Detailed information NTG0064209

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: U722_RS12165
• Genome accession: NC_023073
• Coordinates: 2586690..2587304 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens LFB112
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2581690..2592304

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U722_RS12140 (U722_12940)	rpsT	2581990..2582256 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
U722_RS12145 (U722_12945)	holA	2582273..2583314 (-)	1042	Protein_2408	DNA polymerase III subunit delta	-
U722_RS19875	-	2583354..2583508 (-)	155	Protein_2409	hypothetical protein	-
U722_RS19480 (U722_12950)	-	2583549..2583683 (+)	135	WP_003152870.1	YqzM family protein	-
U722_RS12155 (U722_12955)	comEC	2583702..2586053 (-)	2352	WP_024085624.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
U722_RS12160 (U722_12960)	-	2586054..2586623 (-)	570	WP_003152868.1	ComE operon protein 2	-
U722_RS12165 (U722_12965)	comEA	2586690..2587304 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
U722_RS12170 (U722_12970)	comER	2587363..2588184 (+)	822	WP_014305449.1	late competence protein ComER	-
U722_RS12175 (U722_12975)	-	2588253..2588990 (-)	738	WP_024085626.1	class I SAM-dependent DNA methyltransferase	-
U722_RS12180 (U722_12980)	rsfS	2588987..2589343 (-)	357	WP_003152864.1	ribosome silencing factor	-
U722_RS12185 (U722_12985)	yqeK	2589361..2589921 (-)	561	WP_024085627.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
U722_RS12190 (U722_12990)	-	2589911..2590480 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
U722_RS12195 (U722_12995)	yhbY	2590491..2590781 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
U722_RS12200 (U722_13000)	aroE	2590775..2591617 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=64209 U722_RS12165 WP_041482377.1 2586690..2587304(-) (comEA) [Bacillus amyloliquefaciens LFB112]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=64209 U722_RS12165 WP_041482377.1 2586690..2587304(-) (comEA) [Bacillus amyloliquefaciens LFB112]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363