Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LVB87_RS01795 Genome accession   NZ_CP089925
Coordinates   369586..371310 (+) Length   574 a.a.
NCBI ID   WP_232899219.1    Uniprot ID   -
Organism   Lysobacter sp. KIS68-7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 364586..376310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVB87_RS01775 (LVB87_01775) - 365739..366728 (+) 990 WP_232899215.1 glycosyltransferase family 4 protein -
  LVB87_RS01780 (LVB87_01780) - 366725..367468 (-) 744 WP_232899216.1 class I SAM-dependent methyltransferase -
  LVB87_RS01785 (LVB87_01785) - 367465..368601 (-) 1137 WP_232899217.1 glycosyltransferase family 4 protein -
  LVB87_RS01790 (LVB87_01790) - 368613..369464 (-) 852 WP_232899218.1 glycosyltransferase family 2 protein -
  LVB87_RS01795 (LVB87_01795) pilB 369586..371310 (+) 1725 WP_232899219.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVB87_RS01800 (LVB87_01800) pilC 371406..372605 (+) 1200 WP_232900412.1 type II secretion system F family protein Machinery gene
  LVB87_RS01805 (LVB87_01805) - 372606..373472 (+) 867 WP_232899220.1 A24 family peptidase -
  LVB87_RS01810 (LVB87_01810) coaE 373477..374082 (+) 606 WP_232899221.1 dephospho-CoA kinase -
  LVB87_RS01815 (LVB87_01815) - 374086..375057 (-) 972 WP_232899222.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62643.85 Da        Isoelectric Point: 6.1639

>NTDB_id=639127 LVB87_RS01795 WP_232899219.1 369586..371310(+) (pilB) [Lysobacter sp. KIS68-7]
MATVATANLVGITGIARRLVMDGALDEAAARGAMETATRERKPLALFMAEQKLVTSAQLAAANSAEFGMPIFDVTALDPN
QSAIKLVSEELVRKHAALPLFKRGGRLFVGISDPTNSHALDEIKFHTNLAVEPILVDDDKIRRTIDHWLEQADALADAVA
DSEGLDNLDTSGGDEEFNNDSGVDAKGDDTPVVKFINKVLVDAIRRGASDIHFEPYETDYRVRLRIDGLLKQVARVPVKL
QARIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGGAAKLGIDKLGYEEDQKKLFL
EAVQKPYGMVLVTGPTGSGKTVSLYTALNILNDDTRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDVI
MVGEIRDLETAEIGIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREVHLPDHAL
LAEGFTSDEIHGGMKVYEAVGCPDCTEGYKGRTGIYQVMPMSEEIQDIVLSGGNVMQIGEAAQRSGVRDLRQSALVKVRN
GVTSLAEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=639127 LVB87_RS01795 WP_232899219.1 369586..371310(+) (pilB) [Lysobacter sp. KIS68-7]
ATGGCGACCGTTGCGACCGCCAACCTGGTGGGCATCACCGGCATCGCCCGGCGCTTGGTCATGGACGGCGCCCTGGACGA
AGCTGCCGCCCGCGGGGCGATGGAAACGGCGACCCGCGAGCGCAAGCCGCTGGCGCTGTTCATGGCCGAGCAGAAACTGG
TGACGTCGGCCCAGCTGGCCGCGGCCAATTCGGCCGAGTTCGGCATGCCGATCTTCGACGTGACCGCGCTCGACCCGAAC
CAGTCGGCCATCAAGCTGGTGAGCGAGGAGCTCGTGCGCAAGCACGCGGCCCTGCCGCTGTTCAAGCGCGGCGGCCGCCT
CTTCGTGGGCATCTCCGACCCCACCAACAGCCACGCGCTGGACGAGATCAAGTTCCACACCAACCTGGCGGTGGAACCCA
TCCTGGTCGACGACGACAAGATCCGCCGGACGATCGACCACTGGCTGGAGCAGGCCGACGCGCTGGCCGATGCCGTGGCC
GACAGCGAGGGGCTGGACAACCTCGACACCAGCGGTGGCGACGAGGAATTCAACAACGACTCCGGCGTCGACGCGAAGGG
CGACGACACGCCGGTCGTGAAGTTCATCAACAAGGTGCTGGTGGATGCGATCCGCCGCGGCGCCTCGGACATCCACTTCG
AGCCCTACGAAACCGACTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGCAGGTCGCCCGCGTGCCGGTGAAGCTG
CAGGCCCGCATCGCCGCGCGCCTGAAGGTCATGGCGCAGCTGGACATCGCGGAAAAGCGCGTGCCGCAGGACGGCCGCAT
CAAGCTCAACCTGTCGAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCCACGCTGTTCGGCGAAAAGGTCGTGC
TGCGTATCCTGGACGGCGGCGCGGCCAAGCTGGGCATCGACAAGCTCGGTTACGAGGAAGACCAGAAGAAGCTCTTCCTC
GAAGCCGTGCAGAAGCCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGCTCGGGTAAAACCGTGTCGCTGTACACCGC
GCTGAACATCCTCAACGACGACACGCGCAACATCTCCACCGTCGAGGACCCGGTCGAAATCCGCGTCCCGGGCATCAACC
AGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCGGCCCTGCGCAGCTTCCTGCGACAGGACCCGGACGTGATC
ATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGGCATCAAGGCCGCGCAGACCGGCCACATGGTGCTCTCCAC
GCTGCACACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCGTGGCGCCGTACAACATCACCTCCTCGG
TGACCCTCGTCATCGCACAGCGCCTCGCGCGCCGCCTGCACGACTGCAAGCGCGAAGTGCACCTGCCCGACCATGCGCTG
CTCGCCGAAGGGTTCACCTCCGACGAGATCCATGGCGGCATGAAGGTGTACGAGGCGGTCGGTTGTCCCGACTGCACCGA
GGGCTACAAGGGACGCACCGGCATCTACCAGGTGATGCCGATGAGCGAAGAGATCCAGGACATCGTGCTTTCCGGTGGCA
ACGTGATGCAGATCGGCGAGGCCGCGCAACGCTCCGGCGTGCGCGACCTGCGCCAGTCGGCACTGGTCAAGGTCCGGAAT
GGCGTGACAAGCCTCGCGGAAATCAACCGCGTGACGAAAGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.203

98.78

0.545
  pilB Acinetobacter baylyi ADP1

53.887

98.606

0.531
  pilB Legionella pneumophila strain ERS1305867

51.323

98.78

0.507
  pilB Vibrio cholerae strain A1552

52.437

89.373

0.469
  pilF Neisseria gonorrhoeae MS11

50.19

91.638

0.46
  pilB Vibrio parahaemolyticus RIMD 2210633

49.149

92.16

0.453
  pilB Vibrio campbellii strain DS40M4

48.659

90.941

0.443
  pilB/pilB1 Synechocystis sp. PCC 6803

37.568

95.993

0.361