Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IHC93_RS02290 Genome accession   NZ_CP090485
Coordinates   451679..452785 (+) Length   368 a.a.
NCBI ID   WP_237041301.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain AS-16-0963-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 446679..457785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHC93_RS02260 (IHC93_02260) - 447515..448108 (-) 594 WP_044174415.1 XTP/dITP diphosphatase -
  IHC93_RS02265 (IHC93_02265) yggU 448210..448509 (-) 300 WP_036763214.1 DUF167 family protein YggU -
  IHC93_RS02270 (IHC93_02270) - 448506..449063 (-) 558 WP_005297682.1 YggT family protein -
  IHC93_RS02275 (IHC93_02275) proC 449075..449893 (-) 819 WP_005297684.1 pyrroline-5-carboxylate reductase -
  IHC93_RS02280 (IHC93_02280) - 449909..450610 (-) 702 WP_005297686.1 YggS family pyridoxal phosphate-dependent enzyme -
  IHC93_RS02285 (IHC93_02285) pilT 450633..451667 (+) 1035 WP_005297689.1 type IV pilus twitching motility protein PilT Machinery gene
  IHC93_RS02290 (IHC93_02290) pilU 451679..452785 (+) 1107 WP_237041301.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IHC93_RS02295 (IHC93_02295) ruvX 453120..453545 (-) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  IHC93_RS02300 (IHC93_02300) - 453542..454105 (-) 564 WP_068945809.1 YqgE/AlgH family protein -
  IHC93_RS02305 (IHC93_02305) gshB 454259..455206 (-) 948 WP_005297697.1 glutathione synthase -
  IHC93_RS02310 (IHC93_02310) rsmE 455227..455958 (-) 732 WP_237041302.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IHC93_RS02315 (IHC93_02315) endA 456093..456797 (-) 705 WP_005297700.1 deoxyribonuclease I -
  IHC93_RS02320 (IHC93_02320) - 456928..457410 (-) 483 WP_036763215.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41354.51 Da        Isoelectric Point: 5.5884

>NTDB_id=642761 IHC93_RS02290 WP_237041301.1 451679..452785(+) (pilU) [Photobacterium damselae subsp. damselae strain AS-16-0963-1]
MKLQEILNQVVERKASDLYITVDSPCLLKVDGVLHPIGDTLDRTGVDLLFDEAMDSALYQEFVDTREANFALVRGDWRFR
VSAFWQRELPGMVVRRIETDIPDIFSLKLPMDMQKMALAKRGLVLVVGATGSGKSTSMAAMTGFRNQNRSGHILTVEDPI
EFVHKHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTQETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERQHQFLFDLSLNLKCILAQQLIPDAHGQGRHAAFELLINTPRVADLIRRGELYELKDVMAKSGESGMMTFDQSLY
ELFTKGAITEQDALHHADSPNDLRLMIKVGSKDKTAINALDGVTVDFN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=642761 IHC93_RS02290 WP_237041301.1 451679..452785(+) (pilU) [Photobacterium damselae subsp. damselae strain AS-16-0963-1]
ATGAAACTACAAGAAATTTTAAATCAGGTTGTTGAACGTAAAGCGTCGGATCTCTATATCACCGTGGATTCTCCGTGCCT
ATTAAAAGTGGATGGTGTGTTGCATCCCATTGGCGATACGTTAGATCGTACAGGGGTTGATTTACTGTTCGATGAGGCGA
TGGACAGTGCTTTATATCAAGAGTTTGTTGATACCCGAGAAGCGAATTTTGCTTTAGTTCGGGGCGATTGGCGGTTTCGT
GTCAGTGCGTTTTGGCAGCGAGAATTACCCGGCATGGTTGTTCGTCGGATTGAAACCGATATTCCAGATATCTTTAGCTT
GAAATTGCCGATGGATATGCAAAAAATGGCTCTAGCTAAACGCGGTTTAGTTTTAGTGGTTGGTGCAACGGGGTCGGGTA
AATCGACTTCGATGGCGGCAATGACCGGGTTTCGAAATCAAAATCGCAGTGGCCATATTTTAACGGTTGAAGATCCGATT
GAATTTGTCCACAAACACGATAAATGCATAGTGACTCAGCGTGAAGTTGGTCTCGATACTGAGAGTTATGAAGTGGCGCT
TAAGAACTCTTTGCGACAAGCGCCTGATATGATCTTAATCGGCGAAATTCGTACGCAAGAGACGATGGAATATGCGATGA
ATTTTGCGGAAACGGGTCATTTATGTATGGCCACATTGCACGCCAATAATGCTAACCAAGCGTTAGAGCGTATTCTGCAT
CTGGTACCGAAAGAGCGCCAACATCAGTTTTTGTTTGATTTATCCTTGAACCTTAAATGCATTTTAGCTCAGCAACTTAT
CCCTGATGCTCATGGTCAAGGACGGCATGCTGCCTTTGAACTGTTAATTAATACACCGCGAGTGGCGGATCTTATTCGTC
GTGGTGAACTTTATGAGCTCAAAGATGTGATGGCCAAATCAGGTGAATCGGGAATGATGACTTTTGATCAATCGTTGTAT
GAGTTATTCACTAAAGGTGCCATTACAGAACAAGATGCTCTTCATCATGCGGATTCTCCAAATGATTTACGTTTAATGAT
CAAAGTAGGGAGTAAAGATAAAACGGCGATCAATGCGTTGGATGGGGTAACGGTAGATTTTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.392

99.728

0.682
  pilU Pseudomonas stutzeri DSM 10701

59.777

97.283

0.582
  pilU Acinetobacter baylyi ADP1

54.93

96.467

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.159

93.75

0.386
  pilT Legionella pneumophila strain Lp02

40.299

91.033

0.367
  pilT Legionella pneumophila strain ERS1305867

40.299

91.033

0.367