89_02350) rsmE 466241..466972 (-) 732 WP_005297699.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IHC89_RS02355 (IHC89_02355) endA 467104..467808 (-) 705 WP_005297700.1 deoxyribonuclease I -
  IHC89_RS02360 (IHC89_02360) - 467939..468421 (-) 483 WP_237031340.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41354.51 Da        Isoelectric Point: 5.5884

>NTDB_id=642901 IHC89_RS02330 WP_005297691.1 462711..463817(+) (pilU) [Photobacterium damselae subsp. damselae strain AS-15-0759-2]
MKLQEILNQVVERKASDLYITVDSPCLLKVDGVLHPIGDTLDRTGVDLLFDEAMDSALYQEFVDTREANFALVRGDWRFR
VSAFWQRELPGMVVRRIETDIPDIFSLKLPMDMQKMALAKRGLVLVVGATGSGKSTSMAAMTGFRNQNRSGHILTVEDPI
EFVHKHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTQETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERQHQFLFDLSLNLKCILAQQLIPDAHGQGRHAAFELLINTPRVADLIRRGELYEIKDVMAKSGESGMMTFDQSLY
ELFTKGAITEQDALHHADSPNDLRLMIKVGSKDKTAINALDGVTVDFN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=642901 IHC89_RS02330 WP_005297691.1 462711..463817(+) (pilU) [Photobacterium damselae subsp. damselae strain AS-15-0759-2]
ATGAAACTACAAGAAATTTTAAATCAGGTTGTTGAACGTAAAGCGTCGGATCTCTATATCACCGTGGATTCTCCGTGCCT
ATTAAAAGTGGATGGTGTGTTGCATCCCATTGGCGATACGTTAGATCGTACAGGGGTTGATTTACTGTTTGATGAGGCGA
TGGACAGTGCTTTATATCAAGAGTTTGTTGATACCCGAGAAGCGAATTTTGCTTTAGTTCGTGGTGATTGGCGGTTTCGT
GTCAGTGCGTTTTGGCAGCGAGAATTACCCGGCATGGTTGTTCGTCGGATTGAAACCGATATTCCAGATATCTTTAGCCT
GAAATTGCCGATGGATATGCAAAAAATGGCTCTAGCTAAACGCGGTTTAGTTTTAGTGGTTGGAGCAACGGGGTCGGGTA
AATCGACTTCGATGGCAGCAATGACAGGGTTTCGAAATCAAAATCGCAGTGGCCATATTTTAACGGTTGAAGATCCGATT
GAATTTGTCCACAAACACGATAAATGCATAGTGACCCAGCGTGAAGTTGGTCTCGATACTGAGAGTTATGAAGTGGCGCT
TAAGAACTCTTTGCGACAAGCGCCTGATATGATCTTAATCGGCGAAATTCGTACGCAAGAGACGATGGAATATGCGATGA
ATTTTGCGGAAACGGGTCATTTATGTATGGCCACATTGCACGCCAATAATGCTAACCAAGCGTTAGAGCGTATTCTGCAT
CTGGTACCGAAAGAGCGCCAACATCAGTTTTTGTTTGATTTATCCTTGAACCTTAAATGCATTTTAGCTCAGCAACTTAT
CCCTGATGCTCATGGTCAAGGACGGCATGCTGCCTTTGAACTGTTAATTAATACACCGCGAGTGGCGGATCTTATTCGTC
GTGGTGAACTTTATGAGATCAAAGATGTGATGGCCAAATCAGGTGAATCGGGAATGATGACTTTTGATCAATCGTTGTAT
GAGTTATTCACTAAAGGTGCCATTACAGAACAAGATGCTCTTCATCATGCGGATTCTCCAAATGATTTACGTTTAATGAT
CAAAGTAGGGAGTAAAGATAAAACGGCGATCAATGCGTTGGATGGGGTAACGGTAGATTTTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3I981

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.12

99.728

0.679
  pilU Pseudomonas stutzeri DSM 10701

59.497

97.283

0.579
  pilU Acinetobacter baylyi ADP1

55.211

96.467

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.449

93.75

0.389
  pilT Legionella pneumophila strain Lp02

40.299

91.033

0.367
  pilT Legionella pneumophila strain ERS1305867

40.299

91.033

0.367