Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LY387_RS14205 Genome accession   NZ_CP090438
Coordinates   3130932..3132617 (+) Length   561 a.a.
NCBI ID   WP_419153450.1    Uniprot ID   -
Organism   Vibrio maritimus strain BH16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3125932..3137617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LY387_RS14185 pdhR 3127446..3128213 (-) 768 WP_042475867.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  LY387_RS14190 ampD 3128624..3129205 (-) 582 WP_234494571.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LY387_RS14195 nadC 3129340..3130230 (+) 891 WP_234494573.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LY387_RS14200 - 3130492..3130923 (+) 432 WP_234494575.1 pilin -
  LY387_RS14205 pilB 3130932..3132617 (+) 1686 WP_419153450.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LY387_RS14210 pilC 3132630..3133856 (+) 1227 WP_234496130.1 type II secretion system F family protein Machinery gene
  LY387_RS14215 pilD 3133899..3134771 (+) 873 WP_234494577.1 prepilin peptidase Machinery gene
  LY387_RS14220 coaE 3134771..3135379 (+) 609 WP_128647939.1 dephospho-CoA kinase -
  LY387_RS14225 zapD 3135411..3136151 (+) 741 WP_234494579.1 cell division protein ZapD -
  LY387_RS14230 yacG 3136188..3136382 (+) 195 WP_042475855.1 DNA gyrase inhibitor YacG -
  LY387_RS14235 rplS 3136491..3136844 (-) 354 WP_112478814.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62142.50 Da        Isoelectric Point: 6.1271

>NTDB_id=642450 LY387_RS14205 WP_419153450.1 3130932..3132617(+) (pilB) [Vibrio maritimus strain BH16]
MSRLPSVLAEAQIISESQKDRLIECTQSSMESVPSIIVELGWLTAQDLTSALEKLFALPAKQLHDYDYIELCRTAGLREL
IVKNHAIPLKQSSHTLSIAISDPSIVELENDFRFATGLQIELVICDCLDITSAIKRLYGENHKGINPSFRELTSEALTNL
VPISDSELENVEDLSQDDSPVSRFIHQVLTDAVRKKASDIHFEPYEHSYRIRLRCDGILIETHQPATQLSRRLAARIKIL
AKLDIAERRLPQDGRLKLKLSEAASIDMRVSTLPTMWGEKIVLRLLDADATQLNIDQLGYTEQQKSLYLTALKKPQGMIL
MTGPTGSGKTVSLYTGLSILNTAERNISTAEDPVEIHLEGINQVQINPKIGFDFSTALRSFLRQDPDIVMLGEIRDIETA
EIAVKAAQTGHLVLSTLHTNSAAETVIRLKNMGVDAINLSSSLSLIIAQRLARKLCECCKIPDPMSPKLRHSLNIPNESI
VYLANEQGCNHCNHGFLGRTGIYEVMAFTHELASAIVASASLNEIEAIAKAQGMKTLKQSGVEKLIAGITSYSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=642450 LY387_RS14205 WP_419153450.1 3130932..3132617(+) (pilB) [Vibrio maritimus strain BH16]
ATAAGTAGGCTGCCTAGCGTTCTTGCTGAGGCTCAAATTATTTCAGAGAGTCAAAAAGATCGTCTAATAGAATGCACGCA
ATCAAGTATGGAGTCTGTTCCATCAATCATTGTGGAATTGGGCTGGCTGACAGCCCAAGACTTAACTTCGGCTTTAGAGA
AACTATTTGCTCTGCCCGCTAAACAACTGCATGACTACGACTACATAGAGCTGTGTCGAACAGCAGGATTACGCGAGCTA
ATTGTAAAAAACCACGCAATTCCGCTCAAGCAAAGTAGCCACACCCTCTCTATCGCAATATCCGACCCGAGCATTGTCGA
GTTAGAAAACGATTTTCGTTTTGCGACCGGACTACAGATAGAGCTCGTCATCTGTGATTGCCTTGATATCACATCGGCCA
TTAAGCGTCTTTATGGCGAAAACCATAAGGGCATCAACCCTAGCTTTAGAGAGCTCACTTCTGAAGCACTAACCAACCTA
GTCCCAATCAGCGATTCGGAGCTCGAGAACGTCGAAGACCTCTCCCAAGATGACTCTCCTGTAAGTCGCTTTATTCATCA
GGTATTGACGGATGCCGTGCGCAAGAAAGCCTCGGATATTCATTTCGAACCTTATGAGCACTCTTATCGAATACGGCTTC
GCTGTGATGGCATCTTGATAGAGACTCACCAGCCTGCGACGCAGCTTAGTCGTCGATTGGCAGCTCGGATTAAGATCTTG
GCTAAACTTGATATCGCCGAAAGGCGCTTACCACAAGACGGTCGACTGAAACTGAAACTCTCTGAAGCAGCCTCTATCGA
CATGCGCGTTTCAACCCTACCCACAATGTGGGGTGAGAAAATCGTGTTAAGACTTTTGGACGCCGATGCCACCCAGCTCA
ACATTGATCAGCTAGGTTATACCGAGCAACAAAAATCGCTTTACCTCACCGCACTAAAAAAGCCCCAAGGTATGATCCTA
ATGACAGGTCCCACTGGCAGTGGAAAAACCGTTTCGCTATACACTGGACTTAGCATTCTCAATACCGCAGAGCGCAATAT
TTCTACTGCTGAAGATCCAGTTGAGATTCATCTTGAGGGCATCAATCAAGTTCAGATCAATCCAAAGATCGGCTTTGATT
TCTCTACCGCCCTTCGATCTTTTTTAAGACAAGATCCCGATATTGTCATGCTCGGCGAAATCCGAGATATCGAAACCGCC
GAAATTGCGGTCAAAGCCGCTCAAACGGGCCATCTAGTGTTATCTACACTGCACACCAACTCGGCCGCAGAGACCGTCAT
TAGGCTCAAAAACATGGGTGTCGACGCTATCAACTTGTCGTCCTCTCTATCGTTGATCATTGCTCAGCGCTTAGCACGAA
AGCTGTGTGAGTGTTGTAAGATACCAGACCCAATGTCCCCTAAACTTCGTCACTCCCTCAACATTCCCAATGAGTCGATA
GTCTACCTCGCCAACGAGCAAGGCTGTAACCATTGTAATCATGGTTTTCTCGGTAGGACTGGTATCTATGAGGTAATGGC
GTTTACCCATGAACTCGCCAGCGCGATAGTTGCTAGCGCAAGCCTAAATGAAATAGAAGCTATTGCTAAGGCTCAGGGAA
TGAAGACGCTAAAGCAATCTGGTGTGGAAAAGCTAATCGCTGGTATTACTAGCTACAGCGAGTTGCAACGCGTGCTCTAC
TTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

64.769

100

0.649
  pilB Vibrio parahaemolyticus RIMD 2210633

61.607

99.822

0.615
  pilB Vibrio campbellii strain DS40M4

61.497

100

0.615
  pilB Acinetobacter baumannii D1279779

45.31

100

0.456
  pilB Acinetobacter baylyi ADP1

45.063

99.287

0.447
  pilB Legionella pneumophila strain ERS1305867

49.693

87.166

0.433
  pilF Neisseria gonorrhoeae MS11

42.446

99.109

0.421